Metformin Treatment Suppresses Melanoma Cell Growth and Motility Through Modulation of microRNA Expression.

Metformin Treatment Suppresses Melanoma Cell Growth and Motility Through Modulation of microRNA Expression.
Cancers (Basel). 2019 Feb 11;11(2):
Authors: Tseng HW, Li SC, Tsai KW
Abstract
Melanoma is a highly aggressive cancer with high mortality in advanced stages.Metformin is an oral biguanide drug used for diabetes and has demonstrated positive effects oncancer prevention and treatment. Herein, we found that metformin significantly suppressedmelanoma cancer cell motility and growth through inducing cell cycle arrest at the G2/M phase andpromoting cell apoptosis. Using the next-generation sequencing approach, we identified threeupregulated microRNAs (miRNA; miR-192-5p, miR-584-3p, and miR-1246) in melanoma cellstreated with metformin. Among these, we examined the roles of miR-192-5p and miR-584-3p anddiscovered that they significantly suppressed melanoma cell motility. Furthermore, they inhibitedmelanoma cell growth through destroying cell cycle progression and inducing cell apoptosis. Usingmicroarray and bioinformatics approaches for identifying putative target genes, Epidermal growthfactor (EGF) containing fibulin-like extracellular matrix protein 1 (EFEMP1) gene for miR-192-5pand an isoform of the secretory carrier membrane proteins (SCAMP3) gene for miR-584-3p could besilenced through targeting their 3'UTR region directly. EFEMP1 and SCAMP3 knockdownsignificantly suppressed melanoma cell growth, but only EFEMP1 knockdown inhibit...

Source: Genomics Proteomics ... - February 11, 2019 Category: Genetics & Stem Cells Authors: Tseng HW, Li SC, Tsai KW Tags: Cancers (Basel) Source Type: research