Elevated serum alkaline phosphatase in epilepsy: Effect of age and treatment.
The objective of the present study was to investigate the effect of antiepileptic drug therapy on the hematobiochemical parameters in patients with epilepsy and to investigate the association of altered parameters with different demographic and clinical variables. Methods: A cross-sectional study involving 567 patients with epilepsy (drug-naive, N=223; phenytoin, N=85; carbamazepine, N=114; valproate, N=100; and multitherapy, N=45) and 37 healthy controls, aged 5-60 years, from North India was conducted. Hematological and biochemical profiling of all the subjects was performed at a single center. Results: Of all the parame...
Source: British Journal of Biomedical Science - September 7, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

GSTT1, Calpain 10 SNP 19 and indices of glycaemia in type 2 Diabetes.
Authors: Osman H, Osman MY, Alsaidy AA PMID: 31460838 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - August 30, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Clinical diagnostic significance of combinations of 14-3-3 η protein, high-mobility group box-1, anti-cyclic citrullinated peptide antibodies, anti-mutated citrullinated vimentin anti-bodies and rheumatoid factor in rheumatoid arthritis.
Conclusions: Despite individual increases in serum 14-3-3η, HMGB1, anti-CCP, anti-MCV and RF, the combination of anti-CCP and anti-MCV might be of great help for diagnostic in RA, and so should be considered as routine tests for this disease. PMID: 31433746 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - August 23, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Rapidly Progressive Case of Type I Madelung Disease with Bilateral Parotid and Minor Salivary Glands Involvement.
We report a case of an obese 56 year old woman who presented with a sudden unexplained progression of swelling in the neck and upper thorax region associated by severe dyspnea. She was admitted with angioedema, and radiology images identified a bilateral adipose degeneration of the parotid and minor salivary glands. Several medical specialists were consulted regarding differential diagnoses such as Cushing's syndrome, but ultimately diagnosed Type 1 Madelung Disease. The patient will be kept under periodic observation and if any deterioration takes place, surgical intervention will be taken under consideration. Despite no ...
Source: British Journal of Biomedical Science - August 21, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Hepatitis C virus core antigen as a possible alternative for evaluation of treatment effectiveness after treatment with direct acting antivirals.
Conclusions: HCVcAg measurement could be an alternative for determining HCV treatment efficacy after chemotherapy and could be an option in the diagnosis of HCV infection. PMID: 31401936 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - August 14, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Role of extracellular matrix remodeling gene SNPs in keratoconus.
Authors: Abdullah OA, El Gazzar WB, Salem TI, Al-Kamil EA Abstract Introduction: Single nucleotide polymorphisms (SNPs) in genes for certain structural components may be implicated in the pathogenesis of keratoconus. We hypothesised links between SNPs in genes coding for collagen, matrix metalloproteinase 9 (MMP9) and tissue inhibitor of matrix metalloproteinase (TIMP) and keratoconus. Furthermore, we hypothesised links between MMP-9 and TIMP-1 SNPs and their tear level in keratoconus patients. Materials and methods: We genotyped 200 keratoconus and 100 control subjects by allele specific PCR, and quantified MMP-9 ...
Source: British Journal of Biomedical Science - August 11, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

IL-6 -572G/C and -174G/C polymorphisms association with hepatitis C virus induced hepatocellular carcinoma.
Authors: Badawy AA, Othman G, Elabbasy LM, Abd Elsalam M, Shrief R, Fahmy EM, Kamel NM, Osman A, Zaki ME PMID: 31314698 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 19, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

miR-15a: A Potential Diagnostic Biomarker and a Candidate for Non-operative Therapeutic Modality for Age-related Cataract.
Authors: Abdullah OA, El Gazzar WB, Salem TI, Elmohamady MN, Nasif SN, Eltaher SM Abstract INTRODUCTION: In order to better understand the role of hsa-miR-15a in the pathogenesis of age-related cataracts, we hypothesised altered expression, and of target anti-apoptotic genes, BCL-2 and MCL-1, in lens epithelial cells amongst age-related cataract patients. MATERIAL AND METHODS: Reverse transcription quantitative polymerase chain reaction (RT-qPCR) quantified the expression of hsa-miR-15a and the target genes BCL-2 and MCL-1 in lens epithelial cells of 120 age-related cataract patients (40 patients with cortical ...
Source: British Journal of Biomedical Science - July 4, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Genotypic analysis of XRCC4 and susceptibility to cervical cancer.
