Identification and characterization of circular RNAs involved in mechanical force ‐induced periodontal ligament stem cells

Circular RNAs (circRNAs) are a large class of animal RNAs with regulatory potency. Mechanical force (MF) can induce the expression changes of circRNAs in periodontal ligament stem cells (PDLSCs) which may modulate the orthodontic tooth movement process and the alveolar bone remodeling. CircRNAs may serve as miRNAs sponges by regulating one or several miRNA/miRNAs to affect the osteogenic differentiation in MF ‐induced PDLSCs AbstractCircular RNAs (circRNAs) play critical roles in signal transduction during cell proliferation, differentiation, and apoptosis in a posttranscriptional manner. Recently, circRNAs have been proved to be a large class of animal RNAs with regulatory potency. However, whether circRNAs can respond to mechanical force (MF) and impact on human periodontal ligament stem cells (PDLSCs) and the orthodontic tooth movement (OTM) process remain unknown. Here, we investigated the circRNAs expression patterns in PDLSCs induced by MF and found that circRNAs were responsive to the MF in PDLSCs. Through the valid reads ’ distribution analysis, we found that the majority of reads in both the control PDLSCs and the MF‐induced PDLSCs were distributed in exons. Then we analyzed Gene Ontology terms of genes that overlap with or are neighbors of the stress‐responsive circRNAs and found unique enrichment patterns i n biological processes, molecular function, and cellular component of PDLSCs. Next, we predicted the possible functions of circRNAs through circRNAs–m...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research