British Journal of Biomedical Science in 2018 What have we learned?
Authors: Blann A Abstract In 2018 the British Journal of Biomedical Science published one guideline (in reproductive science) and 40 research articles in the various disciplines the comprise biomedical science. The latter were 24 original articles and 16 'In Brief' short reports. Of these, 23 are of note to only one of the sub-disciplines (seven each to biochemists and microbiologists, six to cell pathologists, and one each to cytologists, immunologists and reproductive scientists). Reflecting the increasing complexity of laboratory science, thirteen papers crossed one boundary (three papers each relevant to bioche...
Source: British Journal of Biomedical Science - October 10, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A functional genetic variant in GAS5 lncRNA (rs145204276) modulates p27Kip1 expression and confers risk for gastric cancer.
Authors: Aminian K, Mashayekhi F, Mirzanejad L, Salehi Z Abstract OBJECTIVE: There has been extensive progress in the identification of single nucleotide polymorphisms (SNPs) affecting susceptibility to gastric cancer in recent years. We hypothesised a functional polymorphism (rs145204276) in GAS5 lncRNA (growth arrest specific transcript 5 long noncoding RNA) in gastric cancer that may modulate the p27Kip1 protein level. MATERIAL AND METHODS: We recruited 130 gastric cancer cases and 230 age and sex matched healthy control subjects. Genotyping was performed by tetra-primer amplification refractory mutation sys...
Source: British Journal of Biomedical Science - October 3, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of SDF1 β (G801A) and GNB3 (C825T) polymorphisms with the incidence and severity of coronary artery disease.
Association of SDF1β (G801A) and GNB3 (C825T) polymorphisms with the incidence and severity of coronary artery disease. Br J Biomed Sci. 2018 Sep 26; Authors: Eba A, Raza ST, Abbas M, Rizvi S, Rajput M, Mahdi F Abstract Background Coronary artery disease (CAD) is one of the most common heart diseases worldwide, with elevated mortality. CAD is mainly caused by underlying disorder of atherosclerosis, which may be genetically determined. We hypothesised an association of SDF-1β G801A (rs1801157) and GNB3 C825T (rs5443) single nucleotide polymorphisms (SNPs) with the presence and severity of CAD....
Source: British Journal of Biomedical Science - September 28, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Role of variants rs5030717 and rs5030718 of TLR4 in the risk prediction of nephropathy, hypertension and dyslipidaemia in type 2 diabetes mellitus.
CONCLUSION: TLR4 polymorphisms rs5030717 and rs5030718 may be useful in predicting those type 2 diabetics who are at risk of hypertension, nephropathy and/or dyslipidaemia. PMID: 30211669 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - September 15, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

microRNA-935 is reduced in non-small-cell lung cancer tissue, is linked poor outcome, and acts on signal transduction mediator E2F7 and the AKT pathway.
Authors: Wang C, Li S, Xu J, Niu W, Li S Abstract BACKGROUND: A potential role for microRNA-935 (miR-935) has been identified in several cancers but not in non-small cell lung cancer (NSCLC). We hypothesised changes in miR-935 in NSCLC, and proposed mechanisms that may further explain its role in carcinogenesis. METHODS: NCSLC tissue and nearby normal tissue was obtained from 101 patients and was probed by qRT-PCR for miR-935 expression. The role of miR-935 and a potential target (signal transduction factor E2F7) was determined in cell lines by a dual luciferase assay. The function of miR-935 was investigated t...
Source: British Journal of Biomedical Science - September 13, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of Genetic Polymorphisms of Chemokines and their Receptors with clearance or persistence of hepatitis C virus infection.
Authors: El-Bendary M, Neamatallah M, Elalfy H, Besheer T, El-Setouhy M, Mahfouz M, Zein M, Elhammady D, Hegazy A, Esmat G Abstract BACKGROUND: Polymorphisms of certain genes may have an effect on either persistence of infection or spontaneous clearance of hepatitis C virus (HCV). We hypothesized that one or more variants of chemokines (CCL2 and CCL5) and chemokine receptors (CCR2) genes are associated with the susceptibility to HCV infection. METHODS: We recruited 1460 patients with chronic HCV (CHC), 108 subjects with spontaneous virus clearance (SVC), and 1446 individuals as a healthy control group. All were...
