In Vitro Mammalian Cell Mutation Assays Based On Transgenic Reporters: a Report of the International Workshop on Genotoxicity Testing (IWGT)
Publication date: Available online 13 April 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Paul A. White, Mirjam Luijten, Masayuki Mishima, Julie A. Cox, Joleen Hanna, Rebecca Maertens, Edwin P. ZwartAbstractChemical safety evaluations require assessment of genetic toxicity. Transgenic rodent (TGR) assays permit enumeration of mutations in chromosomally-integrated targets contained in shuttle vectors. In order to improve in vitro mutagenicity assessment, and to substantially reduce animal use, in vitro assays using transgenic reporters have been developed. These assays are based on...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - April 14, 2019 Category: Genetics & Stem Cells Source Type: research

Biomarkers of DNA damage in COPD patients undergoing pulmonary rehabilitation: Integrating clinical parameters with genomic profiling
Publication date: Available online 12 April 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Patrizia Russo, Palma Lamonaca, Mirta Milic, Emilio Rojas, Giulia Prinzi, Vittorio Cardaci, LauraVitiello, Stefania Proietti, Alessia Santoro, Massimo Fini, Stefano BonassiAbstractChronic obstructive pulmonary disease (COPD) is a progressive lung disease characterized by severe respiratory symptoms. COPD shows several hallmarks of aging, and an increased oxidative stress, which is responsible for different clinical and molecular COPD features, including an increased frequency of DNA damage. T...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - April 13, 2019 Category: Genetics & Stem Cells Source Type: research

Lymphocytes exposed to vegetables grown in waters contaminated by anticancer drugs: metabolome alterations and genotoxic risks for human health
Publication date: Available online 3 April 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Chiara Russo, Vittoria Graziani, Margherita Lavorgna, Brigida D’Abrosca, Concetta Piscitelli, Antonio Fiorentino, Monica Scognamiglio, Marina IsidoriAbstractWastewater irrigation of crops may be effective to avoid depletion (about 70%) of freshwater resources. However, the use of reclaimed waters containing persistent microcontaminants such as antineoplastic drugs is of high environmental concern. These active compounds may affect human health with potentially severe adverse effects. To ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - April 5, 2019 Category: Genetics & Stem Cells Source Type: research

Isolation of leukocytes from frozen buffy coat for comet assay analysis of DNA damage
Publication date: Available online 31 March 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Siv Kjølsrud Bøhn, Vaineta Vebraite, Sergey Shaposhnikov, Andrew R. CollinsAbstractFrozen buffy coat fractions are often stored in human biomonitoring trials but their use for biomarker purposes has been limited. The purpose of the current study was to study whether frozen buffy coats can be used to monitor DNA damage levels.EDTA blood samples were provided from 9 healthy, non-smoking female volunteers, aged 26-48. Pre-existing DNA damage (strand breaks and oxidised purines) was...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - April 2, 2019 Category: Genetics & Stem Cells Source Type: research

In vitro mammalian cell mutation assays based on the Pig-a gene: A report of the 7th International Workshop on Genotoxicity Testing (IWGT) Workgroup
Publication date: Available online 7 March 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): J.C. Bemis, R.H. HeflichAbstractPig-a gene mutation assays enumerate cells with the glycosylphosphatidylinositol (GPI) anchor-deficient phenotype as a reporter of mutation in the endogenous Pig-a gene. Methods for measuring mutation in this gene are quite well established for in vivo systems. This approach to mutagenicity assessment has now been extended to in vitro mammalian cell-based systems. An expert workgroup from the 7th International Workshop on Genotoxicity Testing tasked with assessi...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - March 31, 2019 Category: Genetics & Stem Cells Source Type: research

