135 Defining the T cell landscape and neoantigens via T-cell receptor sequencing and gene expression profiling in cutaneous squamous cell carcinoma

Background: Immune response is key in defense against cutaneous squamous cell carcinoma (cSCC). We aim to better target T cell killing to treat cancer. Methods: CD8+ tumor infiltrating lymphocytes (TILs) obtained from fresh SCC tumor specimens from immunocompetent (n=5) versus organ transplant recipients (OTRs; n=6) were subject to single-cell RNA profiling and T-cell receptor (TCR) sequencing. Data were analyzed using scSeqR. Results: CD8+ TILs were clustered based on gene expression as follows: effector T cells (GZMA, GZMB, IFN- γ); naïve T cells (CCR7, LEF1, TCF7, IL7R); exhausted T cells (BTLA, CTLA4, PDCD1, TIM3, LAG3); and regulatory T cells (FOXP3, STAT3, TNFSRF).

Source: Journal of Investigative Dermatology - April 19, 2019 Category: Dermatology Authors: N.A. Doudican, A. Santana, D. Felsen, A. Khodadadi-Jamayran, A. Tsirigos, J. Carucci Tags: Carcinogenesis and Cancer Genetics Source Type: research