Exposure of pregnant mice to carbon black by intratracheal instillation: Toxicogenomic effects in dams and offspring

Publication date: 14 June 2012 Source:Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 745, Issues 1–2 Author(s): Petra Jackson , Karin S. Hougaard , Ulla Vogel , Dongmei Wu , Lorraine Casavant , Andrew Williams , Mike Wade , Carole L. Yauk , Håkan Wallin , Sabina Halappanavar Exposure to nanomaterials (NM) during sensitive developmental stages may predispose organisms to diseases later in life. However, direct translocation of NM from mother to fetus through the placenta is limited. The present study tests the hypothesis that pulmonary exposure to NM and NM-induced response, such as inflammation during gestation, leads to secondary effects in the fetus. Time-mated C57BL/6BomTac mice were exposed by intratracheal instillation to vehicle (Nanopure water) or one of three concentrations (2.75, 13.5 or 67μg in 40μl Nanopure water) of carbon black Printex 90 (CB) on gestational days 7, 10, 15 and 18, to final cumulative doses of 11, 54 or 268μg/animal. Samples from a subset of male and female newborns were collected on postnatal day 2 (4days after the last maternal exposure) and from dams 26 to 27days post-exposure (post-weaning period). Histopathology, DNA microarrays, pathway-specific RT-PCR arrays, focussed RT-PCR, and tissue protein analysis were employed to characterize pulmonary response in dams exposed to CB during pregnancy. Hepatic gene expression in newborns was interpreted in light of the observed biological responses and ge...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research