Simplified Purification of AAV and Delivery to the Pancreas by Intraductal Administration.

Simplified Purification of AAV and Delivery to the Pancreas by Intraductal Administration. Methods Mol Biol. 2019;1950:373-387 Authors: Guo P, Wiersch J, Xiao X, Gittes G Abstract Genetic manipulation is a very powerful tool for studying diabetes, pancreatitis, and pancreatic cancer. Here we discuss the use of an adeno-associated virus (AAV) vector to modify gene expression, such as to introduce a green fluorescence protein (GFP) in wild-type mice, cre recombinase in loxP mice, or to inactivate a gene with shRNA. The use of viruses for genetic modification allows for time-specific genetic changes which have advantages over time-consuming and often complex cross-breeding strategies. Here we provide a detailed approach for this process from viral production and purification through pancreatic ductal infusion. Our protocol allows efficient delivery of AAV to mediate GFP or cre expression for cell lineage tracing in the mouse pancreas or for the delivery of transgenes under a specific promoter to these cells. PMID: 30783986 [PubMed - indexed for MEDLINE]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research

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CONCLUSION: This first randomized resistance training trial in pancreatic cancer patients indicated clinically relevant improve- ments in QoL after 3 but not after 6 months. Given the severity of pancreatic cancer, exercise recommendations may already commence at surgery. PMID: 31431236 [PubMed - in process]
Source: Deutsches Arzteblatt International - Category: General Medicine Tags: Dtsch Arztebl Int Source Type: research
In conclusion, the PRPF4 gene plays an important role in the growth of breast cancer cells and is therefore a potential therapeutic target.
Source: Molecular and Cellular Probes - Category: Molecular Biology Source Type: research
Publication date: 15 December 2019Source: Journal of Molecular Structure, Volume 1198Author(s): Lei Wang, Gui-Bin XueAbstractSolvothermal reactions of 1, 3, 5-tris (4-carboxyphenyl)-benzene (H3BTB) with Gd(NO3)·6H2O gave rise to a new highly porous coordination polymer {[Gd(BTB)(H2O)](DMF)(H2O)}n (1) having three dimensional structure. In light of its highly rigid framework as well as the large one-dimensional triangular channel configuration that has abundant open metal sites, we can utilize the resulting activated 1a as effective Lewis catalysts for the acetaldehyde's cyanosilylation at room temperature. The compa...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Publication date: 15 December 2019Source: Journal of Molecular Structure, Volume 1198Author(s): Liji John, Arish Dasan, R. Selwin Joseyphus, I. Hubert JoeAbstractCo(II), Ni(II), Cu(II) and Zn(II) complexes of Schiff base ligand (thio-L-his) derived from thiophene-2-carboxaldehyde (thio) with l-histidine (L-his) were synthesised. The thio-L-his and its metal complexes have been optimized and quantum chemical parameters are calculated using density functional theory. Despite several donor sites in the synthesised thio-L-his, the coordination with metal ion occurred through azomethine nitrogen and carboxylate oxygen as eviden...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Publication date: Available online 20 August 2019Source: Journal of Molecular LiquidsAuthor(s): Sadegh Farhadian, Behzad Shareghi, Farhang Tirgir, Somayeh Reiisi, Neda Gholamian Dehkordi, Lida Momeni, Ehsan HeidariAbstractOver the past decades, anti-cancer drugs consist of cytotoxic drugs have been interested and developed. Despite the great advances in chemotherapy, there is still no certain medical treatment for cancer. During this investigation, a novel 2,5-diketopiperazine derivative 3(R),6(S)-bis (4-hydroxy phenyl)-piperazine-2,5-dione: (BHPPD) was designed, synthesized, and evaluated. The effects on intestinal protea...
Source: Journal of Molecular Liquids - Category: Molecular Biology Source Type: research
In this study, we performed RNA-sequencing analysis of MCF10A cells and identified 1166 genes that were upregulated and 861 genes that were downregulated by TGF-β. Gene set enrichment analysis revealed that focal adhesion and metabolic pathways were the top enriched pathways of the up- and downregulated genes, respectively. Genes in these pathways also possess significant predictive value for renal cancers. Moreover, we confirmed that TGF-β induced expression of MICAL1 and 2, and the histone demethylase, KDM7A, and revealed their regulatory roles on TGF-β-induced cell migration. We also show a critical effec...
Source: Biochimica et Biophysica Acta (BBA) Molecular Basis of Disease - Category: Molecular Biology Source Type: research
Publication date: Available online 22 August 2019Source: Life SciencesAuthor(s): Yang Ge, Bao-lin Liu, Jun-peng Cui, Shu-qiang LiAbstractLivin is an important member of the human inhibitor of apoptosis proteins (IAPs) family. IAPs are proteins with antiapoptotic abilities, and their functions are different from the Bcl-2 (B-cell lymphoma-2) family proteins. However, the precise role of Livin in colon cancer progression remains unclear. The purpose of this study is to assess the effect of overexpression Livin in colon cancer cells and to examine its molecular mechanism. We demonstrated that Livin induced a colon cancer phen...
Source: Life Sciences - Category: Biology Source Type: research
Publication date: Available online 30 July 2019Source: Medical Mycology Case ReportsAuthor(s): Pattriya Chanyachailert, Charussri Leeyaphan, Sumanas Bunyaratavej, Piriyaporn ChongtrakoolAbstractDematiaceous fungi can cause subcutaneous phaeohyphomycosis, an uncommon fungal infection of the dermis and subcutaneous tissues. Medicopsis romeroi is an emerging organism that can infect patients with subcutaneous phaeohyphomycosis, especially immunocompromised patients. The present case involved subcutaneous phaeohyphomycosis caused by Medicopsis romeroi in an 80-year-old Thai male with poorly controlled diabetes, for whom the le...
Source: Medical Mycology Case Reports - Category: Biology Source Type: research
Publication date: Available online 20 August 2019Source: Carbohydrate PolymersAuthor(s): Xin Xie, Lingman Ma, Yiran Zhou, Wen Shen, Duiyue Xu, Jie Dou, Baiyong Shen, Changlin ZhouAbstractA polysaccharide isolated from Strongylocentrotus nudus eggs (SEP) reportedly displays immune activity in vivo. Here, its effect and underlying mechanism in the treatment of pancreatic cancer were investigated. SEP obviously inhibited pancreatic cancer growth by activating NK cells in vitro/vivo via TLR4/MAPKs/NF-κB signaling pathway, The tumor inhibitory rate achieved to 44.5% and 50.8% at a dose of 40 mg/kg in Bxpc-3 and SW1...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
Publication date: 1 December 2019Source: Carbohydrate Polymers, Volume 225Author(s): Ecaterina Stela Dragan, Maria Valentina DinuAbstractMacromolecular drugs, such as proteins and peptides, are lately readily available and used in the treatment of diseases including diabetes and cancer, as well as in therapies such as gene therapy, wound dressing, and tissue engineering. However, the bioavailability, the extent and the rate at which these drugs reach the target tissue are highly dependent on the carrier and on the route of administration. Among the multitude of biocompatible polymers used to design vehicles for macromolecu...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
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