Alkaline comet assay results on fresh and one-year frozen whole blood in small volume without cryo-protection in a group of people with different health status
Publication date: Available online 28 March 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Mirta Milić, Ivan Ožvald, Ivana Vinković Vrček, Marijana Vučić Lovrenčić, Višnja Oreščanin, Stefano Bonassi, Emilio Rojas Del CastilloAbstractUsing alkaline comet assay, DNA damage tail length (TL) and tail intensity (TI) parameters were compared between fresh whole blood and 1-year frozen small volume whole blood in EDTA at -80 °C without cryo-preservation. The studied group consisted of 25 volunteers with different health conditions who served as their own ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - March 30, 2019 Category: Genetics & Stem Cells Source Type: research

Role of aneuploidy in the carcinogenic process: Part III of the Report of the 2017 IWGT workgroup on assessing the risk of aneugens for carcinogenesis and hereditary diseases
Publication date: Available online 29 March 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): David Tweats, David Eastmond, Anthony M. Lynch, Azeddine Elhajouji, Roland Froetschl, Micheline Kirsch-Volders, Francesco Marchetti, Kenichi Masumura, Francesca Pacchierotti, Maik SchulerAbstractAneuploidy is regarded as a hallmark of cancer, however, its role is complex with both pro- and anti-carcinogenic effects evident. In this IWGT review, we consider the role of aneuploidy in cancer biology; cancer risk associated with constitutive aneuploidy; rodent carcinogenesis with known chemical a...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - March 30, 2019 Category: Genetics & Stem Cells Source Type: research

The comet assay applied to HepG2 liver spheroids
This study shows that human 3D spheroidal hepatocellular cultures can be successfully applied for genotoxicity testing by the comet assay and represent a promising advanced in vitro model for toxicity testing. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - March 30, 2019 Category: Genetics & Stem Cells Source Type: research

Chemically induced aneuploidy in germ cells. part ii of the report of the 2017 iwgt workgroup on assessing the risk of aneugens for carcinogenesis and hereditary diseases
Publication date: Available online 20 March 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Francesca Pacchierotti, Kenichi Masumura, David Eastmond, Azeddine Elhajouji, Roland Froetschl, Micheline Kirsch-Volders, Anthony Lynch, Maik Schuler, David Tweats, Francesco MarchettiAbstractAs part of the 7th International Workshops on Genotoxicity Testing held in Tokyo, Japan in November 2017, a workgroup of experts reviewed and assessed the risk of aneugens for human health. The present manuscript is one of three manuscripts from the workgroup and reports on the unanimous consensus reache...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - March 21, 2019 Category: Genetics & Stem Cells Source Type: research

The genotoxicity of organic extracts from particulate truck emissions produced at various engine operating modes using diesel or biodiesel (B100) fuel: a pilot study
Publication date: Available online 17 March 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Božena Novotná, Jitka Sikorová, Alena Milcová, Martin Pechout, Luboš Dittrich, Michal Vojtíšek-Lom, Pavel Rossner, Táňa Brzicová, Jan TopinkaAbstractAn analysis of the toxic effects of emissions should reflect real traffic conditions. The exhaust emissions of particulate matter from diesel engines strongly depend on their operating conditions, with low-speed, low-load "urban creep" conditions, common for truck traffic in heav...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - March 18, 2019 Category: Genetics & Stem Cells Source Type: research

In vitro cardiotoxicity evaluation of graphene oxide
In conclusion, the nano-GO caused cardiotoxicity in our in vitro model, with mitochondrial disturbances, generation of reactive species and interactions with DNA, indicating the importance of the further evaluation of the safety of nanomaterials. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - March 15, 2019 Category: Genetics & Stem Cells Source Type: research

Impact of solar UV radiation on amphibians: focus on genotoxic stress
Publication date: Available online 14 March 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): James Eduardo Lago Londero, Maurício Beux dos Santos, André Passaglia SchuchAbstractSolar UV radiation is one of the most important environmental genotoxic factors. Its incidence increased due to stratospheric ozone depletion, climate changes, and deforestation and plays a crucial role in the worldwide decline of the populations of amphibians. Even sublethal effects of UV-induced genotoxicity may cause drastic consequences in the performance and fitness of amphibians. We reviewe...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - March 15, 2019 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: April 2019Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 840Author(s): (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - March 9, 2019 Category: Genetics & Stem Cells Source Type: research