Authors: Gupta MK, Kushwah AS, Singh R, Banerjee M Abstract Background: XRCC4 encodes a DNA repair protein which maintains the genome stability by repairing double-strand breaks by the error-prone method. Defects in the protein-encoding gene leads to impairment of DNA repair process and accumulation of DNA damage, a hallmark of cancer development. We hypothesised that variants in XRCC4 are linked in cervical cancer has been carried out for the prediction of disease susceptibility. Material & Methods: Genotyping of XRCC4 variants viz. intron3 DIP (rs28360071), intron7 DIP (rs28360017), G-1394T(rs6869366) and G-6...
Source: British Journal of Biomedical Science - July 1, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Polymorphisms in GSTM1 and GSTT1 influence the response and treatment outcome in lung cancer patients treated with platinum-based chemotherapy.
CONCLUSION: The present study found no association of GSTT1 and GSTM1 with the overall survival in LC patients, however our data shows that patients carrying the GSTM1 genotype had a good response when treated with platinum-based doublet chemotherapy, therefore suggesting that GSTM1 polymorphism plays an important role in influencing the chemotherapy outcome and response in patients with lung cancer. PMID: 31218944 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 22, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Routine coagulation molecules predict nasopharyngeal carcinoma and associated metastases.
Conclusions: Routine coagulation indices can predict nasopharygeal carcinoma, with the combination of prothrombin time, APTT and PDW being strongest. The combination of APTT, fibrinogen and FDPs provides a useful score to predict metastases. These indices should be considered in the diagnosis and staging of this disease. PMID: 31210089 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 19, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Semi -automated standardisation of melanin bleaching procedures of heavily pigmented melanocytic lesions for immunohistochemical analysis on an automated platform.
Authors: Orchard GE, Gabriel J, Shams M, Fernando P, Satoc J, Nwokie T, Ismail F, d'Amico C Abstract The diagnosis of heavily pigmented melanocytic lesions is problematic. This is often compounded by lack of visibility of nuclear detail of tumour cells due to physical masking by melanin pigment. Similarly there can be colour merging of chromogenic final reaction products with melanin, making evidence of antigenic localisation problematic. There are a number of melanin bleaching techniques available, the most widely employed method for immunohistochemical assessments is the use of dilute hydrogen peroxidase. All met...
Source: British Journal of Biomedical Science - June 1, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of 32-bp deletion polymorphism and promoter methylation of PTEN and hepatitis C virus induced hepatocellular carcinoma.
Authors: Badawy AA, El-Rabat A, Elshazly TA, Ali SA, Alruwaili AM, Gad DF, Dawoud MG, Elmasry EE, Zaki ME PMID: 31109254 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 23, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Evaluation and comparison of the diagnostic performance of routine blood tests in predicting liver fibrosis in chronic hepatitis B infection.
Authors: Lu W, Zhang YP, Zhu HG, Zhang T, Zhang L, Gao N, Chang DY, Yin J, Zhou XY, Li MY, Li YT, Li ZZ, He Q, Geng Y Abstract BACKGROUND & AIMS: Biopsy is the gold standard for staging liver fibrosis, but it may be accompanied by complications. As an alternative, non-invasive markers such as transient elastography (for liver fibrosis) and certain combinations of routine blood markers (liver function tests, full blood count) have been developed although their clinical significance remains controversial. Here, we compare the diagnostic values of non-invasive markers in patients with chronic hepatitis B infection...
Source: British Journal of Biomedical Science - May 9, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Genetic variants of XRCC1 and risk of hepatocellular carcinoma in chronic hepatitis C patients.
CONCLUSION: We conclude that the T-allele of c.1254C>T (rs2293035) and the C allele of c.1517G>C (rs139599857) genetic variants may be associated with increased HCC risk among chronic hepatitis C patients. PMID: 31025604 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - April 28, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Identification and characterisation of anti - Pseudomonas aeruginosa proteins in mucus of the brown garden snail, Cornu aspersum.
CONCLUSIONS: The study has identified novel proteins with antimicrobial properties which could be used to develop treatments for use in human medicine. PMID: 30966874 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - April 12, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The rivaroxaban-adjusted normalized ratio: Use of the prothrombin time to monitor the therapeutic effect of rivaroxaban.
