Comparative Genomic Analyses of Municipal Wastewater Salmonella Isolates Detects a Clinically Unreported Salmonellosis Outbreak.

Comparative Genomic Analyses of Municipal Wastewater Salmonella Isolates Detects a Clinically Unreported Salmonellosis Outbreak. Appl Environ Microbiol. 2019 Mar 22;: Authors: Diemert S, Yan T Abstract Municipal wastewater (MW) includes human waste that contains both commensal and pathogenic enteric microorganisms, and this collective community microbiome can be monitored for community diseases. In a previous study, we assessed salmonellosis disease burden using municipal wastewater from Honolulu, Hawaii, which was monitored over a 54-week period. During this time, a Salmonella serovar Paratyphi B var. L(+) tartrate (+) (also known as S. Java) strain was identified: this strain was detected simultaneously to a clinically-reported outbreak, and pulsed field gel electrophoresis (PFGE) patterns were identical between clinical and municipal wastewater isolates. Months after the outbreak subsided, the same pulsotype was detected as the dominant pulsotype in municipal wastewater samples, with no corresponding clinical cases reported. Using genomic characterization - including core single nucleotide polymorphism (SNP) alignment, core genome multi-locus sequence typing (cg-MLST), and screening for virulence and antibiotic resistance genes -all S. Java municipal wastewater isolates were determined to be clonal, indicating a resurgence of the original outbreak strain. This demonstrates the feasibility and utility of municipal wastewater survei...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research