Multiple doses of stem cells maintain urethral function in a model of neuromuscular injury resulting in stress urinary incontinence.

MULTIPLE DOSES OF STEM CELLS MAINTAIN URETHRAL FUNCTION IN A MODEL OF NEUROMUSCULAR INJURY RESULTING IN STRESS URINARY INCONTINENCE. Am J Physiol Renal Physiol. 2019 Aug 14;: Authors: Janssen K, Lin DL, Hanzlicek B, Deng K, Balog BM, van der Vaart CH, Damaser MS Abstract Stress urinary incontinence (SUI) is more prevalent among women who deliver vaginally than women who have had a caesarean section, suggesting that tissue repair after vaginal delivery is insufficient. A single dose of mesenchymal stem cells (MSCs) has been shown to partially restore urethral function in a model of SUI. The aim of this study was to determine if increasing the number of doses of MSCs improves urethral and pudendal nerve function and anatomy. We hypothesized that increasing the number of MSC doses would accelerate recovery from SUI compared to vehicle treatment. Rats underwent pudendal nerve crush and vaginal distension or a sham injury and were treated intravenously with vehicle or one, two or three intravenous doses of 2 million MSCs 1 hour, 7 days and 14 days after injury. Urethral leak point pressure (LPP) testing with simultaneous external urethral sphincter electromyography and pudendal nerve electroneurography were performed 21 days after injury, followed by harvesting the urethrovaginal complex and the pudendal nerve for semiquantitative morphometry of the external urethral sphincter (EUS), urethral elastin, and the pudendal nerve. Two and three...
Source: American Journal of Physiology. Renal Physiology - Category: Physiology Authors: Tags: Am J Physiol Renal Physiol Source Type: research