The effects of human immunodeficiency virus, human papillomavirus, herpes simplex virus-1 and -2, human herpesvirus-6 and -8, cytomegalovirus, and hepatitis B and C virus on female fertility and pregnancy.
We present an overview of the impact of common viral infections on female reproduction, searching Medline, PubMed, Scopus, and Google scholar databases to find the potentially relevant studies of viruses known to have a potential effect. Viruses such as human immunodeficiency virus (HIV), herpes simplex virus (HSV) and human herpesvirus (HHV) increased infertility rates whilst human papillomavirus (HPV), cytomegalovirus (CMV), and hepatitis B and C virus (HBV, HCV) infections mostly led to higher rates of abortion and miscarriage. Moreover, HPV infection is linked to increased tubal infertility, endometriosis, and pelvic i...
Source: British Journal of Biomedical Science - July 31, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Efficacy of laparoscopic gastric bypass vs laparoscopic sleeve gastrectomy in treating obesity combined with type-2 diabetes.
Authors: Yan Y, Wang F, Chen H, Zhao X, Yin D, Hui Y, Ma N, Yang C, Zheng Z, Zhang T, Xu N, Wang G Abstract OBJECTIVE: This prospective study compared the efficacy and safety of laparoscopic gastric bypass and laparoscopic sleeve gastrectomy in treating overweight and obese patients with BMI>28 kg/m2 and type-2 diabetes. METHODS: Patients were randomized into a gastric bypass group (n=77) or a gastrectomy group (n=80). The surgery time, intraoperative blood loss, recovery time, and hospitalization time were collected. BMI, waistline, hipline, C-peptide level, insulin resistance index (HOMA-IR), and their blo...
Source: British Journal of Biomedical Science - July 24, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The relationships between maternal and placental polymorphisms of miR-196a2 and miRNA-499 genes and preeclampsia.
CONCLUSION: The placental but not maternal miRNA-196a2 rs11614913 variant could be a protective factor for preeclampsia predisposition in all models except the recessive model. The maternal/placental rs3746444 CC genotype was in association with higher preeclampsia risk. PMID: 32686997 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 21, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Overexpression of a Novel Long Non-Coding RNA MCM3AP-AS1 in breast cancer tissues compared to adjacent non-tumour tissues.
Authors: Riahi A, Hosseinpour-Feizi M, Rajabi A, Akbarzadeh M, Montazeri V, Safaralizadeh R Abstract BACKGROUND: Altered expression of several long non-coding RNAs (lncRNAs) have been described in numerous malignancies, including breast cancer, and some may have a role in carcinogenesis. We hypothesised differences in the expression of lncRNA MCM3AP-AS1 in breast cancer tissues compared to nearby healthy tissues and potential links with clinical features. METHODS: We tested our hypothesis in 102 pairs of breast cancer tumours and adjacent non-tumour tissues from female patients. After RNA extraction, cDNA synth...
Source: British Journal of Biomedical Science - July 19, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Serum long noncoding RNAs FAS-AS1 & PVT1 are novel biomarkers for systemic lupus erythematous.
Serum long noncoding RNAs FAS-AS1 & PVT1 are novel biomarkers for systemic lupus erythematous. Br J Biomed Sci. 2020 Jul 02;:1-5 Authors: Ali MA, Shaker OG, Khalefa AA, Abdelwahed MY, Ali E, Ezzat EM, Elghobary HA, Awaji AA, Fouad NA, Ayoub SE Abstract BACKGROUND: Systemic Lupus Erythematous (SLE) is a chronic systemic autoimmune disorder whose diagnosis depends on combination of multiple factors. Circulating lncRNAs could serve as diagnostic non-invasive biomarkers for SLE. We hypothesised that serum FAS-AS1 and PVT1 are new biomarkers for SLE that relate to clinical features and laboratory marker...
Source: British Journal of Biomedical Science - July 4, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association between SLCO1B1 polymorphism distribution frequency and blood lipid level in Chinese adults.
Authors: Mo Q, Huang S, Ma J, Zhang J, Su R, Deng Q Abstract BACKGROUND: The variation of serum lipid levels can be part-related to certain genes. One such gene, SLCO1B1, encodes a transporter that may have a role in lipid metabolism. We hypothesised that differences in certain SLCO1B1 genotypes are related to levels of serum lipids. MATERIALS AND METHODS: We recruited genotyped 636 subjects who were genotyped for SLCO1B1 variants *1a, *1b, *5 and *15. Routine liver function tests, renal function tests and routine lipid indices were measured by standard techniques. RESULTS: The most frequent genotypes were ...
