A functional genetic variant in GAS5 lncRNA (rs145204276) modulates p27Kip1 expression and confers risk for gastric cancer.

A functional genetic variant in GAS5 lncRNA (rs145204276) modulates p27Kip1 expression and confers risk for gastric cancer. Br J Biomed Sci. 2018 Oct 01; Authors: Aminian K, Mashayekhi F, Mirzanejad L, Salehi Z Abstract OBJECTIVE: There has been extensive progress in the identification of single nucleotide polymorphisms (SNPs) affecting susceptibility to gastric cancer in recent years. We hypothesised a functional polymorphism (rs145204276) in GAS5 lncRNA (growth arrest specific transcript 5 long noncoding RNA) in gastric cancer that may modulate the p27Kip1 protein level. MATERIAL AND METHODS: We recruited 130 gastric cancer cases and 230 age and sex matched healthy control subjects. Genotyping was performed by tetra-primer amplification refractory mutation system-polymerase chain reaction. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were used to estimate the associations. Expression of p27Kip1 protein was evaluated in gastric cancer tissues by ELISA. RESULTS: The frequencies of the ins/ins, ins/del and del/del genotypes were 67.7%, 27.7% and 4.6% in cases and 54.8%, 36.5% and 8.7% in controls, respectively (p=0.045). The del allele carriers seemed to confer protective effects on gastric cancer risk (OR=0.58; 95%CI= 0.41-0.83; P=0.01). Patients with the del/del genotype showed higher levels of p27Kip1 than patients with the ins/ins genotype (P
Source: British Journal of Biomedical Science - Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

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Publication date: 15 November 2019Source: Life Sciences, Volume 237Author(s): Heresh Moridi, Jamshid Karimi, Heidar Tavilani, Iraj Khodadadi, Amir Nader Emami RazaviAbstractAimsLong non-coding RNAs (LncRNAs) play central roles in the formation and development of gastric cancer (GC). The aim of this study was to evaluate the expression of PURPL and NONHSAT062994 and the relationship between their expressions with clinical characteristics in GC.Main methodsPURPL and NONHSAT062994 LncRNAs and p53 gene expression levels were analyzed both in 50 pairs of cancerous and adjacent noncancerous tissue samples in GC patients using qR...
Source: Life Sciences - Category: Biology Source Type: research
STOMACH cancer outcomes will greatly depend on the how far the cancer the has spread. It is therefore imperative to know the warning signs and get them checked out as soon as possible.
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
ConclusionsAdjuvant therapy is associated with improved OS for patients with gastric GISTs>10cm but provides no benefit in OS for those with GISTs 2-10cm.
Source: The American Journal of Surgery - Category: Surgery Source Type: research
Authors: Guideline Committee of the Korean Gastric Cancer Association (KGCA), Development Working Group &Review Panel Abstract [This corrects the article on p. 1 in vol. 19, PMID: 30944757.]. PMID: 31598379 [PubMed - in process]
Source: Journal of Gastric Cancer - Category: Gastroenterology Tags: J Gastric Cancer Source Type: research
We report a case of a 37-year-old man with a history of recurrent episodes of vomiting and long-standing type 2 diabetes mellitus. Esophagogastroduodenoscopy did not reveal any findings of reflux esophagitis or obstructive lesions. A gastric emptying time scan showed prolonged gastric emptying half-time (344 minutes) indicating delayed gastric emptying. Laboratory tests revealed elevated fasting serum glucose and glycosylated hemoglobin (HbA1c, 12.9%) and normal fasting C-peptide and insulin levels. We performed Roux-en-Y reconstruction after subtotal gastrectomy to treat gastroparesis and improve glycemic control, and the...
Source: Journal of Gastric Cancer - Category: Gastroenterology Tags: J Gastric Cancer Source Type: research
This study evaluated the safety and feasibility of MBBT in patients undergoing TLDG. Thirty-three patients who underwent TLDG with MBBT were retrospectively evaluated. The mean operation time was 277.6±37.1 minutes, including 51.9±15.7 minutes for reconstruction. Two patients had anastomosis-related complications, one patient with stricture after leakage and 1 patient with stenosis. The former patient was treated with endoscopic balloon dilatation, and the latter was managed conservatively; neither required re-operation. MBBT is a safe and feasible technique, with acceptable surgical outcomes. It may be a goo...
Source: Journal of Gastric Cancer - Category: Gastroenterology Tags: J Gastric Cancer Source Type: research
Conclusions: The overlap method was superior to the HDST with respect to anastomotic complications, especially anastomosis stricture. PMID: 31598376 [PubMed]
Source: Journal of Gastric Cancer - Category: Gastroenterology Tags: J Gastric Cancer Source Type: research
Authors: Kim SH, Lee HJ, Park JH, Choi JH, Park SH, Choe HN, Oh SY, Suh YS, Kong SH, Park DJ, Yang HK Abstract Purpose: Gastric cancer with lymph node metastasis (LNM) more than 15 (N3b) was defined as stage IV until the 6th AJCC system. However, it has been reclassified as a localized disease (stage IIb or III) since the 7th system. The aim of this study is to demonstrate that the survival of N3b is comparable to cytology-only positive (CY1-only) stage IV and to propose a new TNM system interpreting N3b as an eligibility criterion for receiving more intensive chemotherapy regimens. Materials and Methods: 1,430...
Source: Journal of Gastric Cancer - Category: Gastroenterology Tags: J Gastric Cancer Source Type: research
Conclusions: The miR-221-3p and miR-122-5p are involved in the development of gastric cancer, and they have important clinical values in gastric cancer diagnosis and prognosis. PMID: 31598374 [PubMed]
Source: Journal of Gastric Cancer - Category: Gastroenterology Tags: J Gastric Cancer Source Type: research
Conclusions: We identified exosomal miRNAs capable of distinguishing between non-malignant and GC-ascites, showing that the combined use of miR-181b-5p and CEA could improve diagnosis. PMID: 31598373 [PubMed]
Source: Journal of Gastric Cancer - Category: Gastroenterology Tags: J Gastric Cancer Source Type: research
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