In Vitro Mammalian Cell Mutation Assays Based on the Pig-a Gene: A Report of the International Workshops on Genetic Toxicology (IWGT) Workgroup
Publication date: Available online 7 March 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): J.C. Bemis, R.H. HeflichAbstractPig-a gene mutation assays enumerate cells with the glycosylphosphatidylinositol (GPI) anchor-deficient phenotype as a reporter of mutation in the endogenous Pig-a gene. Methods for measuring mutation in this gene are quite well established for in vivo systems. This approach to mutagenicity assessment has now been extended to in vitro mammalian cell-based systems. An expert workgroup from the International Workshop on Genotoxicity Testing tasked with assessing t...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - March 8, 2019 Category: Genetics & Stem Cells Source Type: research

Assessment of genotoxicity and cholinesterase activity among women workers occupationally exposed to pesticides in tea garden
We examined 143 women subjects including 77 women workers exposed to pesticide in tea garden (exposed) and 66 women subject with no history of occupational exposure to pesticides (non-exposed). The two groups had similar mean ages, BMI and personal habits. The results revealed that exposed group had lower activity of AChE (2.86 ± 0.75 vs. 3.93 ± 0.87 U/ml) BChE (2.02 ± 0.74 vs. 2.60 ± 0.74 U/ml), p 
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - March 6, 2019 Category: Genetics & Stem Cells Source Type: research

Radioprotective properties of food colorant sodium copper chlorophyllin on human peripheral blood cells in vitro
Publication date: Available online 1 March 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Marko Gerić, Goran Gajski, Branka Mihaljević, Saveta Miljanić, Ana-Marija Domijan, Vera Garaj-VrhovacAbstractSodium copper chlorophyllin (CHL) is a food colorant that exhibits many beneficial properties, including potential for use in radiotherapy. Nevertheless, genotoxicity studies investigating radioprotective properties against γ-radiation on human cells are rather scarce. The aim of this study was to assess the cytotoxicity, genotoxicity and induction of malondialdehyde formation ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - March 4, 2019 Category: Genetics & Stem Cells Source Type: research

Targets and mechanisms of chemically induced aneuploidy. part 1 of the report of the 2017 iwgt workgroup on assessing the risk of aneugens for carcinogenesis and hereditary diseases
Publication date: Available online 2 March 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Anthony M. Lynch, David Eastmond, Azeddine Elhajouji, Roland Froetschl, Micheline Kirsch-Volders, Francesco Marchetti, Kenichi Masumura, Francesca Pacchierotti, Maik Schuler, David TweatsAbstractAn aneuploidy workgroup was established as part of the 7th International Workshops on Genotoxicity Testing. The workgroup conducted a review of the scientific literature on the biological mechanisms of aneuploidy in mammalian cells and methods used to detect chemical aneugens. In addition, the current ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - March 4, 2019 Category: Genetics & Stem Cells Source Type: research

Lactobacillus plantarum MON03 counteracts zearalenone génotoxicty in mice: Chromosome aberrations, micronuclei, DNA fragmentation and apoptotique gene expression
This study was conducted to evaluate the ability of lactic acid bacteria, isolated from Tunisia traditional butter, Lactobacillus plantarum MON03 (LP) to protect mice against cytotoxicity and genotoxicity induced by ZEN. Two doses of LP (2 × 109 CFU/L, ∼2 mg/kg and 4 × 109 CFU/L, ∼4 mg/kg) was added alone or in combination with a toxic intragastric ZEN (40 mg/kg representing 8% of LD50) dose daily for 2 wk by oral gavage. The control group received distilled water. The positive control groups received Colchicin (4 mg/kg bw) for the micronucleus ass...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - March 1, 2019 Category: Genetics & Stem Cells Source Type: research