Source: British Journal of Biomedical Science - July 1, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of miR-146a rs57095329 with Behcet's disease and its complications.
Authors: Abdelaleem OO, Fouad NA, Shaker OG, Hussein HA, Ahmed FA, Ali DY, Elsayed HS Abstract BACKGROUND: Behçet's disease is a chronic relapsing and remitting autoimmune multisystem inflammatory disease characterised by oral aphthae, genital ulcers, skin lesions, gastrointestinal involvement, arthritis, vascular lesions, and neurological manifestations. We hypothesised a link between rs57095329 of miR-146a and Behçet's disease, with further links with common clinical features. METHODS: We tested our hypothesis in 130 Behçet's disease patients and 131 age and sex-matched healthy controls. ...
Source: British Journal of Biomedical Science - June 24, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Toll-like receptor 7 mRNA is reduced in hepatitis C based liver cirrhosis and hepatocellular carcinoma, out-performs alpha-feto protein levels, and with age and serum aspartate aminotransferase is a new diagnostic index.
Authors: Mohamed AA, Omran D, El-Feky S, Darwish H, Kassas A, Farouk A, Ezzat O, Abdo SM, Zahran FE, El-Demery A, Omran MM Abstract BACKGROUND: Hepatitis B and C viruses are leading causes of liver cirrhosis and hepatocellular carcinoma (HCC). Toll-like receptor 7 (TLR7) has been implicated in the pathogenesis of HCC linked to hepatitis B. We hypothesised a role of leukocyte TLR7 mRNA in hepatitis C related liver cirrhosis and HCC, using alpha fetoprotein (AFP) and liver function tests as comparators. METHODS: We recruited 102 patients with HCV-related HCC, 97 with HCV related liver cirrhosis and 60 healthy con...
Source: British Journal of Biomedical Science - June 24, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Atypical and novel presentations of Coronavirus Disease 2019: a case series of three children.
We report three children whose initial presentation was gastrointestinal, and in whom COVID-19 infection was found, concluding that cases of acute appendicitis, mesenteric adenitis and flank tenderness may mask and infection with this virus, which should therefore be investigated. PMID: 32552415 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 20, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A novel serum metabolome score for breast cancer diagnosis.
Authors: Rashed R, Darwish H, Omran M, Belal A, Zahran F Abstract INTRODUCTION: Early detection of breast cancer is helpful in the diagnosis and treatment, and so in enhancing patient survival and reducing death rates. Because of the low diagnostic sensitivity and specificity of widely used breast cancer biomarkers such as CA15-3, we hypothesised that a panel of new metabolic markers would provide superior sensitivity and specificity for this disease. MATERIAL & METHODS: We recruited 120 women cases with malignant breast cancer, 47 with benign breast disease and 55 females as a healthy control group. Metabo...
Source: British Journal of Biomedical Science - June 19, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Haplotype-based association study of Opioid Receptor Kappa-type 1 (OPRK1) gene polymorphisms with nicotine dependence among male smokers.
Authors: Albonaim A, Sharafshah A, Keshavarz P PMID: 32508243 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 10, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Fine structure of the human retina defined by confocal microscopic immunohistochemistry.
CONCLUSION: The multiple labelling of human retinal cells brings further understand of the biological characteristics and functions of these cells, and provides a theoretical basis for their possible role in diseases. In the growing field of human retina research, our data may provide a point of reference for future studies of the human retina. PMID: 32498649 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 7, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Epidermal growth factor +61A/G (rs4444903) promoter polymorphism and serum levels are linked to idiopathic male infertility.
Authors: Malek MA, Mashayekhi F, Salehi Z PMID: 32448090 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 27, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Circulating Micro RNA-223 and Angiopoietin-Like Protein 8 as Biomarkers of Gestational Diabetes mellitus.