CONCLUSION: Using a rivaroxaban-specific NR effectively minimises inter-thromboplastin variability. By utilizing NR-rivaroxaban, the standardized prothrombin time results could be rapidly obtained, especially useful in standardising the therapeutic effect of rivaroxaban. PMID: 30967043 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - April 12, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Clinical significance of serum miR-25 in non-small-cell lung cancer.
Authors: Li J, Yu M, Liu Z, Liu B PMID: 30919763 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - March 30, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A panel of a mitogenic (PDGF), biochemical (albumin) and demographic (age) parameters for the noninvasive assessment of hepatic fibrosis.
Authors: Attallah AM, Albannan MS, Omran MM, Zayed R, Saif S, Farid A, Hassany M, Yosry A, Omran D Abstract BACKGROUND: Several studies have investigated certain fibrosis markers that incorporate liver function tests, fragments of liver-matrix components, and/or degraded products generated by hepatic stellate cells for determining the degree of hepatic fibrosis. However, the role of these molecules in the development of hepatic fibrosis is unclear. This work aimed (a) to determine whether levels of platelet-derived growth factor (PDGF) are linked to different stages of hepatic fibrosis, and (b) investigate its diag...
Source: British Journal of Biomedical Science - March 30, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

ABCB1 2677G > T single nucleotide polymorphism influences warfarin dose requirement for warfarin maintenance therapy.
ABCB1 2677G>T single nucleotide polymorphism influences warfarin dose requirement for warfarin maintenance therapy. Br J Biomed Sci. 2019 Mar 25; Authors: Gopisankar MG, Hemachandren M, Surendiran A Abstract This work represents an advance in biomedical science because it shows, in a singlecentre study of 210 patients, an association between ABCB1 2677 G>T genetic polymorphism and the maintenance dose requirement of warfarin for patients who have achieved a stable INR. Hence incorporation of this polymorphism to algorithms for dose prediction of warfarin may improve the accuracy of dose predictio...
Source: British Journal of Biomedical Science - March 27, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Interaction of antioxidant gene variants and susceptibility to Type 2 Diabetes Mellitus.
CONCLUSION: As the number of gene combinations increase, there is a rise in odds ratio showing disease risk, suggesting that gene-gene interaction plays an important role in T2DM susceptibility. Individuals who possess the GSTM1del, GSTT1del, GSTP1 105I/V(+313A/G), CAT-21A/T, SOD2+47C/T and GPx1+599C/T are at very high risk of developing T2DM. PMID: 30900957 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - March 23, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Pushing the boundaries of in situ hybridisation for mRNA demonstration: Demonstration of kappa and lambda light chain restriction in follicular lymphoma.
Authors: Warford A, Rahman M, Hughes JA, Gerrard G, Ribeiro DA PMID: 30892145 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - March 22, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Long noncoding RNA Xist predicts the presence of lymph node metastases in human esophageal squamous cell carcinoma.
Authors: Li J, He D PMID: 30883269 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - March 20, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of GSTP1 gene polymorphism with diabetic nephropathy and co-morbidities (hypertension and dyslipidemia) in Type 2 diabetes mellitus.
Authors: Abbas S, Raza ST, Mir SS, Siddiqi Z, Mahdi F PMID: 30883298 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - March 20, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Genetic polymorphisms in DNA repair genes and their association with cervical cancer.
CONCLUSION: The XRCC1+399A/G SNP is linked with cervical cancer. We suggest that this variant can be utilized as a prognostic marker for determination of cervical cancer susceptibility. PMID: 30870085 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - March 16, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Immunohistochemical Detection of V600E BRAF Mutation is a Useful Primary Screening Tool for Malignant Melanoma.
CONCLUSION: The data revealed a close link between the two methods, supporting the use of the V600E as a primary screen for BRAF mutations in malignant melanoma. Negative results would be recommended for DNA probe testing. V600E antibody is considerably cheaper than the DNA probe assay. The BRAF IHC test can be performed within 24-48 hours, faster than the DNA probe assay enabling patients to receive faster treatment with improved turnaround times. Finally IHC detection is conserving of patient DNA from tumour blocks as only one section is required to perform the assay. PMID: 30870099 [PubMed - as supplied by publisher...
Source: British Journal of Biomedical Science - March 16, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Clinicopathological impact of Anti Smooth Muscle Antibodies in patients with Nonalcoholic Fatty Liver Disease.
