Selenoprotein T as a new positive inotrope in the goldfish, Carassius auratus [RESEARCH ARTICLE]

This study aimed to evaluate the cardiac expression of SELENOT, and the effects of exogenous PSELT (a 43–52 SELENOT-derived peptide) on the heart function of C. auratus, a hypoxia tolerance fish model. We found that SELENOT was expressed in cardiac extracts of juvenile and adult fish, located in the sarcoplasmic reticulum (SR) together with calsequestrin-2. Expression increased under acute hypoxia. On ex vivo isolated and perfused goldfish heart preparations, under normoxia, PSELT dose dependently increased stroke volume (VS), cardiac output
and stroke work (SW), involving cAMP, PKA, L-type calcium channels, SERCA2a pumps and pAkt. Under hypoxia, PSELT did not affect myocardial contractility. Only at higher concentrations (10–8 to 10–7 mol l–1) was an increase of VS and
observed. It also reduced the cardiac expression of 3-NT, a tissue marker of nitrosative stress, which increases under low oxygen availability. These data are the first to propose SELENOT 43–52 (PSELT) as a cardiac modulator in fish, with a potential protective role under hypoxia.

Source: Journal of Experimental Biology - June 3, 2019 Category: Biology Authors: Mazza, R., Gattuso, A., Imbrogno, S., Boukhzar, L., Leo, S., Mallouki, B. Y., Filice, M., Rocca, C., Angelone, T., Anouar, Y., Cerra, M. C. Tags: RESEARCH ARTICLE Source Type: research