Authors: Abdeltawab A, Zaki MES, Abd El Dayem Y, Mohamed AA, Abbas S Abstract Background: Gestational diabetes mellitus (GDM) is a serious health problem associated with both fetal and maternal complications. New biomarkers that can predict or help in the early diagnosis of GDM are needed to minimize the hazards of hyperglycemia on pregnant women and their offspring. We hypothesised a link between levels of microRNA-223 (miRNA-223) and Angiopoietin-Like Protein 8 (ANGPTL8) and GDM.Materials and Methods: The study included 109 patients with confirmed early diagnosed GDM and 103 healthy control pregnant women in thei...
Source: British Journal of Biomedical Science - May 20, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of hTERT SNP (rs2736100) with implantation failure after in vitro fertilization and embryo transfer (IVF-ET).
Authors: Tajalli S, Mashayekhi F, Salehi Z, Arefi S, Sasani ST PMID: 32401182 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 15, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of Estrogen Receptor Alpha gene SNPs Arg157Ter C > T and Val364Glu with Female Infertility.
Association of Estrogen Receptor Alpha gene SNPs Arg157Ter C>T and Val364Glu with Female Infertility. Br J Biomed Sci. 2020 Apr 21; Authors: Zaer SJ, Farsimadan M, Abiri A, Sharafshah A, Vaziri H PMID: 32316865 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - April 24, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Nicotinic acetylcholine receptors (rs1051730) gene polymorphism and Surfactant protein D level in chronic obstructive pulmonary disease.
Authors: El Gayed EA, Elhelbawy RH, Elhelbawy NG Abstract Chronic obstructive pulmonary disease (COPD) is a complicated disorder that may be genetically determined. It is possible that genetic determinants of nicotine addiction contribute to COPD burden. Surfactant protein D (SP-D) ensures correct ventilatory function . The aim of this work is to study serum level of (SP-D) and nicotinic acetylcholine receptors (nAChRs) (rs1051730) (G/A) gene polymorphism in COPD. We recruited 80 patients with COPD and 80 ages and gender matched healthy individuals. Estimation of Serum SP-D level (by ELISA) and genotyping of (nAChR...
Source: British Journal of Biomedical Science - April 18, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Prognostic value of bone marrow MUC4 expression in acute myeloid leukemia.
CONCLUSION: MUC4 over-expression is an independent predictor of a poor prognosis in AML patients. PMID: 32270747 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - April 11, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Demonstration of microRNA using in situ hybridisation on formalin fixed paraffin wax samples using conventional oligonucleotide probes: A comparison with the use of locked nucleic acid probes.
Conclusion: This study has shown that simplification of ISH protocols by the use of COPs provides equivalent results to the use of LNA methods and it can be used to precisely identify cells in which miRNAs are expressed. PMID: 32223721 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - April 1, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Polymorphisms rs763110 in FASL is linked to hepatitis C virus infection among high-risk populations.
Authors: Huang P, Wang C, Zhuo L, Xia X, Yang S, Zhang J, Fan H, Wu J, Bin Y, Yue M, Zhang Y Abstract Background: The Fas cell surface death receptor (FAS) and Fas ligand (FASL) can participate in the apoptosis of immune cells and target cells infected with a virus through the FAS-FASL signaling pathway. The decoy receptor 3 (DCR3) can competitively inhibit the binding of FAS to FASL. Our aim is to investigate the effect of single nucleotide polymorphisms (SNPs) in FAS, FASL and DCR3 on hepatitis C virus (HCV) infection.Methods: Four SNPs (rs763110 in FASL, rs1324551 and rs2234767 in FAS and rs2257440 in DCR3) were...
Source: British Journal of Biomedical Science - March 28, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Serum LINC00305 expression and its genetic variant rs2850711 are associated with clinical and laboratory features of rheumatoid arthritis.
Authors: Wahba AS, Ibrahim ME, Mesbah NM, Saleh SM, Abo-Elmatty DM, Mehanna ET Abstract Background: Long intergenic non-Protein coding (lnc) RNA 00305 (LINC00305) is a pro-inflammatory atherosclerosis-associated lncRNA. We hypothesised altered LINC00305 variant rs2850711 (A/T) in rheumatoid arthritis (RA) patients and links with clinical and routine laboratory markers.Methods: 100 RA patients and 100 healthy controls were recruited. LINC00305 genotyping and expression were performed using allelic-discrimination PCR and quantitative real-time PCR. LINC00305 diagnostic power was evaluated using area under the receive...