Authors: Elalfy H, El-Maksoud MA, Abed S, El Aziz MA, Elsamanoudy AZ, Abo-Khair S, Mohamed MA, Elkashef W, Zalata K, Farag R, Arafa M Abstract INTRODUCTION: Previous studies examining the prevalence of serum autoantibodies in patients with NAFLD reported a wide range of prevalence rates depending on the threshold values used for an abnormal titer. We aimed to evaluate the association of anti-smooth muscle antibodies (ASMAbs) with pathological changes including liver fibrosis in NAFLD patients. PATIENTS AND METHODS: 124 patients were enrolled and classifed on ASMAbs positive (>1/40) or negative (= or
Source: British Journal of Biomedical Science - February 1, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The Association of Hepatic Fat Percentage With Selected Anthropometric And Biochemical Parameters At 3 Tesla Magnetic Resonance Imaging.
CONCLUSIONS: The multi-parametric approach of MRI, biochemical and anthropometric measurements could be adopted to identify subjects at risk of developing non-alcoholic fatty liver disease at early stage. PMID: 30691359 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 31, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Lactobacillus casei suppresses hfq gene expression in Escherichia coli O157:H7.
Conclusion This result showed that probiotic bacteria not only could effect on pathogens bacteria by bacteriocins it could down-regulate the toxin and virulence genes as well. PMID: 30633636 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 12, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

BMP4 circulating levels and promoter (rs17563) polymorphism in risk prediction of idiopathic male infertility.
CONCLUSION: BMP4 gene polymorphism and its circulating levels is related to idiopathic male infertility. PMID: 30628543 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 11, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Inverse Relationship Between Helicobacter pylori cagG Pathogenicity Island and Risk of Gastric Ulcer.
Authors: Bakhti SZ, Raei N, Latifi-Navid S, Zahri S, Yazdanbod A PMID: 30616430 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 10, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A single nucleotide polymorphism in TLR4 is linked with the risk of HIV-1 infection.
CONCLUSION: TLR4 (Asp299Gly) gene polymorphism is a risk factor in HIV-1 disease. PMID: 30569830 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 22, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The severity of NAFLD is associated with the risk of urolithiasis.
CONCLUSIONS: The severity of NAFLD is associated with the risk of urolithiasis among NAFLD patients. Moreover, a three-variable model (obesity, APRI score, serum uric acid) could serve as a useful tool for identifying individuals at high risk for urolithiasis in patients with NAFLD. PMID: 30430908 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - November 16, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

TLR3 and TLR4 SNP variants in the liver disease resulting from hepatitis B virus and hepatitis C virus infection.
Authors: Sghaier I, Zidi S, Mouelhi L, Ghazoueni E, Brochot E, Almawi WY, Loueslati BY Abstract BACKGROUND: Chronic infection with hepatitis B (HBV) and C virus (HCV) is linked with a pro-inflammatory state, predisposing to cirrhosis and liver cancer, particularly hepatocellular carcinoma (HCC). A role for toll-like receptor (TLR) signalling in hepatocarcinogenesis was recently documented. We hypothesised a link TLR3 and TLR4 polymorphisms and HCC, as surrogates for the significance of TLR signalling in the promotion and initiation of HCC. MATERIALS & METHODS: We recruited 174 HCV-infected patients, 100 HBV...
Source: British Journal of Biomedical Science - November 15, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Genetic polymorphism of glial cell derived neurotrophic factor (GDNF) in male infertility.
Authors: Shabani S, Mashayekhi F, Shahangian SS, Salehi Z PMID: 30406716 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - November 9, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Influence of single nucleotide polymorphism in IL-27 and IL-33 genes on breast cancer.
Authors: Fathi Maroufi N, Matin M, Ghanbari N, Khorrami A, Amini Z, Haj Azimian S, Isazadeh A, Taefehshokr S, Taefehshokr N, Baradaran B PMID: 30406733 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - November 9, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Clinical diagnostic significance of prealbumin, cholinesterase and retinol binding protein in liver cirrhosis combined with encephalopathy.
Authors: Tan L, Meng Y, Zeng T, Wang Q, Long T, Wu S, Guan X, Fu H, Zheng W, Tian Y, Chen J, Yu J, Wu Y, Li H, Cao L Abstract OBJECTIVE: Hepatic encephalopathy is a common consequence of liver cirrhosis, but diagnosis can be difficult as it is based on clinical criteria alone. We hypothesised that serum prealbumin, cholinesterase and retinol binding protein (RBP) can help support the diagnosis of hepatic encephalopathy. METHODS: We enrolled 306 cirrhotic patients (110 with encephalopathy), 100 chronic hepatitis B patients and 50 healthy controls, measuring routine liver function tests (ALT, AST, GGT, ALP, and b...
