A systematic review of the use of the alkaline comet assay for genotoxicity studies in human colon-derived cells

Publication date: Available online 30 October 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Ezgi Eyluel Bankoglu, Geema Kodandaraman, Helga StopperAbstractThis review describes the use of the comet assay for assessment of DNA damage in human colon cells. We screened 98 papers, which employed human colon -derived cells to analyse DNA damage induced by different insults with the comet assay. In most cases tumour cell lines were used, and only a few studies were performed with primary colon cells. The comet assay was mostly applied to test chemotherapeutics and natural products. We could not find a clear difference between the susceptibility of cell lines to genotoxic insults and they were all suitable for comet assay experiments. Further comparisons between cell lines, and with primary cells and stem cells would be desirable to understand the relevance of the established cell lines as model for the human target tissue better.
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research