Source: British Journal of Biomedical Science - September 5, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

BPI-ANCA in chronic obstructive pulmonary disease with pulmonary Pseudomonas aeruginosa colonization: a potentially novel indicator for poor prognosis.
Authors: Tian Y, Zeng T, Tan L, Wu Y, Yu J, Huang J, Pei Z PMID: 30105943 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - August 16, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

SNP rs10800708 within the KIF14 miRNA binding site is linked with Breast Cancer.
Authors: Shasttiri A, Rostamian Delavar M, Baghi M, Dehghani Ashkezari M, Ghaedi K PMID: 30092718 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - August 11, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Serum leptin and Homeostasis Model Assessment-IR as novel predictors of early liver fibrosis in chronic hepatitis B virus infection.
Authors: Mousa N, Abdel-Razik A, Sheta T, Shabana W, Zakaria S, Awad M, Abd Elsalam M, El-Wakeel N, Elkashef W, Effat N, Elgamal A, G Deiab A, Eldars W Abstract BACKGROUND: The relationship between hepatitis B virus (HBV) infection, leptin and insulin resistance remains unclear. We hypothesised links between serum leptin and insulin resistance in non-diabetic patients with chronic viral hepatitis B infection and their relation to liver fibrosis. METHODS: We recruited 190 untreated patients with chronic HBV infection and 72 healthy controls. Serum leptin, fasting glucose, insulin, liver function tests (LFTs), C-...
Source: British Journal of Biomedical Science - August 7, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Prognostic significance of soluble major histocompatibility complex class I-related chain A (sMICA) in gastric cancer.
Authors: Xu Y, Chen D, Cheng H Abstract OBJECTIVE: Gastric cancer is a common malignancy, but there are no reliable blood markers for this disease. We tested the hypothesis of a link between serum soluble major histocompatibility complex class I-related chain A (sMICA) and clinicopathological features in gastric cancer. METHODS: A total of 196 patients who underwent gastrectomy and 120 controls (46 healthy individuals, 41 minimal gastritis, 7 gastric ulcers and 26 normal appearance of the gastric mucosa on gastroscopic examination) were enrolled. Serum levels of sMICA were assessed by ELISA. The link between sM...
Source: British Journal of Biomedical Science - July 31, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Monocyte/Granulocyte to Lymphocyte ratio and the MELD score as Prognostic Predictors for Early Recurrence of Hepatocellular Carcinoma after Trans-Arterial Chemoembolization.
Authors: Elalfy H, Besheer T, El-Maksoud MA, Farid K, Elegezy M, El Nakib AM, El Aziz MA, El-Khalek AA, El-Morsy A, Elmokadem A, Elsamanoudy AZ, El-Bendary MA Abstract BACKGROUND: The first-line treatment option for intermediate-stage hepatocellular carcinoma is transarterial chemoembolization. Certain blood indices, such as lymphocyte/monocyte ratio (LMR), lymphocyte count, neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR), monocyte-granulocyte/lymphocyte ratio (MGLR) and red blood cell distribution width (RDW) are prognostic biomarkers in certain diseases. The model for end-stage liver disease (M...
Source: British Journal of Biomedical Science - July 12, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Development of a multiplex two-gene real-time PCR assay for accurate detection of Klebsiella pneumoniae.
CONCLUSION: We report a high sensitive and specific multiplex PCR assay for rapid and accurate diagnosis of K. pneumoniae. PMID: 29991336 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 12, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Molecular characterization of plasmid encoded blaTEM, blaSHV and blaCTX-M among extended spectrum β-lactamases [ESBLs] producing Acinetobacter baumannii.
