Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study
Publication date: Available online 1 August 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Lada Živković, Vladan Bajić, Marija Bruić, Sunčica Borozan, Kristina Popić, Dijana Topalović, Juan Santibanez, Biljana Spremo-PotparevićAbstractImmune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 µg/mL, while in posttreatment it was the concentration of 250 µg/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15-60 min), reaching the greatest reduction after 15 and 45 min. Remarkable ·OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell’s repair machinery. Our resu...
Publication date: Available online 3 April 2020Source: The Annals of Thoracic SurgeryAuthor(s): Mehmet Alagoz, Halil Sencer Akdeniz, Ahmet Dolapoglu
Publication date: Available online 3 April 2020Source: The Annals of Thoracic SurgeryAuthor(s): Daniel L. Miller
Abstract Repair of injured tissues requires angiogenesis, the growth of new blood vessels from pre-existing ones. Cutaneous wound healing is a complex and dynamic process by which skin tissue repairs itself after injury; however, how endothelial cells and pericytes form new blood vessels during cutaneous wound angiogenesis remains unclear. We recently developed a fluorescence-based live imaging system to analyze cutaneous wound angiogenesis in adult zebrafish. Employing this system, we found that endothelial cells and pericytes remain in a quiescent state in normal skin tissue, whereas cutaneous injury immediately...
CONCLUSIONS: Despite more comorbidities in the over 65s, this study shows that there is no significant difference in complication rates between laparoscopic and open inguinal hernia repair irrespective of age category. Selection bias for the type of repair and the potential for an alpha error mean larger studies are required to show equivalence. PMID: 32243111 [PubMed - as supplied by publisher]
CONCLUSION: Delayed repair, up to 2 months later, for uncomplicated infant hernia carries a small risk of incarceration but does not increase the rate of strangulation or other complications. PMID: 32243110 [PubMed - as supplied by publisher]
CONCLUSION: Gallbladder perforations are common during laparoscopic cholecystectomy due to traction with forceps or inflammation. Careful inspection for spillage stone should be done. KEY WORDS: Laparoscopic cholecystectomy, Retained Stones, hernia, Trocar Site. PMID: 32242545 [PubMed - in process]
Conclusion. The ankle ligament reconstruction with additional polyester tape augmentation is an effective technique in treating chronic ankle instability with a satisfactory surgical outcome. PMID: 32242523 [PubMed - in process]
Publication date: Available online 3 April 2020Source: Arthroscopy TechniquesAuthor(s): Graeme P. Hopper, Joanne M. Jenkins, Gordon M. Mackay
Publication date: 4–10 April 2020Source: The Lancet, Volume 395, Issue 10230Author(s): The Lancet
Conclusion: Results of this study could be used to facilitate radial force control and longitudinal flexibility enhancement in the design of aortic stent-grafts. PMID: 32233714 [PubMed - as supplied by publisher]