Genotoxic damage in coelomocytes of eisenia andrei exposed to urban soils
Publication date: Available online 23 February 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Flavio Manoel Rodrigues da Silva Júnior, Caroline Lopes Feijo Fernandes, Ronan Adler Tavella, Laiz Coutelle Hoscha, Paulo Roberto Martins BaischAbstractBased on the hypothesis that urban activities can deposit chemical contaminants in soil and consequently have an impact on the vitality of key organisms of the ecosystem, the aim of the present study was to analyse genotoxicity in earthworm’s coelomocytes in urban soil samples in comparison to soil samples from protected areas....
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - February 25, 2019 Category: Genetics & Stem Cells Source Type: research

Horizon scanning for novel and emerging in vitro mammalian cell mutagenicity test systems
Publication date: Available online 21 February 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Stephen J. Evans, Bhaskar Gollapudi, Martha M. Moore, Shareen H. DoakAbstractThe induction of gene mutation within a DNA sequence can result in an adverse impact, altering or preventing gene function. Therefore, in vitro evaluation of mutagenicity is an essential component of the toxicological screening process. A variety of mutagen screening tools are routinely used in genetic toxicology, which are based on selected reporter genes. These assays are however typically labour intensive and i...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - February 22, 2019 Category: Genetics & Stem Cells Source Type: research

High Information Content Assays for Genetic Toxicology Testing: A Report of the International Workshops on Genotoxicity Testing (IWGT)
Publication date: Available online 21 February 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Stephen D. Dertinger, Yukari Totsuka, Jason H. Bielas, Ann T. Doherty, Jos Kleinjans, Masamitsu Honma, Francesco Marchetti, Maik J. Schuler, Veronique Thybaud, Paul White, Carole L. YaukAbstractWe live in an era of ‘big data’, where the volume, velocity, and variety of the data being generated is increasingly influencing the way toxicological sciences are practiced. With this in mind, a workgroup was formed for the 2017 International Workshops on Genotoxicity Testing (IWGT) to ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - February 22, 2019 Category: Genetics & Stem Cells Source Type: research

The comet assay for human biomonitoring: Effect of cryopreservation on DNA damage in different blood cell preparations
This study was designed within the frame of the COST Action hCOMET 15132 (Working Group 6), with the aim of comparing different peripheral blood cell preparations for their feasibility in human biomonitoring studies, using the comet assay for the evaluation of DNA damage. Basal levels of strand breaks/ALS and formamidopyrimidine DNA glycosylase (Fpg) - sites, and H2O2 (500 μM)-induced strand breaks, were measured in whole blood, peripheral blood mononuclear cells - lymphocytes and monocytes - and buffy coat; in fresh and 1, 4 and 12 weeks-frozen samples. The comparison among the fresh preparations showed that the basal ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - February 15, 2019 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: March 2019Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 839Author(s): (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - February 9, 2019 Category: Genetics & Stem Cells Source Type: research

The Response of Escherichia coli to the Alkylating Agents Chloroacetaldehyde and Styrene Oxide
Publication date: Available online 7 February 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Mark M. Muenter, Ariel Aiken, Jadesola O. Akanji, Samir Baig, Sirine Bellou, Alyssa Carlson, Charles Conway, Courtney M. Cowell, Nicholas A. DeLateur, Alexis Hester, Christopher Joshi, Caitlin Kramer, Becky Leifer, Emma Nash, Macee Qi, Meghan Travers, Kelly C. Wong, Man Hu, Na Gou, Roger W. GieseAbstractDNA damage is ubiquitous and can arise from endogenous or exogenous sources. DNA-damaging alkylating agents are present in environmental toxicants as well as in cancer chemotherapy drugs and...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - February 9, 2019 Category: Genetics & Stem Cells Source Type: research