Molecular characterization of plasmid encoded blaTEM, blaSHV and blaCTX-M among extended spectrum β-lactamases [ESBLs] producing Acinetobacter baumannii. Br J Biomed Sci. 2018 Jul 02; Authors: Girija S, Priyadharsini VJ, Paramasivam A PMID: 29962277 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 4, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A method for improving the accuracy of non-invasive prenatal screening by cell-free foetal DNA size selection.
Conclusion cfDNA size selection is an important step in improving the accuracy of non-invasive prenatal screening for chromosomal abnormalities. PMID: 29968522 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 4, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Influence of single nucleotide polymorphisms in pri-miR-124-1 and STAT3 genes on gastric cancer susceptibility.
Authors: Mirnoori SM, Shahangian SS, Salehi Z, Mashayeki F, Talesh Sasani S, Saedi HS Abstract INTRODUCTION: MicroRNAs (miRNAs) are small ribonucleic acids that modulate the expression of downstream target genes. There is considerable evidence of their involvement in many malignancies, such as oesophageal and gastric. We hypothesised altered expressions of pri-miR-124-1 rs531564 and STAT3 rs1053023 polymorphisms in gastric cancer. MATERIALS AND METHODS: Genomic DNA was extracted from peripheral blood of 250 patients with gastric cancer and 310 healthy individuals. The RFLP method was applied for determination o...
Source: British Journal of Biomedical Science - June 28, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Impact of Non-alcoholic fatty liver disease on pregnancy.
CONCLUSION: Pregnant women with NAFLD have increased risk for GDM, pre-eclampsia, hypertension, hyperuricemia, increasing total cholesterol and triglycerides and should be carefully monitored during antenatal care.b. PMID: 29943682 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 28, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The association of single nucleotide polymorphisms of Toll-like receptor 3, Toll-like receptor 7 and Toll-like receptor 8 genes with the susceptibility to HCV infection.
Authors: El-Bendary M, Neamatallah M, Elalfy H, Besheer T, Elkholy A, El-Diasty M, Elsareef M, Zahran M, El-Aarag B, Gomaa A, Elhammady D, El-Setouhy M, Hegazy A, Esmat G Abstract BACKGROUND: Single nucleotide polymorphisms (SNPs) of Toll-like receptors (TLRs) have been associated with functional modification or impairment of cytokine responses. In chronic hepatitis C virus (HCV) infection, studies of TLR polymorphisms have primarily targeted receptor pathways implicated in viral immune responses. We hypothesized that one or more variant (s) of TLR3, TLR7 and TLR8 are associated with different outcomes of HCV infec...
Source: British Journal of Biomedical Science - June 28, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

IL-28B rs12979860 polymorphism affect the course of chronic hepatitis and the development of HCC in Egyptian patients with hepatitis C type 4.
Authors: Attallah AM, Omran D, Marie MS, Abdelrazek MA, Salama A, El Essawey R, Mobarak L, Maklad S, Omar A Abstract BACKGROUND: A single nucleotide polymorphism (SNP) in the interleukin 28B (IL28B) gene may alter the trajectory of hepatitis C virus (HCV) chronic infection. Several studies have sought to determine a link between the IL28B rs12979860 SNP and the development of HCV-related hepatocellular carcinoma (HCC), but with variable results, consensus is awaited. We hypothesised that IL28B rs12979860 SNP is linked to HCC in patients with HCV type 4. METHODS: IL28B genotyping of 300 patients with HCV related...
Source: British Journal of Biomedical Science - June 21, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Impact of GSTM1, GSTT1 and GSTP1 genes polymorphisms on clinical toxicities and response to concomitant chemoradiotherapy in cervical cancer.
CONCLUSIONS: Although no significant links were found between GST polymorphism and treatment response, null genotypes of GSTM1, GSTT1 and 'G' allele of GSTP1 bring a higher risk of severe gastrointestinal toxicity due to chemoradiation therapy in cervical cancer. PMID: 29909733 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 20, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The gamma-glutamyl transferase to platelet ratio and the FIB-4 score are noninvasive markers to determine the severity of liver fibrosis in chronic hepatitis B infection.
