Phytochemical analysis of Brasolia, Elleanthus, and Sobralia. Three genera of orchids with antibacterial potential against Staphylococcus aureus

Publication date: Available online 17 January 2019Source: Phytochemistry LettersAuthor(s): Max Rykaczewski, Mirosława Krauze-Baranowska, Jerzy Żuchowski, Marta Krychowiak-Maśnicka, Jakub Fikowicz-Krośko, Aleksandra KrólickaAbstractThe purpose of the study was to determine the chemical composition of extracts from three species of orchids: Brasolia cattleya, Elleanthus oliganthus and Sobralia powellii, all belonging to the Sobralieae tribe, evaluate their antistaphylococcal activity and assess possible toxicity towards eukaryotic cells on the model organism Caenorhabditis elegans. The chemical composition of the methanolic extracts from those plants was tested with the use of UHPLC-ESI-MS/MS method. The phytochemical analyses of the extracts revealed presence of three main groups of secondary metabolites: phenolic derivatives, flavonoid glycosides and triterpenoid saponins. In our studies, the presence of C-O-glycosides of methoxylated flavones and ester derivatives of arbutin were found for the first time in the Orchidaceae family. The extracts were investigated for antimicrobial activity against five Staphylococcus aureus strains, including four strains isolated from hospital patients. The antimicrobial activity of the orchid extracts was estimated by the Minimum Inhibitory Concentration (MIC) and the Minimal Bactericidal Concentration (MBC) determined by Broth Microdilutions method. All of the examined extracts showed strong antimicrobial activity against the S. aureus...
Source: Phytochemistry Letters - Category: Chemistry Source Type: research