Role of B1 and B2 Lymphocytes in Placental Ischemia-induced Hypertension.

Role of B1 and B2 Lymphocytes in Placental Ischemia-induced Hypertension. Am J Physiol Heart Circ Physiol. 2019 Aug 09;: Authors: Laule CF, Odean EJ, Wing CR, Root KM, Towner KJ, Hamm CM, Gilbert JS, Fleming SD, Regal JF Abstract Preeclampsia is a prevalent pregnancy complication characterized by new-onset maternal hypertension and inflammation, with placental ischemia as the initiating event. Studies of others have provided evidence for the importance of lymphocytes in placental ischemia-induced hypertension, however contributions of B1 vs. B2 lymphocytes are unknown. We hypothesized that peritoneal B1 lymphocytes are important for placental ischemia-induced hypertension. As an initial test of this hypothesis, the effect of anti-CD20 depletion on both B cell populations was determined in Reduced Utero-placental Perfusion Pressure (RUPP) model of preeclampsia. Anti-murine CD20 monoclonal antibody (5 mg/kg, Clone 5D2) or corresponding mu IgG2a isotype control was administered IP to timed pregnant Sprague Dawley rats on gestation day (GD)10 and 13. RUPP or Sham control surgeries were performed on GD14 and mean arterial pressure (MAP) measured on GD19 from a carotid catheter. As anticipated, RUPP surgery increased MAP and heart rate and decreased mean fetal and placental weight. However, anti-CD20 treatment did not affect these responses. On GD19, B cell populations were enumerated in the blood, peritoneal cavity, spleen and placenta wi...
Source: American Journal of Physiology. Heart and Circulatory Physiology - Category: Physiology Authors: Tags: Am J Physiol Heart Circ Physiol Source Type: research