Conclusions FIB-4 and GPR may be useful blood markers for evaluating the severity of liver fibrosis in chronic hepatitis B patients. Further prospective study is required to validate these noninvasive blood markers in a clinical practice. PMID: 29893189 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 14, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Genetic polymorphisms in KCNJ11 (E23K, rs5219) and SDF-1 β (G801A, rs1801157) genes are associated with the risk of type 2 diabetes mellitus.
Genetic polymorphisms in KCNJ11 (E23K, rs5219) and SDF-1β (G801A, rs1801157) genes are associated with the risk of type 2 diabetes mellitus. Br J Biomed Sci. 2018 Jun 12;:1-6 Authors: Rizvi S, Raza ST, Mahdi F, Singh SP, Rajput M, Rahman Q Abstract Background Type 2 diabetes mellitus (T2DM) is a global major health problem resulting from interaction of environmental and genetic factors, examples of the latter being KCNJ11 (coding for part of the ATP-sensitive potassium channel) and SDF-1β (coding for chemokine CXCL12). Our case-control study was conducted to assess whether recessive, dominant...
Source: British Journal of Biomedical Science - June 14, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Clinical value of a diagnostic score for colon cancer based on serum CEA, CA19-9, cytokeratin-1 and mucin-1.
Authors: Attallah AM, El-Far M, Ibrahim AR, El-Desouky MA, Omran MM, Elbendary MS, Attallah KA, Qura ER, Abdallah SO Abstract BACKGROUND: Although established markers such as CEA and CA19-9 are important for diagnosing early stages of colon cancer, they are not ideal. Developing promising markers include cytokeratin 1 (CK1) and mucin-1 (MUC1), but the combined value of each of these markers is unclear. We therefore evaluated the value of a combined laboratory-based score of these four markers in the diagnosis of colon cancer. METHODS: Two hundred patients who had undergone colonoscopic examination (150 colon ca...
Source: British Journal of Biomedical Science - May 9, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Haematoxylin - the story of the blues.
Authors: Orchard GE PMID: 29668360 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - April 19, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The role of rs267606943 polymorphism in the prolidase gene and plasma prolidase in polycystic ovary syndrome.
Authors: Bhatnager R, Nanda S, Dang AS PMID: 29668362 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - April 19, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Rapid detection and subtyping of human papillomaviruses in condyloma acuminatum using loop-mediated isothermal amplification with hydroxynaphthol blue dye.
Conclusion The results of this study indicated that the LAMP assay with HNB is superior to the Luminex method in terms of sensitivity and specificity. The specificity of LAMP was 100% and the sensitivity of LAMP was 1000 copies/tube using HNB. LAMP is therefore a useful, quick and accurate method for the clinical diagnosis of HPV subtypes. PMID: 29537347 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - March 18, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The relationship of systemic markers of haemostasis with retinal blood vessel responses in cardiovascular disease and/or diabetes.
Conclusions In addition to glycaemia, several haemostasis measures, but no measures of platelet activity, are linked to ocular and retinal blood vessel indices in patients with diabetes and/or cardiovascular disease. These associations may have pathophysiological significance. PMID: 29521170 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - March 10, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Effects of miR-27a, miR-196a2 and miR-146a polymorphisms on the risk of breast cancer.
Authors: Mashayekhi S, Saeidi Saedi H, Salehi Z, Soltanipour S, Mirzajani E Abstract Background microRNAs (miRNAs) are potentially involved in many physiopathological processes, including regulation of cell growth, differentiation, apoptosis and cancer. Single nucleotide polymorphisms of the genes encoding miRNAs can alter their expression and may influence cancer risks. This case-control study explored the relationship between three microRNA polymorphisms (miR-27a, miR-196a2 and miR -146a) and breast cancer (BC). Methods A total of 353 breast cancer cases and 353 controls were genotyped for miR-27a (rs895819), miR...
Source: British Journal of Biomedical Science - March 10, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Serum relaxin-2 as a novel biomarker for prostate cancer.
