Effects of FOSL1 silencing on osteosarcoma cell proliferation, invasion and migration through the ERK/AP ‐1 signaling pathway

Our findings revealed a novel mechanism by which Fos ‐like antigen‐1 (FOS1) depletion played a significantly negative role in the osteosarcoma (OS) progression through the regulation of the extracellular‐signal‐regulated kinase/activator protein‐1 signaling pathway. Functional suppression of FOSL1 might be a future therapeutic strategy regar ding OS. Osteosarcoma (OS), as the most frequent primary malignancy of bone, is characterized by the presence of malignant mesenchymal cells. In the current study, our aim was to explore the possible effects Fos ‐like antigen‐1 (FOSL1) had on the silencing regarding OS cell proliferation, invasion, and migration through the activation of the extracellular‐signal‐regulated kinase (ERK)/activator protein‐1 (AP‐1) signaling pathway. After the collection of OS on top of already having the adjacent n ormal tissue samples, the protein positive expression rate of FOSL1 was then measured by implementing the use of immunohistochemistry and discovered that FOSL1 was robustly expressed in OS. Later, to better grasp the impact FOSL1 projects on OS and its underlying mechanism, we determined the OS rela ted genes as well as the ERK/AP‐1 signaling pathway related genes expression by using a reverse‐transcription quantitative polymerase chain reaction and western blot assay techniques. The results of the aforementioned two experiments revealed that the FOSL1 depletion had downregulated the expres sion of OS related genes by sim...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research