Source: British Journal of Biomedical Science - March 21, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

PADI4 (rs2240340), PDCD1 (rs10204525), and CTLA4 (231775) Gene Polymorphisms and Polyarticular Juvenile Idiopathic Arthritis.
Authors: Ali M, Kaddafy S, Abdelaziz A, Abonar A, Hanafy M, Hussein H, Shabana H, Abd El-Hmid R, Ali M Abstract Background: Certain single nucleotide polymorphisms (SNPs) in genes such as PAD14 (coding for peptidyl arginine deiminase 4), PDCD1 (coding for programmed cell death 1), and CTLA4 (coding for cytotoxic T-lymphocyte-associated protein 4) are linked to rheumatoid arthritis (RA). However, links between SNPs rs2240340, rs10204525 and rs231775 in PAD14, PDCD1 and CTLA4 respectively, and juvenile idiopathic arthritis (JIA), the commonest type of childhood arthritis are unclear. We aimed to determine whether any...
Source: British Journal of Biomedical Science - March 13, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The Effect of CASP3 rs4647610 and rs4647602 Polymorphisms on Tumour Size and Cancer Stage in Papillary Thyroid Carcinoma.
Conclusion: Although CASP3 rs4647610 and rs4647602 SNPs are not associated with PTC, rs4647610 is linked to larger tumour size, and rs4647602 to lower stage of cancer. PMID: 32151194 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - March 12, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Reduced Sperm Concentration in a Patient from a Suspected Post-Operative Infection: a case study.
Authors: Long S, Dawe S, Woodward B Abstract A diagnostic semen analysis should be performed as part of a couple's routine fertility investigations in order to determine sperm quality prior to managing the treatment pathway. The semen analysis report should be considered alongside clinical discussions and a review of both patients' medical history. However, whilst it is part of the standard patient pathway, a regular up-to-date review at each clinical step of a patients' journey is not always performed, which may miss potential clinical changes that could impact the most effective management of the couple. This cas...
Source: British Journal of Biomedical Science - February 25, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A comparison of the HAIN Genotype CM reverse hybridisation assay with the Bruker MicroFlex LT MALDI-tof mass spectrometer for identification of clinically relevant mycobacterial species.
ConclusionsThis comparative study has shown that the use of MALDI-tof for identification of Mycobacteria has resulted in a reduction in turnaround times, a dramatic reduction in the cost of identification and less preparation time in the laboratory. Overall, the results show that MALDI-tof appears to be a suitable replacement for the Genotype CM assay in the routine clinical Mycobacteriology laboratory and the methods described herein can act as a roadmap for other laboratories to implement the technology successfully. PMID: 32072855 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 21, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Upregulation of miR-410 is linked with unfavorable prognosis in patients with colorectal cancer.
Authors: Abedi P, Bayat A, Ghasemzadeh S, Raad M, Pashaiefar H, Ahmadvand M Abstract Background: Although miR-410 is reported to act as a cancer inducer in colorectal cancer, there is limited data on the clinical implications of miR-410 expression levels in patients. We hypothesised a link between miR-410 expression and its potential clinical values in patients with colorectal cancer.Material and methods: 120 colorectal cancer tissue specimens and 120 adjacent non-tumor tissues were obtained. Quantification of miR-410 expression levels was determined by, quantitative RT-PCR. Expression was analysed by clinical feat...
Source: British Journal of Biomedical Science - February 20, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR.
Authors: Sánchez AG, Juaneda I, Eynard H, Basquiera AL, Palazzo E, Calafat P, Palla V, Romagnoli PA, Alvarellos T Abstract Background: CAT25 (T25mononucleotide repeat of the Caspase 2 gene), is a promising DNA marker for detecting microsatellite instability (MSI) in colorectal cancer. CAT25 has the potential to be incorporated into the Bethesda panel, a commonly used panel of DNA microsatellites, or replace it in its entirety. We aimed to develop and validate a high-resolution melting-PCR (HRM-PCR) method for CAT25 instability detection in clinical samples and study its allelic variation in our population.Me...
Source: British Journal of Biomedical Science - February 20, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Relevance of KISS1 gene polymorphisms in susceptibility to Polycystic Ovary Syndrome and its associated Endocrine and Metabolic disturbances.
