Genome and transcriptome analyses of Leishmania spp.: opening Pandora's box.

Genome and transcriptome analyses of Leishmania spp.: opening Pandora's box. Curr Opin Microbiol. 2019 Jun 15;52:64-69 Authors: Cruz AK, Freitas-Castro F Abstract In the last 30 years, significant advances in genetic manipulation tools along with complete genome and transcriptome sequencing have advanced our understanding of the biology of Leishmania parasites and their interplay with the sand fly and mammalian hosts. High-throughput sequencing in association with CRISPR/Cas9 have prepared the ground for significant advances. Given the richness of the progress made over the last decade, in this article, we focused on the most recent contributions of genome-wide and transcriptome analyses of Leishmania spp., which permit the comparison of life cycle stages, the evaluation of different strains and species in their natural niches and in the field and the simultaneously comparison of the gene expression profiles of parasites and hosts. PMID: 31212190 [PubMed - as supplied by publisher]
Source: Current Opinion in Microbiology - Category: Microbiology Authors: Tags: Curr Opin Microbiol Source Type: research

Related Links:

Contributors : Sarah Forrester ; Amy Goundry ; Bruna Torres Dias ; Thyago Leal Calvo ; Milton Os ório Moraes ; Paul M Kaye ; Jeremy C Mottram ; Ana C de Araújo LimaSeries Type : Expression profiling by high throughput sequencingOrganism : Leishmania donovani ; Leishmania infantum ; Mus musculusFIve 6/8 week old female BALB/c mice were infected with the LV9 L. donovani strain,five mice were infected with the L. infantum NCL strain, five were uninfected, and spleen and liver were taken from mice at day 36 post infection. These tissues were then used to prepare for RNAseq
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Leishmania donovani Leishmania infantum Mus musculus Source Type: research
The etiological agent of Chagas disease, Trypanosoma cruzi, is an obligate intracellular parasite that infects an estimated 7 million people in the Americas, with an at-risk population of 70 million. Despite its recognition as the highest impact parasitic infection of the Americas, Chagas disease continues to receive insufficient attention and resources in order to be effectively combatted. Unlike the other parasitic trypanosomatids that infect humans (Trypanosoma brucei and Leishmania spp.), T. cruzi retains an ancestral mode of phagotrophic feeding via an endocytic organelle known as the cytostome-cytopharynx complex (SP...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
B-1 cells are an innate-like population of B lymphocytes that are subdivided into B-1a and B-1b distinguished by the presence or absence of CD5, respectively. B-1 cells can act as regulatory B cells, are able to present antigen and produce IL-10. Leishmaniasis in humans is a complex of diseases caused by parasites of the genus Leishmania. More than 20 species can infect humans, with each species causing the development of different immunological responses in the host. Susceptibility is usually related to the production of anti-inflammatory cytokines while the production of Th1 cytokines is indicative of resistance. However...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
ez-Coloma A Abstract Asteraceae plants from arid lands are a source of biomass, resin and latex rich in terpenoids with diverse biological effects. Thirty-six previously isolated terpenes, comprising sesquiterpenes, diterpenes, triterpenes and quassinoids, isolated from arid-land plants and a series of metabolites from the biotransformation of some lead compounds were evaluated against insect pests (Spodoptera littoralis, Leptinotarsa decemlineata, Myzus persicae and Rhopalosiphum padi), cells (insect, hamster, murine and human tumoral cells) and parasites (Trypanosoma cruzi and Leishmania infantum). Among the ins...
Source: Chemistry and Biodiversity - Category: Biochemistry Authors: Tags: Chem Biodivers Source Type: research
In this study, diverse computational methods were integrated to calculate evolutionary divergence, immunogenicity, IFN-γ production, epitope conservancy, and population coverage of protein fusion models of LeIF-SP15 namely SaLeish. Immunogenicity of LeIF of Leishmania species and SP15 of sandfly saliva has not been investigated in-silico in fusion form. A complete set of 9-mer MHC class I and 15-mer MHC class II peptides were identified with a high affinity for the antigenic epitopes of SaLeish inducing specific responses of CD8+ and CD4+ T cells from BALB/c and human. Our preferred approach was determining truncated...
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research
Nrf2 [nuclear factor erythroid 2-related factor 2 (Nrf2)] regulates the expression of a plethora of genes involved in the response to oxidative stress due to inflammation, aging, and tissue damage, among other pathological conditions. Deregulation of this cytoprotective system may also interfere with innate and adaptive immune responses. Oxidative burst, one of the main microbicidal mechanisms, could be impaired during initial phagocytosis of parasites, which could lead to the successful establishment of infection and promote susceptibility to diseases. A wide diversity of infections, mainly those caused by intracellular p...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
by Vinicius Dantas Martins, Franciele Carolina Silva, Felipe Caixeta, Matheus Batista Carneiro, Graziele Ribeiro Goes, L ícia Torres, Sara Cândida Barbosa, Leonardo Vaz, Nivea Carolina Paiva, Cláudia Martins Carneiro, Leda Quercia Vieira, Ana Maria Caetano Faria, Tatiani Uceli Maioli An association between increased susceptibility to infectious diseases and obesity has been described as a result of impaired immunity in obese individuals. It is not clear whether a similar linkage can be drawn between obesity and parasitic diseases. To evaluate the effect of obesity in the immun e response to cutaneousLe...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Prostaglandins (PG) are lipid mediators derived from arachidonic acid metabolism. They are involved in cellular processes such as inflammation and tissue homeostasis. PG production is not restricted to multice...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
CONCLUSIONS: The venom of Naja naja oxiana revealed remarkable anti-leishmanial effects as a novel anti-parasitic alternative. Thus the bioactive and effective fractions of this venom may be considered as anti-leishmanial candidates in future studies. PMID: 31903885 [PubMed - as supplied by publisher]
Source: Infectious Disorders Drug Targets - Category: Infectious Diseases Authors: Tags: Infect Disord Drug Targets Source Type: research
by Elaheh Davarpanah, Negar Seyed, Fariborz Bahrami, Sima Rafati, Reza Safaralizadeh, Tahereh Taheri Cutaneous leishmaniasisis a vector-borne disease transmitted byLeishmania infected sand flies. PpSP15 is an immunogenic salivary protein from the sand flyPhlebotomus papatasi. Immunization with PpSP15 was shown to protect againstLeishmania major infection.Lactococcus lactis is a safe non-pathogenic delivery system that can be used to express antigensin situ. Here, the codon-optimizedPpsp15-egfp gene was cloned in pNZ8121 vector downstream of the PrtP signal peptide that is responsible for expression and secretion of the pr...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
More News: Biology | Genetics | HIV-Leishmania Co-infection | Microbiology | Parasitic Diseases | Parasitology