Source: British Journal of Biomedical Science - November 6, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Nitrosative Stress and cytokines are linked with the severity of sepsis and organ dysfunction.
Authors: Kumar S, Gupta E, Srivastava VK, Kaushik S, Saxena J, Goyal LK, Mehta S, Jyoti A Abstract OBJECTIVE: An imbalance in oxidant-antioxidant status may impact into the severity of sepsis. We hypothesised links between nitrosative stress and pro-inflammatory cytokines and their correlation with the severity of sepsis and associated organ dysfunction. METHODS: The hypothesis was tested in 110 patients with sepsis (in whom a disease severity score (APACHE II), and assessment of organ failure score (SOFA) were determined) and 55 healthy volunteers. Neutrophil inducible nitric oxide synthase (iNOS) expression a...
Source: British Journal of Biomedical Science - November 2, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

British Journal of Biomedical Science in 2018 What have we learned?
Authors: Blann A Abstract In 2018 the British Journal of Biomedical Science published one guideline (in reproductive science) and 40 research articles in the various disciplines the comprise biomedical science. The latter were 24 original articles and 16 'In Brief' short reports. Of these, 23 are of note to only one of the sub-disciplines (seven each to biochemists and microbiologists, six to cell pathologists, and one each to cytologists, immunologists and reproductive scientists). Reflecting the increasing complexity of laboratory science, thirteen papers crossed one boundary (three papers each relevant to bioche...
Source: British Journal of Biomedical Science - October 10, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A functional genetic variant in GAS5 lncRNA (rs145204276) modulates p27Kip1 expression and confers risk for gastric cancer.
Authors: Aminian K, Mashayekhi F, Mirzanejad L, Salehi Z Abstract OBJECTIVE: There has been extensive progress in the identification of single nucleotide polymorphisms (SNPs) affecting susceptibility to gastric cancer in recent years. We hypothesised a functional polymorphism (rs145204276) in GAS5 lncRNA (growth arrest specific transcript 5 long noncoding RNA) in gastric cancer that may modulate the p27Kip1 protein level. MATERIAL AND METHODS: We recruited 130 gastric cancer cases and 230 age and sex matched healthy control subjects. Genotyping was performed by tetra-primer amplification refractory mutation sys...
Source: British Journal of Biomedical Science - October 3, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of SDF1 β (G801A) and GNB3 (C825T) polymorphisms with the incidence and severity of coronary artery disease.
Association of SDF1β (G801A) and GNB3 (C825T) polymorphisms with the incidence and severity of coronary artery disease. Br J Biomed Sci. 2018 Sep 26; Authors: Eba A, Raza ST, Abbas M, Rizvi S, Rajput M, Mahdi F Abstract Background Coronary artery disease (CAD) is one of the most common heart diseases worldwide, with elevated mortality. CAD is mainly caused by underlying disorder of atherosclerosis, which may be genetically determined. We hypothesised an association of SDF-1β G801A (rs1801157) and GNB3 C825T (rs5443) single nucleotide polymorphisms (SNPs) with the presence and severity of CAD....
Source: British Journal of Biomedical Science - September 28, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Role of variants rs5030717 and rs5030718 of TLR4 in the risk prediction of nephropathy, hypertension and dyslipidaemia in type 2 diabetes mellitus.
CONCLUSION: TLR4 polymorphisms rs5030717 and rs5030718 may be useful in predicting those type 2 diabetics who are at risk of hypertension, nephropathy and/or dyslipidaemia. PMID: 30211669 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - September 15, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

microRNA-935 is reduced in non-small-cell lung cancer tissue, is linked poor outcome, and acts on signal transduction mediator E2F7 and the AKT pathway.
Authors: Wang C, Li S, Xu J, Niu W, Li S Abstract BACKGROUND: A potential role for microRNA-935 (miR-935) has been identified in several cancers but not in non-small cell lung cancer (NSCLC). We hypothesised changes in miR-935 in NSCLC, and proposed mechanisms that may further explain its role in carcinogenesis. METHODS: NCSLC tissue and nearby normal tissue was obtained from 101 patients and was probed by qRT-PCR for miR-935 expression. The role of miR-935 and a potential target (signal transduction factor E2F7) was determined in cell lines by a dual luciferase assay. The function of miR-935 was investigated t...
