Neutrophil extracellular traps promote peritoneal metastasis of colon cancer cells.

Neutrophil extracellular traps promote peritoneal metastasis of colon cancer cells. Oncotarget. 2019 Feb 08;10(12):1238-1249 Authors: Al-Haidari AA, Algethami N, Lepsenyi M, Rahman M, Syk I, Thorlacius H Abstract Cytoreductive surgery is the only curative option for patients with peritoneal carcinomatosis, however, intraperitoneal recurrence rate is high making new ways to prevent cancer recurrence an urgent need. Recent evidence suggests that neutrophils are involved in cancer progression. The purpose of our study was to examine the role of neutrophils in the spread of colon cancer cells in the peritoneal cavity. The number of metastatic noduli in the peritoneal cavity was quantified in mice injected with murine colon cancer cells (CT-26) intraperitoneally after surgical laparotomy and treated with a neutrophil depleting antibody or DNase I. In addition, peritoneal metastases were harvested from patients with peritoneal carcinomatosis. Scanning and transmission electron microscopy showed extensive neutrophil extracellular trap (NET) formation in peritoneal colon cancer metastases in mice and patients. Neutrophil depletion markedly reduced the number of metastases in laparotomised animals. Administration of DNase I decreased the number of metastatic nodules by 88% in laparotomised animals as well as NET-induced chemokine-dependent colon cancer cell migration and adhesion in vitro. Finally, CT-26 cancer cells were found to express the...
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research