(De)glutamylation and cell death in < i > Leishmania < /i > parasites

by Louise Basmaciyan, Derrick R. Robinson, Nadine Azas, Magali Casanova Trypanosomatids are flagellated protozoan parasites that are very unusual in terms of cytoskeleton organization but also in terms of cell death. Most of the Trypanosomatid cytoskeleton consists of microtubules, forming different substructures including a subpellicular corset. Oddly, the actin netw ork appears structurally and functionally different from other eukaryotic actins. And Trypanosomatids have an apoptotic phenotype under cell death conditions, but the pathways involved are devoid of key mammal proteins such as caspases or death receptors, and the triggers involved in apoptotic induc tion remain unknown. In this article, we have studied the role of the post-translational modifications, deglutamylation and polyglutamylation, inLeishmania. We have shown thatLeishmania apoptosis was linked to polyglutamylation and hypothesized that the cell survival process autophagy was linked to deglutamylation. A balance seems to be established between polyglutamylation and deglutamylation, with imbalance inducing microtubule or other protein modifications characterizing either cell death if polyglutamylation was prioritized, or the cell survival process of autophagy if deglutamylation was prioritized. This emphasizes the role of post-translational modifications in cell biology, inducing cell death or cell survival of infectious agents.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research