A new M23-based ELISA assay for anti-aquaporin 4 autoantibodies: diagnostic accuracy and clinical correlation

AbstractPurposeAlthough many assays have been developed to detect anti-aquaporin-4 (AQP4) antibodies, most of these assays require sophisticated techniques and are thus only available at specialized laboratories. The aim of this study was to evaluate the analytical and clinical performance of a new commercial enzyme-linked immunosorbent assay (ELISA RSR, AQP4 Ab Version 2) to detect anti-AQP4 antibodies performed on a fully automated system (SkyLAB 752).MethodsSerum samples from 64 patients with neuromyelitis optica spectrum disorders (NMOSD) (including NMO, longitudinally extensive myelitis-LETM, optical neuritis and myelitis) and 27 controls were tested for anti-AQP4 antibodies. All sera were previously tested using an indirect immunofluorescence (IIF) method on primate tissue, as the reference method. Commercial control sera were used to determine within-run, between-day and within-laboratory precision (CLSI guidelines).ResultsAt a cut-off value of 2.1 U/mL as determined by ROC curves, sensitivity and specificity for NMO were 83.3% and 100%, respectively. The ELISA assay provided 100% concordant results with the reference IIF method. The median concentration of anti-AQP4 antibodies was statistically higher in patients with NMO than in patients with LETM (p  =  0.0006) or with other NMOSD and in controls (p  
Source: Autoimmunity Highlights - Category: Allergy & Immunology Source Type: research

Related Links:

CONCLUSION: In the outpatient clinic specializing in oral anticoagulation, patients add more specific beliefs about the interference of vitamin K-rich diet on coagulation, when compared to those in the general cardiology outpatient clinic. PMID: 31618372 [PubMed - in process]
Source: Revista gaucha de enfermagem EENFUFRGS - Category: Nursing Tags: Rev Gaucha Enferm Source Type: research
CONCLUSION: The Tracer Methodology is used for evaluating and improving the quality of care. PMID: 31618371 [PubMed - in process]
Source: Revista gaucha de enfermagem EENFUFRGS - Category: Nursing Tags: Rev Gaucha Enferm Source Type: research
Publication date: Available online 16 October 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Nicole E. Breuner, Shelby L. Ford, Andrias Hojgaard, Lynn M. Osikowicz, Christina M. Parise, Maria F. Rosales Rizzo, Ying Bai, Michael L. Levin, Rebecca J. Eisen, Lars EisenAbstractThe invasive, human-biting Asian longhorned tick, Haemaphysalis longicornis, was detected in New Jersey in the eastern United States in August of 2017 and by November of 2018 this tick had been recorded from 45 counties across 9 states, primarily along the Eastern Seaboard. The establishment of H. longicornis in the United States has raised the ques...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 16 October 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Jinming Wang, Jifei Yang, Shandian Gao, Aihong Liu, Muhammad Rashid, Youquan Li, Zhijie Liu, Junlong Liu, Guangyuan Liu, Jianxun Luo, Guiquan Guan, Hong YinAbstractBovine theileriosis, caused by protozoan parasites of the genus Theileria, presents with various clinical symptoms. In cattle, clinical presentations and outcomes of bovine theileriosis are closely correlated with the causative Theileria spp. Thus, accurate detection and discrimination of Theileria spp. are essential for epidemiological studies and for provision of ...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 16 October 2019Source: Small Ruminant ResearchAuthor(s): Alison M. Lee, Alan Wolfe, Joseph P. Cassidy, John Moriarty, Ronan O’Neill, Claire Fahy, Emily Connaghan, Chris Cousens, Mark P. Dagleish, Maire C. McElroy, Locksley L. McV. MessamAbstractJaagsiekte sheep retrovirus (JSRV) is the causative agent of ovine pulmonary adenocarcinoma (OPA). JSRV infection is usually detected post-mortem by macroscopic and histological examination of lungs for lesions of OPA. Subsequently, the presence of JSRV may be confirmed using polymerase chain reaction (PCR) on tumour tissue. Our goal was to d...
Source: Small Ruminant Research - Category: Zoology Source Type: research
This study not only represents the most systematic analysis of codon usage patterns in PEDV orf3 genes, but also provides a basic shaping mechanism of the codon usage bias.
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 16 October 2019Source: Research in Veterinary ScienceAuthor(s): Baolin Song, Qianmei Zeng, Yuan Liu, Bangyuan WuAbstractAbundant evidence now supports the idea that methionine deficiency has negative effects on chicken healthy especially in the aspect of cell cycle regulation and apoptosis. But, lacking of knowledge is the evaluation on metabolic organs. To test the effect of methionine deficiency on the kidney, we assessed the apoptosis and the cell cycle of kidney induced by methionine deficiency by the methods of TdT-mediated dUTP Nick-End Labeling (TUNEL), flow cytometry (FCM), quanti...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 16 October 2019Source: Research in Veterinary ScienceAuthor(s): Pascal Rainard
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 16 October 2019Source: Research in Veterinary ScienceAuthor(s): L.C. Rhades, M. Larzábal, A. Bentancor, J. Sabio y García, F.J. Babinec, A. Cataldi, N. Amigo, V.N. Baldone, L. Urquiza, P.J. Delicia, M.C. FortAbstractBovines are the primary reservoir of enterohemorrhagic Escherichia coli (EHEC) O157:H7 and the main source of its transmission to humans. Here, we present a one-year longitudinal study of fecal shedding of E. coli O157. E. coli O157 obtained from recto-anal mucosal samples were characterized by multiplex PCR. The E. coli O157 prevalence ranged from 0.84% in July ...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
PMID: 31617895 [PubMed - in process]
Source: Endocrinology - Category: Endocrinology Authors: Tags: Endocrinology Source Type: research
More News: Allergy & Immunology | Brain | Laboratory Medicine | Neurology | Statistics | Study