Bioaccumulation of selected macro- and microelements and their impact on antioxidant properties and accumulation of glucosinolates and phenolic acids in in vitro cultures of Nasturtium officinale (watercress) microshoots

Publication date: Available online 16 July 2019Source: Food ChemistryAuthor(s): Marta Klimek-Szczykutowicz, Agnieszka Szopa, Eliza Blicharska, Michał Dziurka, Łukasz Komsta, Halina EkiertAbstractThe study evaluated bioaccumulation capacity of macro- and microelements, their impact on the production of glucosinolates and phenolic acids and antioxidant properties in a microshoot culture model of Nasturtium officinale.Elements: calcium, chromium, copper, iron, lithium, magnesium, selenium and zinc were supplemented in different salt concentrations to culture media. Bioaccumulation of elements [mg/100gDW] varied from 1.24 (Li,1mg/l) to 498.62 (Cr,50mg/l) and was dependent on the type of element and its concentration. The bioconcentration factor (BCF) ranged from 11.37 (Li,25mg/l) to 4467.00 (Ca,1mg/l). The total glucosinolate contents [mg/100gDW] varied from 108.11 (Cr,1mg/l) to 172.90 (Ca,1mg/l). The presence of four phenolic acids was confirmed in the microshoots. Their total contents [mg/100gDW] ranged from 19.35 (Mg,10mg/l) to 139.21 (Fe,50mg/l). The highest antioxidant activity [nM Trolox/mgDW], as evaluated by CUPRAC and QUENCHER-CUPRAC methods, was equal to 55.50 (Cu,1mg/l) and 161.10 (Li,5mg/l), respectively. The results proved good correlations between all studied parameters.

Source: Food Chemistry - July 16, 2019 Category: Food Science Source Type: research