Transcriptional response of murine bone marrow cells to total-body carbon-ion irradiation
Publication date: Available online 26 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Fang Liu, Zhuanzi Wang, Wenjian Li, Yanting WeiAbstractThe need to understand the health effects of heavy ion irradiation is motivated by the use of this modality in radiotherapy and by the potential for exposure during space missions. We have studied the effects of carbon-ion total-body irradiation on the hematopoietic system of the mouse and, in particular, the transcriptional response of bone marrow (BM) cells. Carbon-ion irradiation caused BM cell DNA damage, apoptosis, elevated ROS, an...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 27, 2019 Category: Genetics & Stem Cells Source Type: research

Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to adrenaline in vitro
Publication date: Available online 24 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Djelić Ninoslav, Radaković Milena, Borozan Sunčica, Vesna Dimirijević-Srećković, Pajović Nevena, Vejnović Branislav, Borozan Nevena, Ezgi Eylül Bankoglu, Stopper Helga, Stanimirović ZoranAbstractDiabetes represents one of the major health concerns, especially in developed countries. Some hormones such as the stress hormone adrenaline can induce reactive oxygen species (ROS) and may worsen the diabetes. Therefore, the main aim of the investigation was to find out whether periph...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 25, 2019 Category: Genetics & Stem Cells Source Type: research

Kidney nanotoxicity studied in human renal proximal tubule epithelial cell line TH1
Publication date: Available online 24 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Monika Sramkova, Katarina Kozics, Vlasta Masanova, Iveta Uhnakova, Filip Razga, Veronika Nemethova, Petra Mazancova, Lucyna Kapka-Skrzypczak, Marcin Kruszewski, Marta Novotova, Victor F. Puntes, Alena GabelovaAbstractProgressive expansion of nanomaterials in our everyday life raises concerns about their safety for human health. Although kidneys are the primary organs of xenobiotic elimination, little attention has been paid to the kidneys in terms of nanotoxicological studies up to now. Her...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 25, 2019 Category: Genetics & Stem Cells Source Type: research

Genotoxicity of (+)- and (−)-usnic acid in mice
Publication date: March 2019Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 839Author(s): Ilya Prokopiev, Galina Filippova, Eduard Filippov, Ivan Voronov, Igor Sleptsov, Aliy ZhanataevAbstractUsnic acid, which is the most widespread and well-studied secondary lichen compound, has antibacterial and cytotoxic effects. Usnic acid is present in lichens as the (+)- and (-)-enantiomers, which have different biological activities. We used a DNA-comet assay to determine the genotoxic effect of (+)- and (−)-usnic acid in the liver and kidney cells of mice. The genotoxic effect of usnic acid ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 25, 2019 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: February 2019Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 838Author(s): (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 23, 2019 Category: Genetics & Stem Cells Source Type: research

The application of the Comet assay in fish cell lines
Publication date: Available online 21 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Bojana Žegura, Metka FilipičAbstractThe use of fish models has been proven to be an effective and sensitive tool for the evaluation of genotoxicity of pure compounds and complex mixtures of chemicals in the context of environmental screening of pollutants and hazard assessment in aquatic toxicology. In particular, fish cell lines have been successfully introduced for detection of genotoxic effects and can serve as an alternative to animal testing in preliminary eco-/genotoxicological stud...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 22, 2019 Category: Genetics & Stem Cells Source Type: research

Genotoxicity Of (+)- And (-)-Usnic Acid In Mice
Publication date: Available online 21 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Ilya Prokopiev, Galina Filippova, Eduard Filippov, Ivan Voronov, Igor Sleptsov, Aliy ZhanataevAbstractUsnic acid, which is the most widespread and well-studied secondary lichen compound, has antibacterial and cytotoxic effects. Usnic acid is present in lichens as the (+)- and (-)-enantiomers, which have different biological activities. We used a DNA-comet assay to determine the genotoxic effect of (+)- and (-)-usnic acid in the liver and kidney cells of mice. The genotoxic effect of usnic a...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 22, 2019 Category: Genetics & Stem Cells Source Type: research