Conclusion: Women having rs1213704663C>G variation in KISS1 gene are highly susceptible to PCOS and its associated endocrine and metabolic disturbances (LH and estradiol hypersecretion, and increased LH/FSH).Keywords: Polycystic ovary syndrome, gene polymorphisms, Metabolic and Endocrine disturbances, LH, estradiol. PMID: 32031919 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 8, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of rs2620381 polymorphism in miR-627 and gastric cancer.
Conclusion: Presence of the C SNP in miR-627 rs2620381 in linked with gastric cancer, and may be important in pathogenesis. PMID: 31983284 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 29, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Malondialdehyde and C-Reactive Protein as prognostic markers of Hepatocellular Carcinoma.
Authors: S E, Naser M, Tarek B, Tarek S, Mahmoud A, K T, Narmin E, A E, A AR Abstract Background . Hepatocellular carcinoma (HCC) is a common cause of cancer-related mortality. The aim of this study is to evaluate the serum Malondialdehyde (MDA) and C-Reactive Protein (CRP) as prognostic markers among patients with HCC and its correlation with the tumor size, Child-Pugh and MELD score.Methods: The study was carried out on 180 patients with HCC and 180 cirrhotic controls. The patients were classified according to the Child-Pugh classification into Child A (n=50), B (n=62) and C (n=68), according to the MELD score in...
Source: British Journal of Biomedical Science - January 25, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Improving the processing time for the detection of carbapenemase-producing enterobacterales using an evolving algorithm.
Authors: Cafferkey J, O'Connor M, Doyle D, Skally M, Fitzpatrick M, Burns K, O'Connell K, Fitzpatrick F, Smyth E, Humphreys H PMID: 31971872 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 25, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Case report: Xanthogranulomatous pyelonephritis masquerading as cystic renal cell carcinoma.
Conclusion: XGP is a diagnostic and therapeutic dilemma. Partial Nephrectomy may be appropriate in management of XGP in select cases. PMID: 31976820 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 25, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Cytokine gene variants and treatment outcome of cisplatin based concomitant chemoradiotherapy in cervical cancer.
Conclusion: Certain cytokine gene variants may help detect susceptibiliy to cervical cancer, and predict response to chemoradiotherapy. PMID: 31935340 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 15, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

TNFSF15 (rs3810936) in Beh çet's disease.
This study aimed to study TNFSF15 (rs3810936) polymorphism distribution, as well as the peripheral blood TNFSF15 expression in BD patients.Methods: This study included 140 healthy controls and 70 BD patients. TNFSF15 gene polymorphism was detected by PCR restriction fragment length polymorphism analysis. The relative expression of TNFSF15 gene was studied by quantitative real-time PCR .Results: BD patients had frequencies of the rs310936 T allele and TT genotype lower than those of the controls (p = 0.037 and 0.033, respectively). TNFSF15 messenger RNA expression in BD patients revealed a significant difference between different genotypes (p
Source: British Journal of Biomedical Science - January 8, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Bilateral spontaneous renal artery dissection and antiphospholipid antibodies.
Authors: Del Porto F, Cifani N, Orgera G, Taurino M, Proietta M Abstract Spontaneous renal artery dissection (SRAD) is a rare disorder with a prevalence of 0.005%, whilst bilateral SRAD is even less frequent. It occurs in about 12-18% of all cases of renal artery dissection and represents a variant entity, whose causes often remain unknown. To our knowledge, only 29 cases of bilateral SRAD have been previously described. Here we report the 30th case of a 46-years-old man with bilateral SRAD dissection and repeatedly positive IgM anticardiolipin antibodies at medium titres that may be pathogenic. PMID: 31903851 ...
Source: British Journal of Biomedical Science - January 8, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Diagnostic role of collagen-III and matrix metalloproteinase-1 for early detection of hepatocellular carcinoma.
Authors: Attallah AM, Albannan MS, El-Deen MS, Farid K, Khedr FM, Attallah KA, Abdallah SO Abstract Background: As the poor prognosis of hepatocellular carcinoma (HCC) is mostly due to late detection at an advanced stage there is a strong need for establishing more effective strategies for early identification. We hypothesised that collagen-III and matrix metalloproteinase-1 (MMP-1) and their ratio (CMR) are effective markers for identifying early-HCC when used alongside serum AFP, alkaline phosphatase and bilirubin.Methods: We recruited 148 patients with HCC, 133 with cirrhosis and 121 with fibrosis. Liver fibrosi...
