Biogenic Au@ZnO core –shell nanocomposites kill Staphylococcus aureus without provoking nuclear damage and cytotoxicity in mouse fibroblasts cells under hyperglycemic condition with enhanced wound healing proficiency

AbstractThe aim of the present study is focused on the synthesis of Au@ZnO core –shell nanocomposites, where zinc oxide is overlaid on biogenic gold nanoparticles obtained fromHibiscus Sabdariffa plant extract. Optical property of nanocomposites is investigated using UV –visible spectroscopy and crystal structure has been determined using X-ray crystallography (XRD) technique. The presence of functional groups on the surface of Au@ZnO core–shell nanocomposites has been observed by Fourier transforms infrared (FTIR) spectroscopy. Electron microscopy studies reve aled the morphology of the above core–shell nanocomposites. The synthesized nanocomposite material has shown antimicrobial and anti-biofilm activity againstStaphylococcus aureus and Methicillin ResistantStaphylococcus haemolyticus (MRSH). The microbes are notorious cross contaminant and are known to cause infection in open wounds. The possible antimicrobial mechanism of as synthesized nanomaterials has been investigated againstStaphylococcus aureus and obtained data suggests that the antimicrobial activity could be due to release of reactive oxygen species (ROS). Present study has revealed that surface varnishing of biosynthesized gold nanoparticles through zinc oxide has improved its antibacterial proficiency againstStaphylococcus aureus, whereas reducing its toxic effect towards mouse fibroblast cells under normal and hyperglycaemic condition. Further studies have been performed in mice model to understand th...
Source: Medical Microbiology and Immunology - Category: Microbiology Source Type: research