Assessing relative sensitivity of marine and freshwater bivalves following exposure to copper: Application of classical and novel genotoxicological biomarkers
Publication date: Available online 18 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Emily L. Vernon, Awadhesh N. JhaAbstractDetermination of relative sensitivity of biota following exposures to contaminants including metals is important for environmental protection. Copper (Cu), although biologically essential can be highly toxic to biota if present at higher concentrations in the natural environment. Given its ubiquitous presence within coastal and inland water bodies, we compared Cu-induced genotoxicity in two ecologically important mussel species, the freshwater Dreisse...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 20, 2019 Category: Genetics & Stem Cells Source Type: research

A comparison of transgenic rodent mutation and in vivo comet assay responses for 91 chemicals
The objective was to compare the sensitivity of the two assays for detecting genotoxicity. Critical aspects of study design and results were tabulated for each dataset. There were fewer datasets from rats than mice, particularly for the TGR assay, and therefore, results from both species were combined for further analysis. TGR and comet responses were compared in liver and bone marrow (the most commonly studied tissues), and in stomach and colon evaluated either separately or in combination with other GI tract segments. Overall positive, negative, or equivocal test results were assessed for each chemical across the tissues...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 20, 2019 Category: Genetics & Stem Cells Source Type: research

Investigation of Spatial and Temporal Variation of Particulate Matter in vitro Genotoxicity and Cytotoxicity in Relation to the Elemental Composition
Publication date: Available online 18 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Gonca Çakmak, Pelin Ertürk Arı, Esra Emerce, Akif Arı, Mustafa Odabası, Roel Schıns, Sema Burgaz, Eftade O. GagaAbstractEven though the outdoor air pollution and its major component Particulate Matter (PM) are recently classified as human carcinogen, attempts to elucidate the underlying mechanisms of PM toxicity are still crucial and continuing with in vitro approaches in various environmental circumstances. Present study investigated the genotoxicity (Comet assay) and the c...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 20, 2019 Category: Genetics & Stem Cells Source Type: research

Periodontitis: Genomic instability implications and associated risk factors
Publication date: Available online 17 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Tatiana T. Borba, Patrícia Molz, Diene S. Schlickmann, Caroline Santos, Caio F. Oliveira, Daniel Prá, Léo Kreather Neto, Silvia I.R. FrankeAbstractPeriodontitis is a bacterial infection characterized by the presence of a dense inflammatory infiltrate, which may result in increased DNA damage and other nuclear/cellular abnormalities. Therefore, it is important to evaluate the periodontal diseases influence on DNA damage and other nuclear/cellular abnomalies formation as ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 18, 2019 Category: Genetics & Stem Cells Source Type: research

Association of BER and NER pathway polymorphism haplotypes and micronucleus frequencies with global DNA methylation in benzene-exposed workers of China: Effects of DNA repair genes polymorphisms on genetic damage
Publication date: Available online 17 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Guang-hui Zhang, Jing-chao Ren, Mengkai Luo, Junpeng Cui, Yanqiu Du, Daokun Yang, Shouming Cui, Xiao Wang, Weidong Wu, Jia Cao, Zhao-lin XiaAbstractObjectiveThe base excision repair (BER) pathway and nucleotide excision repair (NER) pathway play important roles in the repair of benzene-induced genetic damage, and the effects of polymorphisms in these pathways on genetic damage and global DNA methylation are of great interest.MethodsTen single nucleotide polymorphisms (SNPs) in the BER (XRCC...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 17, 2019 Category: Genetics & Stem Cells Source Type: research

Chimeric mice with human hepatocytes: a new system for genotoxicity studies
Publication date: Available online 14 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Chise Tateno, Masahito Fukumuro, Shoji Masumori, Masakazu Kakuni, Yuji Ishida, Takashi Shimada, Makoto HayashiAbstractGenotoxicity assays are characterized by a method, an in vitro or in vivo target, and an endpoint. Many cell types have been used as targets, including bacterial cells, cultured mammalian cells, and rodent cells in vivo. Human cells are the most important target for evaluating the risk to humans associated with exposure to chemicals. Almost exclusively, the human cells used ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 15, 2019 Category: Genetics & Stem Cells Source Type: research

