Trehalose neuroprotective effects on the substantia nigra dopaminergic cells by activating ...

Aims: Trehalose, as a natural disaccharide, is known as an autophagy inducer. The neuroprotective effects of trehalose in the rat model of Parkinson ꞌs disease were the aim of the present study. Main methods: Parkinson's disease model was induced by injecting 6-OHDA in the striatum of male Wistar rats. Apomorphine-induced behavior and substantia nigra neuronal counts were applied to evaluate the neuroprotective effects of trehalose. The autop hagy was studied using the expression of p62 and LC3II/LC3I ratio. In addition, the antioxidant effects of trehalose were assessed by analyzing the levels of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and also glutathione reductase (GR), glutathione peroxidase (GPx) and Catalase (CAT) enzyme s. Moreover, the levels of 3,4-dihydroxyphenylacetic acid (DOPAC) and dopamine (DA) were assessed. Key findings: The behavioral test showed that trehalose in the treatment group reduced the damage to the substantia nigra dopaminergic neurons, which was characterized by improved motor and reduced ro tations in the treatment group as compared with the lesion group. In the histological examinations of the treatment group, trehalose prevented the destruction of dopaminergic neurons. Trehalose treatments increased autophagy (high LC3II/LC3I ratio) and the expression of the p62 protein as well. Thro ugh p62-dependent manner, it led to increased nuclear translocation of Nrf2 transcription factor and elevated expression of downstream antioxidant en...
Source: Iranian Journal of Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research