Label-free quantitative proteomic analysis of molting-related proteins of Trichinella spiralis intestinal infective larvae

AbstractMolting is a key step for body-size expansion and environmental adaptation of parasitic nematodes, and it is extremely important forTrichinella spiralis growth and development, but the molting mechanism is not fully understood. In this work, label-free LC –MS/MS was used to determine the proteome differences betweenT. spiralis muscle larvae (ML) at the encapsulated stage and intestinal infective larvae (IIL) at the molting stage. The results showed that a total of 2885T. spiralis proteins were identified, 323 of which were differentially expressed. These proteins were involved in cuticle structural elements, regulation of cuticle synthesis, remodeling and degradation, and hormonal regulation of molting. These differential proteins were also involved in diverse intracellular pathways, such as fatty acid biosynthesis, arachidonic acid metabolism, and mucin typeO-glycan biosynthesis. qPCR results showed that fiveT. spiralis genes (cuticle collagen 14, putative DOMON domain-containing protein, glutamine synthetase, cathepsin F and NADP-dependent isocitrate dehydrogenase) had significantly higher transcriptional levels in 10  h IIL than ML (P 
Source: Veterinary Research - Category: Veterinary Research Source Type: research

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In conclusion, we demonstrate that T. parva infection leads to alterations in the molecular and functional phenotypes of bovine monocytes. Importantly, since these changes primarily occur in lethal infection, they can serve as biomarkers for ECF progression and severity, thereby aiding in the standardization of protection assessment for T. parva candidate vaccines.
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Host Response and Inflammation Source Type: research
Publication date: Available online 10 November 2019Source: Veterinary ParasitologyAuthor(s): Fatemeh Khadir, Tahereh Taheri, Sima Habibzadeh, Farnaz Zahedifard, Elham Gholami, Maryam Heidari-Kharaji, Ahmad Oryan, Sima RafatiAbstractCutaneous leishmaniosis (CL) is a parasitic disease in animals and human with no satisfactory treatments and vaccination. Rapamycin is a potent inhibitor of mammalian target of rapamycin (mTOR) with various applications. Here, the effect of rapamycin alone or in combination with two other drugs, namely amphotericin B (AmB) and glucantime, was investigated against Leishmania tropica infection. In...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research
In this study, an annual follow-up of 85 CaniLeish® vaccinated dogs and 83 non-vaccinated control dogs was performed. Blood samples were taken for all animals at pre-determined time points: before vaccination; immediately before each one of the two following vaccine doses (at 21 days intervals); and then one, four, six, nine and 12 months after finishing the vaccination course. All samples were tested by an in-house ELISA, using a whole promastigote antigen, for the presence of anti-L. infantum antibodies. Humoral response detectable by the used serological diagnostic method was significantly higher in the vaccine grou...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research
Publication date: Available online 30 October 2019Source: Research in Veterinary ScienceAuthor(s): Jia Xu, Ruo Dan Liu, Shao Rong Long, Yan Yan Song, Peng Jiang, Xi Zhang, Jing Cui, Zhong Quan WangAbstractThe aim of this work was to identify the molecular characteristics of a chymotrypsin-like enzyme from Trichinella spiralis (Tschy) and its facilitation of larval penetration into enteral epithelial cells (EECs). The complete Tschy cDNA sequence was cloned and expressed in Escherichia coli BL21. RT-PCR, IIFA and western blotting showed that Tschy was expressed at the T. spiralis muscle larvae (ML), intestinal infective L1 ...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Authors: Nawab A, An L, Wu J, Li G, Liu W, Zhao Y, Wu Q, Xiao M Abstract Infectious diseases are a major challenge for the poultry industry that causes widespread production losses. Thus, management and control of poultry health and diseases are essential for the viability of the industry. Toll-like receptors are best characterized as membrane-bound receptors that perform a central role in immune homeostasis and disease resistance by recognition of pathogen-associated molecular patterns. In response to pathogen recognition, TLRs initiate both innate and adaptive immune responses which may help to develop immunomodu...
Source: International Reviews of Immunology - Category: Allergy & Immunology Tags: Int Rev Immunol Source Type: research
Nanorobots swimming in blood vessels, in silico clinical trials instead of experimenting with drugs on animals and people, remote brain surgeries with the help of 5G networks – the second part of our shortlist on some astonishing ideas and innovations that could give us a glimpse into the future of medicine is ready for you to digest. Here, we’re going beyond the first part with medical tricorders, the CRISPR/Cas-9 gene-editing method, and other futuristic medical technologies to watch for. 11) In silico clinical trials against testing drugs on animals As technologies transform every aspect of healthcare,...
Source: The Medical Futurist - Category: Information Technology Authors: Tags: Artificial Intelligence E-Patients Future of Medicine Future of Pharma Genomics Health Sensors & Trackers 3d printing AI bioprinting blockchain clinical trials CRISPR digital digital health drug development genetics Innovat Source Type: blogs
Abstract Infection with Giardia produces a wide range of clinical outcomes. Acutely infected patients may have no overt symptoms or suffer from severe cramps, diarrhea, nausea and even urticaria. Recently, post-infectious irritable bowel syndrome and chronic fatigue syndrome have been identified as long-term sequelae of giardiasis. Frequently, recurrent and chronic Giardia infection is considered a major contributor to stunting in children from low and middle income countries. Perhaps the most unusual outcome of infection with Giardia is the apparent reduced risk of developing moderate-to-severe diarrhea due to ot...
Source: Advances in Parasitology - Category: Parasitology Authors: Tags: Adv Parasitol Source Type: research
This study aims to examine the immune responses induced in pigs following vaccination (live S48 tachyzoites) and/or challenge with T. gondii oocysts, through the examination of changes in levels of transcription in CD4, CD8α, IFN-γ, IL-12p35, CXCR3, MyD88. The experiment involved four groups of animals; pigs in group 1 (Challenged) (Chal) were challenged orally with (1 × 103 oocysts) on day 28 of the experiment. Pigs in group 2 (Vaccinated /Challenged) (Vac/Chal) were vaccinated (S48 isolate tachyzoites) on day 0, then challenged on day 28. The group 3 (Vaccinated) (Vac) animals were vaccina...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research
Authors: Szuster-Ciesielska A, Wawiórka L, Krokowski D, Grankowski N, Jarosz Ł, Lisiecka U, Tchórzewski M Abstract Malaria remains one the most infectious and destructive protozoan diseases worldwide. Plasmodium falciparum, a protozoan parasite with a complex life cycle and high genetic variability responsible for the difficulties in vaccine development, is implicated in most malaria-related deaths. In the course of study, we prepared a set of antigens based on P-proteins from P. falciparum and determined their immunogenicity in an in vivo assay on a mouse model. The pentameric complex P0-(P1-P2)2 wa...
Source: Journal of Immunology Research - Category: Allergy & Immunology Tags: J Immunol Res Source Type: research
Publication date: December 2019Source: Microbial Pathogenesis, Volume 137Author(s): Amirreza Javadi Mamaghani, Anwar Fathollahi, Adel Spotin, Mohammad mehdi Ranjbar, Meisam Barati, Somayeh Aghamolaie, Maryam Karimi, Niloofar Taghipour, Mohammad Ashrafi, Seyyed Javad Seyyed TabaeiAbstractToxoplasmosis caused by an obligatory intracellular protozoan parasite of Toxoplasma gondii threats a wide spectrum of human and animal hosts. It has been shown that the intensity of the disease in humans depends on the host's immune responses. Immunological investigations on whole protein molecules of T. gondii have shown that these antige...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
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