Authors: Xu Y, Yu Q, Liu Y PMID: 29513121 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - March 9, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Belfast Agar-a simple laboratory medium to separate Pseudomonas aeruginosa from pan-resistant Burkholderia cenocepacia isolated from the sputum of patients with cystic fibrosis (CF).
Authors: Caskey S, Moore JE, McCaughan J, Rendall JC PMID: 29513134 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - March 9, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Prognostic value of serum levels of soluble MICA (sMICA) in patients with prostate cancer.
Authors: Liu Y, Guo X, Xing M, Zhao K, Luo L, Du J PMID: 29513160 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - March 9, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Functional variants of p21 gene alter susceptibility to meningioma.
Authors: Mashayekhi S, Salehi Z, Saberi A, Shakiba M, Mashayekhi F, Yousefzadeh-Chabok S PMID: 29457958 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 21, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Examination of 16S-23S rRNA intergenic spacer region (ISR) heterogeneity in a population of clinical Streptococcus pneumoniae- a new laboratory epidemiological genotyping tool to aid outbreak analysis.
Authors: Moore JE, Hirayama J, Hayashi K, Mason C, Coulter W, Matsuda M, Goldsmith CE PMID: 29457961 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 21, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Serum CA125 in combination with ferritin improves diagnostic accuracy for epithelial ovarian cancer.
Authors: Zhao J, Guo N, Zhang L, Wang L Abstract INTRODUCTION: CA125 has poor sensitivity and low specificity for detecting early ovarian cancer. Serum ferritin is elevated in many malignancies. We evaluated the performance of ferritin alone and in combination with CA125 as a diagnostic tool to detect epithelial ovarian cancer (EOC). METHODS: CA125 and ferritin were detected in the serum of 50 healthy control (HC), 50 women with benign gynaecological conditions and 124 women with EOC. The relationship between serum ferritin and CA125 and each of the clinicopathological parameters was assessed, and their diagnos...
Source: British Journal of Biomedical Science - February 18, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Can combined blood neutrophil to lymphocyte ratio and C-reactive protein be used for diagnosis of spontaneous bacterial peritonitis?
CONCLUSIONS: Combined NLR and CRP could be used as a novel, simple, low-cost, non-invasive test for SBP diagnosis. PMID: 29452544 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 18, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Potential value of circulating microRNA-126 and microRNA-210 as biomarkers for type 2 diabetes with coronary artery disease.
Authors: Amr KS, Abdelmawgoud H, Ali ZY, Shehata S, Raslan HM Abstract BACKGROUND: Macrovascular complications are the main cause of morbidity and mortality among the diabetic patients. MicroRNAs (miRNAs), a family of small non-coding RNAs, play vital roles in the regulation of blood glucose level and the concurrent cardiovascular complications of type 2 diabetes. We hypothesized that plasma miR-126 and miR-210 are linked to coronary artery disease (CAD) in these diabetes patients. METHODS: Fasting blood samples were collected from 20 healthy volunteers and 100 patients with diabetes (54 patients without CAD an...
Source: British Journal of Biomedical Science - February 18, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of Helicobacter pylori and protozoal parasites in patients with chronic diarrhoea.
Authors: Yakoob J, Abbas Z, Khan R, Tariq K, Awan S, Beg MA Abstract Introduction An association of Helicobacter pylori and common protozoal parasites in patients with abdominal discomfort and chronic diarrhoea is unclear and may be pathological. Materials and methods One hundred and sixty-one patients with diarrhoea were compared to 114 age and sex matched controls. Stool samples were examined by microscopy and DNA extracted for PCR with specific primers for H. pylori and protozoal parasites Blastocystis sp., Entamoeba sp. (Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii) and Giardia duodenalis (...
Source: British Journal of Biomedical Science - February 18, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Book Reviews.
Authors: Verran J, Smith RF PMID: 29443658 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 17, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Leptin stimulates the proliferation of human oesophageal adenocarcinoma cells via HB-EGF and TGF α mediated transactivation of the epidermal growth factor receptor.