XRCC1 deficiency correlates with increased DNA damage and male infertility
Publication date: Available online 15 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Vertika Singh, Sujit Kumar Mohanty, Priyanka Verma, Arijit Chakraborty, Sameer Trivedi, Singh Rajender, Kiran SinghAbstractHigh fidelity DNA repair is critical to sustain the genomic integrity and quality of developing germ cells. Deficiencies in DNA repair machinery may result in increased DNA damage in germ cell leading to abnormal spermatogenesis and infertility. X-ray repair cross-complementing group 1 (XRCC1) is a testis enriched protein that plays a crucial role in the DNA base excisi...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 15, 2019 Category: Genetics & Stem Cells Source Type: research

Genotoxicity of engineered nanoparticles in higher plants
Publication date: Available online 11 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Manosij Ghosh, Ilika Ghosh, Lode Godderis, Peter Hoet, Anita MukherjeeAbstractNanoparticles (NPs) are an emerging environmental threat. However, studies of NPs in different environmental components are limited. In this review, we discuss studies that have evaluated the genotoxicity of NPs in higher plants. Among the 29 studies reviewed, silver NPs were most studied (n = 7 articles), with fewer studies reporting the genotoxicity of carbon nanotubes (n = 3), titani...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 12, 2019 Category: Genetics & Stem Cells Source Type: research

Cyto/genotoxicological evaluation of hot spots of soil pollution using Allium bioassays in relation to geochemistry
Publication date: Available online 4 January 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Asta Stapulionytė, Violeta Kleizaitė, Raimondas Šiukšta, Donatas Žvingila, Ričardas Taraškevičius, Tatjana ČėsnienėAbstractSoil from industrial and landfill sites affected by anthropogenic activity was screened for implicit negative effects in an Allium test-system in relation to geochemistry. The concentrations of 15 elements were compared to the ecotoxicologically-based soil guideline values. Admitted geoindices were used to classify test-soils according to ris...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - January 5, 2019 Category: Genetics & Stem Cells Source Type: research

18F-FDG PET/CT scanning: Biological effects on patients: Entrance surface dose, DNA damage, and chromosome aberrations in lymphocytes
Publication date: February 2019Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 838Author(s): Akshaya Prasad, Shangamithra Visweswaran, Karthik Kanagaraj, Venkateswarlu Raavi, M. Arunan, E. Venkatachalapathy, S. Paneerselvam, M.T. Jose, Annalakshmi Ozhimuthu, Venkatachalam PerumalAbstractPositron Emission Tomography/Computed Tomography (PET/CT), a combination of PET and CT, is used in tumor staging, therapy planning, and treatment response monitoring. During PET imaging, patients receive low doses of radiation, which can induce an adaptive response and necessitate higher doses for therapeu...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 29, 2018 Category: Genetics & Stem Cells Source Type: research

Editorial Board
Publication date: January 2019Source: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 837Author(s): (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 28, 2018 Category: Genetics & Stem Cells Source Type: research

Scots pine as a promising indicator organism for biomonitoring of the polluted environment: A case study on chronically irradiated populations
Publication date: Available online 26 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Stanislav Geras’kin, Polina Volkova, Denis Vasiliyev, Nina Dikareva, Alla Oudalova, Elizaveta Kazakova, Ekaterina Makarenko, Gustavo Duarte, Alexey KuzmenkovAbstractIn this paper the main results of long-term (2003-2016) observations on Scots pine populations inhabiting sites affected by the Chernobyl accident are presented. Populations growing for many years under chronic radiation exposure are characterized by the enhanced mutation rates, increased genetic diversity, changes in the...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 27, 2018 Category: Genetics & Stem Cells Source Type: research

