Rotavirus Gastroenteritis Outbreaks in a Neonate Intermediate Care Unit: Direct Detection of Rotavirus from a Computer Keyboard and Mouse

ConclusionsHand hygiene and an intensive isolation strategy remained the most critical precautions for preventing an outbreak of healthcare-associated viral gastroenteritis in the neonate care unit.
Source: Journal of Microbiology, Immunology and Infection - Category: Microbiology Source Type: research

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Abstract Enteric viruses, generally found in sewage, are recognized as the main cause of waterborne and foodborne public health outbreaks. Among leading enteric viruses, the Rotavirus A (RVA) detection in wastewater appeared to be a novel approach to monitor the emergence of these viruses in some countries where the viral gastroenteritis surveillance is almost absent such as in Tunisia. The RVA detection and quantification in an industrial sewage purification plant of Charguia I (Tunis, Tunisia) were achieved to evaluate the performance of activated sludge procedures coupled to a macrofiltration monolamp ultraviol...
Source: Environmental Science and Pollution Research International - Category: Environmental Health Authors: Tags: Environ Sci Pollut Res Int Source Type: research
In this study, the diagnostic accuracy of InGenius Gastrointestinal Viral (GV) Elite Panel, a newly developed one-step multiplex real-time RT-PCR assay simultaneously detecting rotavirus, adenovirus and astrovirus, was evaluated retrospectively analyzing an archival collection of 128 stool samples of children hospitalized with acute gastroenteritis. The overall sensitivity and specificity for the GV assay was 100% and 96.2% for rotavirus, 96.9% and 100% for astrovirus, 100% and 100% for adenovirus, respectively. The InGenius GV assay showed a high concordance with the reference methods and was able to detect all tested gen...
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research
AbstractA gastroenteritis outbreak occurred in a university in May, 2017, Wuhan, China. The epidemiological survey and pathogen analysis were conducted to identify the pathogen and control this outbreak. Feces or anal swabs from individuals, water, and swabs taken from tap surfaces of the secondary water supply system (SWSS) and foods were collected for the detection of viruses and pathogenic enteric bacteria by real-time RT-PCR and culture, respectively. Nucleotide sequences were determined by RT-PCR and direct sequencing. Genotyping, phylogenetic, and recombination analyses were conducted by a web-based genotyping tool, ...
Source: Food and Environmental Virology - Category: Virology Source Type: research
This study provides the first evidence of distribution of major diarrheal viruses in SW in Dhaka city which is alarming showing grave risk of impending outbreaks through exposure.
Source: Food and Environmental Virology - Category: Virology Source Type: research
Background: Rotavirus is a major causative agent of acute gastroenteritis (AGE) among children worldwide. Outbreaks of rotavirus can occur through transmission from water bodies as a result of contamination with sewage. Studies have shown that environmental surveillance can serve as an early warning system and an effective means of identifying prevalent and emergent rotavirus strains. The aim of this study is to characterize rotavirus strains from sewage effluent in some locations in Nigeria.
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: UMP. 751 Source Type: research
Publication date: Available online 29 June 2018Source: The LancetAuthor(s): Krisztián Bányai, Mary K Estes, Vito Martella, Umesh D ParasharSummaryEnteric viruses, particularly rotaviruses and noroviruses, are a leading cause of gastroenteritis worldwide. Rotaviruses primarily affect young children, accounting for almost 40% of hospital admissions for diarrhoea and 200 000 deaths worldwide, with the majority of deaths occurring in developing countries. Two vaccines against rotavirus were licensed in 2006 and have been implemented in 95 countries as of April, 2018. Data from eight high-income and middle-incom...
Source: The Lancet - Category: General Medicine Source Type: research
Rotavirus was first discovered by Bishop et al in 1973 [1], who described the unique viral particles isolated from the duodenal mucosa of children. Of the seven distinct groups of rotavirus (named A to G), groups A, B, and C have been reported to cause human infections [2]. Rotaviruses, especially those from group A, were considered to be the main cause of viral gastroenteritis (GE) in infants and young children around the world, causing approximately 111 million episodes of GE per year in children under 5 years of age [3].
Source: Journal of Clinical Virology - Category: Virology Authors: Source Type: research
Journal of Medical Virology, EarlyView.
Source: Journal of Medical Virology - Category: Virology Authors: Source Type: research
CONCLUSIONS: This study demonstrates that a biologically active vaccine may produce a low measured VE under a range of epidemiological, vaccine-related and logistical conditions. Low case ascertainment may partly explain the observed heterogeneity in the performance of rotavirus vaccine across different settings, and should be considered in the design and interpretation of future field trials. PMID: 29699788 [PubMed - as supplied by publisher]
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
Abstract From September 2015 to May 2016, epidemic outbreaks of a diarrheal disease caused severe economic losses to the swine industry in northwest China. Typical clinical symptoms of the disease included severe diarrhea, vomiting, dehydration and death. In order to identify the pathogen, 27 intestinal samples were collected from dead piglets in Shaanxi, Gansu and Qinghai provinces and from Ningxia Hui Autonomous Region. All samples were tested using RT-PCR to detect rotavirus (RV), porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV). Twenty-five fecal samples were PEDV positive ...
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research
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