This study examines the effect of leptin on EGFR signalling in cultured cell lines. Leptin stimulated proliferation in four OAC lines expressing leptin receptors (OE33, OE19, BIC-1 and FLO) and this was abolished by specific EGFR inhibitors (PD153035 and AG1478). Leptin-induced proliferation was inhibited by neutralising antibodies to transforming growth factor-α (TGFα and HB-EGF) but not by anti-amphiregulin. Leptin significantly increased gene expression of HB-EGF and TGFα as measured by a quantitative real-time polymerase chain reaction (PCR) method but did not alter amphiregulin and EGFR gene expressi...
Source: British Journal of Biomedical Science - February 17, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Antagonistic interactions between the flavonoids hesperetin and naringenin and β-lactam antibiotics against Staphylococcus aureus.
This study shows that the flavonoids hesperetin and naringenin have antibacterial activity against methicillin-sensitive and methicillin-resistant Staphylococcus aureus isolates. Minimum inhibitory concentrations for hesperetin were 250 and 500 g/mL, respectively, and for naringenin were 125 and 250 g/mL, respectively. This effect was reversed by the -lactam antibiotics methicillin, penicillin and oxacillin, but not by cefoxitin. For bacteria growing in the presence of these antibiotics, the flavonoids had no effect on the levels of -lactamase enzymes and PBP-2' compared to controls. Electron microscopy showed abnormal mor...
Source: British Journal of Biomedical Science - February 17, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Book Reviews.
Authors: Dawson M PMID: 29443661 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 17, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Clinical and epidemiological characterisation of Burkitt's lymphoma: an eight-year case study at Komfo Anokye Teaching Hospital, Ghana.
Authors: Owusu L, Yeboah FA, Osei-Akoto A, Rettig T, Arthur FKN PMID: 29443678 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 17, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Book Reviews.
Authors: Shanks D, Wang Q, Verran J, Harris E PMID: 29443679 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 17, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Serum procalcitonin, interleukin-6, soluble intercellular adhesin molecule-1 and IgG to shortchain exocellular lipoteichoic acid as predictors of infection in total joint prosthesis revision.
Authors: Worthington T, Dunlop D, Casey A, Lambert P, Luscombe J, Elliott T Abstract The diagnosis of prosthetic joint infection and its differentiation from aseptic loosening remains problematic. The definitive laboratory diagnostic test is the recovery of identical infectious agents from multiple intraoperative tissue samples; however, interpretation of positive cultures is often complex as infection is frequently associated with low numbers of commensal microorganisms, in particular the coagulase-negative staphylococci (CNS). In this investigation, the value of serum procalcitonin (PCT), interleukin-6 (IL-6) and...
Source: British Journal of Biomedical Science - February 17, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Book Reviews.
Authors: Verran J, Hughes NT, Orchard G, Watson P PMID: 29443681 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 17, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Non-coding small (micro) RNAs of Pseudomonas aeruginosa isolated from clinical isolates from adult patients with cystic fibrosis.
This report presents data on these unknown cellular miRNAs cloned from P. aeruginosa isolates from CF patients. Adapting a computational miRNA prediction model that takes advantage of the highly conserved known miRNA hair pin stems regions, the results revealed that the fold structure of the microRNAs had a high homology to the recently reported human bacterial infection response (BiR)-related microRNA, mi-146, associated with the Toll-like receptor (TLR) family, which is the primary evolutionarily conserved sensors of pathogen-associated molecular patterns (PAMPs), and known to trigger host inflammatory and immune respons...
Source: British Journal of Biomedical Science - February 17, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Book Reviews.
Authors: Gaffney J PMID: 29443683 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 17, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Book Reviews.
Authors: Flannagan R, Heath M, Watson P, Wilkinson M, Mera SL, Overfield JA, Edwards-Jones V, McLellan DS PMID: 29443684 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 17, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Moments of Truth: Four Creators of Modern Medicine.
Authors: Scott TG PMID: 29443685 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 17, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Data Analysis and Presentational Skills.
Authors: Scott TG PMID: 29443686 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 17, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A Beginner's Guide to Blood Cells.
Authors: Macey MG PMID: 29443687 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 17, 2018 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research