Gills CYP1A of Oncorhynchus mykiss as a sensitive biomarker of crude oil pollution in freshwater environments

Publication date: Available online 21 January 2019Source: Environmental Toxicology and PharmacologyAuthor(s): Leonardo Ramón Leggieri, Julieta S De Anna, Juan G. Cárcamo, Gerardo A. Cerón, Luis Arias Darraz, Antonella Panebianco, Carlos M. LuquetAbstractThe induction of CYP1 A activity (EROD) and protein expression was compared in liver and gills of rainbow trout from a stream polluted with crude oil, and through laboratory exposures to 1% and 5% of water accommodated fraction of the crude oil (WAF) for 1 and 4 days. Gills EROD increased 1.6-2.7-fold in fish from the polluted stream and during experiments, while liver EROD was induced only by 1% WAF at day 1 (1.5-fold). Contrastingly, crude oil pollution strongly induced both liver and gills CYP1 A protein expression in the field (14-36-fold) and in experiments (4-25-fold). This highlights that crude oil induced CYP1 A activity markedly in gills but only slightly or not at all in the liver, suggesting that differences between organ EROD activities are related to the modulation of CYP1 A enzyme activity but not to the regulation at transcriptional or translational levels.
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research