Source: British Journal of Biomedical Science - January 8, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

British Journal of Biomedical Science in 2019. What have we learned?
Authors: Blann A Abstract In 2019 the British Journal of Biomedical Science published 40 articles in the various disciplines that comprise biomedical science. These were one review, 22 original articles and 17 'In Brief' short reports. Of those citing original data, the majority were in cellular pathology (14 papers), clinical chemistry (9 papers), and microbiology (6 papers: 4 in bacteriology and 2 in virology). There were 3 papers in haematology and 2 in andrology, whilst 5 papers crossed traditional discipline boundaries (such as the molecular genetics of IL6, liver function tests, and hepatocellular carcinoma)....
Source: British Journal of Biomedical Science - December 12, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Methylation of tumour suppressor genes RUNX3, RASSF1A and E-Cadherin in HCV-related liver cirrhosis and hepatocellular carcinoma.
Authors: El-Bendary M, Nour D, Arafa M, Neamatallah M Abstract Background: HCV infection is related to aberrant methylation of several genes. RASSF1A, E-Cadherin and RUNX3 are tumour suppressor genes that may be inactivated by hypermethylation in many tumours including hepatocellular carcinoma (HCC). We hypothesized that methylation is a diagnostic biomarker for HCC in patients with HCV-related liver cirrhosis.Methods: We recruited 207 cases of HCV-related liver cirrhosis, 193 HCC patients and 53 healthy controls. Methylation-specific polymerase chain reaction for detection of circulating hypermethylated RASSF1A, E...
Source: British Journal of Biomedical Science - December 5, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Synergic effects of cancer stem cells markers, CD44 and embryonic stem cell transcription factor Nanog, on bladder cancer prognosis.
Authors: Siddiqui Z, Srivastava AN, Sankhwar SN, Dalela D, Singh V, Zaidi N, Fatima N, Bano I, Anjum S Abstract Purpose of the study: Therapy that targets cancer stem cells has the potential to eradicate cancer cells and prevent tumour recurrence. Therefore, we hypothesised the combined prognostic significance of stem cell markers CD44 (prevalent in basal layer of urothelial carcinoma) and Nanog (embryonic stem cell transcription factor) in bladder cancer.Material and Methods: CD44 and Nanog expression were determined by immunohistochemistry in 112 bladder cancer cases of which 79 were non-muscle invasive and 33 mu...
Source: British Journal of Biomedical Science - November 15, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Apa1 (rs7975232) SNP in the vitamin D receptor is linked to hepatocellular carcinoma in hepatitis C virus cirrhosis.
Authors: Raafat Rowida I, Eshra KA, El-Sharaby RM, Eissa R, Saied SM, Amer I, El Sharawy S Abstract BACKGROUND: As hepatocellular carcinoma (HCC) arising from chronic hepatitis C virus (HCV) infection in liver cirrhosis is a major problem in public health, early and rapid prediction of HCC is urgent. We hypothesised that a single nucleotide polymorphism in the Apa1 SNP in the vitamin D receptor may help diagnosis. METHODS: We recruited 3 groups: 80 HCC patients with HCV cirrhosis, 80 HCV cirrhotic patients free of HCC and 80 healthy controls. Apa1 rs7975232 SNP was detected by PCR- RFLP technique. Routine labor...
Source: British Journal of Biomedical Science - November 6, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Links between SNPs in TLR-2 and TLR-4 and idiopathic recurrent pregnancy loss.
Conclusion: These findings confirm positive associations of TLR-2 rs1898830 with RPL, further supporting a role for TLR signaling in defining pregnancy outcome. PMID: 31679469 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - November 5, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

miR-559 polymorphism rs58450758 is linked to breast cancer.
Authors: Bahreini F, Ramezani S, Shahangian SS, Salehi Z, Mashayekhi F Abstract Background: MicroRNAs (miRNAs) participate in gene regulation and the control of cancer-related mechanisms such as apoptosis, invasion and differentiation. Single nucleotide polymorphisms (SNP) of the miRNA encoding genes may influence the development of cancer. We hypothesised a link between miR-559 SNP rs58450758 and breast cancer. Materials & methods: Bioinformatics analyses were performed to predict the miR-559 target genes and the effect of the rs58450758 SNP on the stem-loop structure. A total of 129 breast cancer cases and 15...
