In vitro Toxicity Assessment of Chitosan Oligosaccharide Coated Iron Oxide Nanoparticles

Publication date: Available online 7 November 2014 Source:Toxicology Reports Author(s): Sudeep Shukla , Alka Jadaun , Vikas Arora , Raj Kumar Sinha , Neha Biyani , V.K. Jain Iron oxide nanoparticles (INPs) have potential biological, biomedical and environmental applications. These applications require surface modification of the iron oxide nanoparticles, which makes it non-toxic, biocompatible, stable and non-agglomerative in natural and biological surroundings. In the present study, iron oxide nanoparticles (INPs) and chitosan oligosaccharide coated iron oxide nanoparticles (CSO-INPs) were synthesized to evaluate the effect of surface coating on the stability and toxicity of nanoparticles. Comparative in vitro cytotoxicity of nanoparticles was evaluated in HeLa, (Human cervix carcinoma), A549 cells (Human lung carcinoma) and Hek 293 (Human embryonic kidney) cells by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay along with flow cytometry study for cell viability, membrane integrity, mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) production. Morphological alteration in nanoparticles treated cells were analyzed by Acridine orange/Ethidium bromide double staining and electron microscopy. Synthesised nanoparticles were found to be spherical in shape, well dispersed and stable at various pH values, making them suitable for biomedical and environmental applications. Present study also indicates that the chitosan o...
Source: Toxicology Reports - Category: Toxicology Source Type: research