Source: British Journal of Biomedical Science - September 13, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of Genetic Polymorphisms of Chemokines and their Receptors with clearance or persistence of hepatitis C virus infection.
Authors: El-Bendary M, Neamatallah M, Elalfy H, Besheer T, El-Setouhy M, Mahfouz M, Zein M, Elhammady D, Hegazy A, Esmat G Abstract BACKGROUND: Polymorphisms of certain genes may have an effect on either persistence of infection or spontaneous clearance of hepatitis C virus (HCV). We hypothesized that one or more variants of chemokines (CCL2 and CCL5) and chemokine receptors (CCR2) genes are associated with the susceptibility to HCV infection. METHODS: We recruited 1460 patients with chronic HCV (CHC), 108 subjects with spontaneous virus clearance (SVC), and 1446 individuals as a healthy control group. All were...
Source: British Journal of Biomedical Science - September 5, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

BPI-ANCA in chronic obstructive pulmonary disease with pulmonary Pseudomonas aeruginosa colonization: a potentially novel indicator for poor prognosis.
Authors: Tian Y, Zeng T, Tan L, Wu Y, Yu J, Huang J, Pei Z PMID: 30105943 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - August 16, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

SNP rs10800708 within the KIF14 miRNA binding site is linked with Breast Cancer.
Authors: Shasttiri A, Rostamian Delavar M, Baghi M, Dehghani Ashkezari M, Ghaedi K PMID: 30092718 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - August 12, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Serum leptin and Homeostasis Model Assessment-IR as novel predictors of early liver fibrosis in chronic hepatitis B virus infection.
Authors: Mousa N, Abdel-Razik A, Sheta T, Shabana W, Zakaria S, Awad M, Abd Elsalam M, El-Wakeel N, Elkashef W, Effat N, Elgamal A, G Deiab A, Eldars W Abstract BACKGROUND: The relationship between hepatitis B virus (HBV) infection, leptin and insulin resistance remains unclear. We hypothesised links between serum leptin and insulin resistance in non-diabetic patients with chronic viral hepatitis B infection and their relation to liver fibrosis. METHODS: We recruited 190 untreated patients with chronic HBV infection and 72 healthy controls. Serum leptin, fasting glucose, insulin, liver function tests (LFTs), C-...
Source: British Journal of Biomedical Science - August 7, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Prognostic significance of soluble major histocompatibility complex class I-related chain A (sMICA) in gastric cancer.
Authors: Xu Y, Chen D, Cheng H Abstract OBJECTIVE: Gastric cancer is a common malignancy, but there are no reliable blood markers for this disease. We tested the hypothesis of a link between serum soluble major histocompatibility complex class I-related chain A (sMICA) and clinicopathological features in gastric cancer. METHODS: A total of 196 patients who underwent gastrectomy and 120 controls (46 healthy individuals, 41 minimal gastritis, 7 gastric ulcers and 26 normal appearance of the gastric mucosa on gastroscopic examination) were enrolled. Serum levels of sMICA were assessed by ELISA. The link between sM...
Source: British Journal of Biomedical Science - July 31, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Monocyte/Granulocyte to Lymphocyte ratio and the MELD score as Prognostic Predictors for Early Recurrence of Hepatocellular Carcinoma after Trans-Arterial Chemoembolization.
Authors: Elalfy H, Besheer T, El-Maksoud MA, Farid K, Elegezy M, El Nakib AM, El Aziz MA, El-Khalek AA, El-Morsy A, Elmokadem A, Elsamanoudy AZ, El-Bendary MA Abstract BACKGROUND: The first-line treatment option for intermediate-stage hepatocellular carcinoma is transarterial chemoembolization. Certain blood indices, such as lymphocyte/monocyte ratio (LMR), lymphocyte count, neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR), monocyte-granulocyte/lymphocyte ratio (MGLR) and red blood cell distribution width (RDW) are prognostic biomarkers in certain diseases. The model for end-stage liver disease (M...
Source: British Journal of Biomedical Science - July 13, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Development of a multiplex two-gene real-time PCR assay for accurate detection of Klebsiella pneumoniae.
CONCLUSION: We report a high sensitive and specific multiplex PCR assay for rapid and accurate diagnosis of K. pneumoniae. PMID: 29991336 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 13, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research