DiCre-Based Inducible Gene Expression.

DiCre-Based Inducible Gene Expression. Methods Mol Biol. 2019;1971:225-235 Authors: Damasceno JD, Tosi LRO, Santos RERS, Mottram JC Abstract Induction of gene expression is a valuable approach for functional studies since it allows for the assessment of phenotypes without the need for clonal selection. Inducible expression can find a wide range of applications, from the study of essential genes to the characterization of overexpression of genes of interest. Here, we describe a detailed protocol for the use of the DiCre-based inducible gene expression system in Leishmania parasites. This is a tightly regulated induction system that allows for time- and dose-controlled expression of gene products, as rapidly as within 12 h. PMID: 30980306 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research