Source: British Journal of Biomedical Science - October 24, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Single Nucleotide Polymorphisms rs12979860 and rs8099917 in IL-28B and Spontaneous Clearance of Hepatitis C Genotype 4.
Conclusions The findings of the present study highlights protective roles for C and T genotypes of rs12979860 and rs8099917 respectively and its association with spontaneous clearance of HCV genotype 4. PMID: 31642723 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - October 24, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Non-coding RNAs - A primer for the laboratory scientist.
The objective of this brief review is to explain the molecular genetic of these molecules, to summarise their potential as mediators of disease, and to highlight their value as diagnostic markers and as tools in disease management. PMID: 31594453 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - October 10, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Relationship between expression levels of circulating hsa-miR-125a-3p and hsa-miR-125b with breast cancer.
Authors: Ramezani S, Talesh Sasani S, Fakor F, Alizadehsefat S Abstract Breast cancer is a major leading cause of cancer death in women in almost all countries,including developing countries. Circulating microRNAs have been detected as potential blood-based biomarkers for the prognosis and diagnosis of various diseases and malignancies, including breast cancer. we analyzed miR-125a-3p and miR-125b expression patterns in breast cancer women in comparison to healthy controls in Guilan province, Iran. Peripheral blood sampling was done from 90 breast cancer patients and the exprssion levels of the miRNAs assessed usin...
Source: British Journal of Biomedical Science - October 3, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of SNPs in PLA2R1 with idiopathic and secondary membranous nephropathy in two Chinese cohorts.
Authors: Tian C, Li L, Qiu P, Qiu Y Abstract Objectives: Specific single-nucleotide polymorphisms (SNPs) in the M-type phospholipase A2 receptor-1 (PLA2R1) are associated with increased risk of idiopathic membranous nephropathy (IMN) in European populations. We hypothesised links between IMN and SMN with these SNPs in two Chinese cohorts. Methods: A cohort of 166 IMN patients and 144 controls from southern China (Group A) and a cohort of 212 IMN patients, 118 SMN patients, and 162 controls from northwestern China (Group B) were recruited. SNPs within PLA2R1 (rs3749119, rs3749117, rs35771982, rs3828323, and rs466430...
Source: British Journal of Biomedical Science - September 20, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Elevated serum alkaline phosphatase in epilepsy: Effect of age and treatment.
The objective of the present study was to investigate the effect of antiepileptic drug therapy on the hematobiochemical parameters in patients with epilepsy and to investigate the association of altered parameters with different demographic and clinical variables. Methods: A cross-sectional study involving 567 patients with epilepsy (drug-naive, N=223; phenytoin, N=85; carbamazepine, N=114; valproate, N=100; and multitherapy, N=45) and 37 healthy controls, aged 5-60 years, from North India was conducted. Hematological and biochemical profiling of all the subjects was performed at a single center. Results: Of all the parame...
Source: British Journal of Biomedical Science - September 7, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

GSTT1, Calpain 10 SNP 19 and indices of glycaemia in type 2 Diabetes.
Authors: Osman H, Osman MY, Alsaidy AA PMID: 31460838 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - August 30, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Clinical diagnostic significance of combinations of 14-3-3 η protein, high-mobility group box-1, anti-cyclic citrullinated peptide antibodies, anti-mutated citrullinated vimentin anti-bodies and rheumatoid factor in rheumatoid arthritis.
Conclusions: Despite individual increases in serum 14-3-3η, HMGB1, anti-CCP, anti-MCV and RF, the combination of anti-CCP and anti-MCV might be of great help for diagnostic in RA, and so should be considered as routine tests for this disease. PMID: 31433746 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - August 23, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Rapidly Progressive Case of Type I Madelung Disease with Bilateral Parotid and Minor Salivary Glands Involvement.
We report a case of an obese 56 year old woman who presented with a sudden unexplained progression of swelling in the neck and upper thorax region associated by severe dyspnea. She was admitted with angioedema, and radiology images identified a bilateral adipose degeneration of the parotid and minor salivary glands. Several medical specialists were consulted regarding differential diagnoses such as Cushing's syndrome, but ultimately diagnosed Type 1 Madelung Disease. The patient will be kept under periodic observation and if any deterioration takes place, surgical intervention will be taken under consideration. Despite no ...
Source: British Journal of Biomedical Science - August 21, 2019 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research