Further insight into the geographic distribution of < i > Leishmania < /i > species in Peru by cytochrome < i > b < /i > and mannose phosphate isomerase gene analyses

by Hirotomo Kato, Abraham G. C áceres, Chisato Seki, Carmen Rosa Silupu García, Carlos Holguín Mauricci, Salvadora Concepción Castro Martínez, Dafne Moreno Paico, Josefa Leila Castro Muniz, Lucinda Doriz Troyes Rivera, Zoila Isabel Villegas Briones, Silvia Guerrero Quincho, Guísela Lucy Sulca Jayo, Edwin Tineo Villafuerte, Carlos Manrique de Lara Estrada, Fernando Rafael Arias, Fredy Santiago Passara, Nancy Ruelas Llerena, Makoto Kubo, Ahmed Tabbabi, Daisuke S. Yamamoto, Yoshihisa Hashiguchi

To obtain further insight into geographic distribution ofLeishmania species in Peru, a countrywide survey, including central to southern rainforest areas where information on causative parasite species is limited, was performed based on cytochromeb (cyt b) and mannose phosphate isomerase (mpi) gene analyses. A total of 262 clinical samples were collected from patients suspected of cutaneous leishmaniasis (CL) in 28 provinces of 13 departments, of which 99 samples were impregnated on FTA (Flinders Technology Associates) cards and 163 samples were Giemsa-stained smears.Leishmania species were successfully identified in 83 (83.8%) of FTA-spotted samples and 59 (36.2%) of Giemsa-stained smear samples. Among the 142 samples identified, the most dominant species wasLeishmania (Viannia) braziliensis (47.2%), followed byL.(V.) peruviana (26.1%), and others wereL.(V.) guyanensis,L.(V.) lainsoni,L.(V.) shawi, a hybrid ofL.(V.) braziliensis andL.(V.) peruviana, andLeishmania (Leishmania) amaz...

Source: PLoS Neglected Tropical Diseases - June 19, 2019 Category: Tropical Medicine Authors: Hirotomo Kato Source Type: research