Modulation of cardiac optogenetics by vitamin A

In this study, we explored whether exogenous vitamin A can be a limiting factor in the light responsiveness of cardiomyocytes‐expressing ChR2. We showed that in cardiomyocytes virally transduced with ChR2 (H134R)‐enhanced yellow fluorescent protein, vitamin A supplements lower than 10 μM significantly increased ChR2 expression. Adding 1 μM vitamin A changed light‐induced transmembrane potential difference significantly, whereas 5 μM dramatically induced membrane depolarization and triggered intracellular calcium elevation. We concluded that vitamin A supp lementation can modulate the efficiency of ChR2 and provide a complementary strategy for improving the performance of optogenetic tools.

Source: BioFactors - September 10, 2019 Category: Biochemistry Authors: Hoda Keshmiri Neghab, Bahram Goliaei, Ali A. Saboury, Gholamreza Esmaeeli Djavid, Majid Pornour, Jun Hong, Michael Grusch Tags: RESEARCH COMMUNICATION Source Type: research