Isolation of crude fucoidan from Sargassum witghtii using conventional and ultra-sonication assisted methods

Publication date: Available online 18 September 2019Source: Bioactive Carbohydrates and Dietary FibreAuthor(s): Mandakini Devi Hanjabam, Anuj Kumar, C.S. Tejpal, Elavarasan K, Pankaj Kishore, K. Ashok KumarAbstractFucoidan is fucose rich sulphated polysaccharides having diverse biological properties generally found in cell wall matrix of brown seaweeds. Crude fucoidan was extracted from Sargassum wightii, a brown seaweed common along the Indian coastline. A comparison was made between hot water extraction (HWE) and ultra-sonication assisted extraction (UAE) methods. The UAE methods gave higher yield (14.61 g/100 g) than HWE method (10.59 g/100 g). The UAE fucoidan had lighter colour than HWE as indicated by hunter colour values. The water activity values for fucoidans obtained varied from 0.14 to 0.19. The sulphate content was similar in both fucoidans (̴17%). However, HWE fucoidan had higher content of fucose than UAE fucoidan. The FTIR spectra confirmed the presence of sulphate groups in both the fucoidans. The absorption bands at 1245.37 cm−1 and 1294.99 cm−1 in the HWE and UAE fucoidan respectively indicated the S–O stretching due to sulphate groups in the fucose monomer. The thermal transition temperature as obtained by differential scanning calorimetry for HWE (127.3 °C) was lower than UAE fucoidan (131.8 °C). HWE fucoidan had higher DPPH radical scavenging and metal chelating activities than UAE fucoidan. On the other hand, UAE fucoidan had hig...
Source: Bioactive Carbohydrates and Dietary Fibre - Category: Food Science Source Type: research