Evaluation of the DNA damage in lymphocytes, sperm and buccal cells of workers under environmental and occupational boron exposure conditions
This study was conducted in two districts of Balıkesir; Bandırma and Bigadic, which geographically belong to the Marmara Region of Turkey. Bandırma is the production and exportation zone for the produced boric acid and some borates and Bigadic has the largest B deposits in Turkey. 102 male workers who were occupationally exposed to boron from Bandırma and 110 workers who were occupationally and environmentally exposed to boron from Bigadic participated to our study. In this study the DNA damage in the sperm, blood and buccal cells of 212 males was evaluated by comet and micronucleus assays. No significant increase in t...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 24, 2018 Category: Genetics & Stem Cells Source Type: research

Assessment of DNA damage in Polish children environmentally exposed to pesticides
In this study 108 children, living in areas of an intense pesticide use and a control group (n = 92) of children from an agrotouristic area were investigated, whether DNA damage increased due to prolonged pesticide exposure. A presence of DNA breaks and oxidative damage to DNA bases, characterized as Fpg-sensitive sites, were detected by comet assay. Micronuclei (MN) formation was evaluated by cytokinesis-block MN assay. The exposure of children to pesticides resulted in increased number of MN in peripheral blood lymphocytes (P = 0.016), increased DNA strand breaks level (P = 0.002...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 22, 2018 Category: Genetics & Stem Cells Source Type: research

Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro
Publication date: Available online 21 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Dijana Topalović, Dragana Dekanski, Biljana Spremo-Potparević, Andrea Pirković, Sunčica Borozan, Vladan Bajić, Danilo Stojanović, Francesca Giampieri, Massimiliano Gasparrini, Lada ŽivkovićAbstractPhenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and a...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 22, 2018 Category: Genetics & Stem Cells Source Type: research

18F-FDG PET/CT scanning: biological effects on patients: entrance surface dose,: DNA damage, and chromosome aberrations in lymphocytes
Publication date: Available online 19 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Akshaya Prasad, Shangamithra Visweswaran, Karthik Kanagaraj, Venkateswarlu Raavi, M Arunan, E Venkatachalapathy, S Paneerselvam, MT Jose, Annalakshmi Ozhimuthu, Venkatachalam PerumalAbstractPositron Emission Tomography/Computed Tomography (PET/CT), a combination of PET and CT, is used in tumor staging, therapy planning, and treatment response monitoring. During PET imaging, patients receive low doses of radiation, which can induce an adaptive response and necessitate higher doses for thera...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 20, 2018 Category: Genetics & Stem Cells Source Type: research

Manganese oxide nanoparticles induce genotoxicity and DNA hypomethylation in the moss Physcomitrella patens
Publication date: Available online 18 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Ilika Ghosh, Abhishek Sadhu, Yuji Moriyasu, Maumita Bandyopadhyay, Anita MukherjeeGraphical abstract (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 19, 2018 Category: Genetics & Stem Cells Source Type: research

Buccal epithelial cell micronuclei: sensitive, non-invasive biomarkers of occupational exposure to low doses of ionizing radiation
Publication date: Available online 19 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Laura Aguiar Torres, Armindo dos Santos Rodrigues, Diana Linhares, Ricardo Camarinho, Zélia Maria Nunes Páscoa Soares Rego, Patrícia Ventura GarciaAbstractWe have evaluated buccal epithelial cell micronucleus formation as a biomarker of genotoxic damage following occupational exposure to low doses of ionizing radiation. Buccal epithelial cells were collected from 42 occupationally exposed medical personnel and 39 non-exposed individuals (reference group). Each individu...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 19, 2018 Category: Genetics & Stem Cells Source Type: research

Micronuclei frequency in peripheral blood lymphocytes and levels of anti-p53 autoantibodies in serum of residents of Kowary city regions (Poland) with elevated indoor concentrations of radon
ConclusionThe problem of the radon exposure is still unresolved and needs further studies on bigger human cohorts in order to search for more sensitive biomarkers. (Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis)
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 16, 2018 Category: Genetics & Stem Cells Source Type: research