XPG gene polymorphisms and glioma susceptibility: a two-center case-control study.
CONCLUSION: Certain variants of XPG are risk factors for paediatric glioma, and so may be useful in early diagnosis. PMID: 33393424 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 6, 2021 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Increased serum CA125 II, but not CEA, CA19-9, AFP, or CA72-4 in colon cancer compared to rectal cancer.
Authors: Liu T, Li X, Liu D, Liu S, Dong M PMID: 33393429 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 6, 2021 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Interaction between LncRNAs HOTAIR and MALAT1 tagSNPs in Gastric Cancer.
Authors: Abdi E, Latifi-Navid S, Kholghi-Oskooei V, Pourfarzi F, Yazdanbod A PMID: 33332245 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 18, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association Analysis of KISS1 Polymorphisms and Haplotypes with Polycystic Ovary Syndrome.
Authors: Farsimadan M, Moammadzadeh Ghosi F, Takamoli S, Vaziri H Abstract INTRODUCTION: KISS1 play an essential role in human reproductive functions by regulating the hypothalamic-pituitary- gonadal axis. Loss-of-function mutations in this gene have been frequently identified in patients with different reproductive disorders. We hypothesised links between KISS1 polymorphisms and polycystic ovary syndrome (PCOS). MATERIALS AND METHODS: In order to find links between KISS1 polymorphisms rs4889 C>G, rs12998 G>A, and rs35431622 A>G with PCOS, 770 blood samples were obtained from 385 control and 385 PCOS w...
Source: British Journal of Biomedical Science - December 15, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Analytical evaluation and critical appraisal of early commercial SARS-CoV-2 immunoassays for routine use in a diagnostic laboratory.
CONCLUSIONS: The Roche assay and the Euroimmun NC and S1 assays had the best sensitivity overall. Combining the assays detecting NC and S1/S2 antibody increased diagnostic yield. These first-generation assays were not calibrated against reference material and the results were reported qualitatively. A portfolio of next generation SARS-CoV-2 immunoassays will be necessary to investigate herd and vaccine induced immunity. PMID: 33308026 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 15, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Endoscopic management of acute oesophageal variceal bleeding within 12 hours is superior to 12-24 hours post admission.
Authors: Mousa N, Abdel-Razik A, Sheta T, G Deiab A, Habib A, El-Diasty M, Eldesoky A, Taha A, Mousa E, Yassen A, Fathy A, Elgamal A Abstract BACKGROUND AND AIMS: Acute oesophageal variceal haemorrhage (AOVH) is a medical emergency. The American Association for the Study of Liver Diseases (AASLD) recommended endoscopy management as soon as possible and not more than 12 hours after presentation. The United King guidelines recommended endoscopy for unstable patients with severe acute upper gastrointestinal bleeding immediately after resuscitation and within 24 hours of admission. The aim of the study is to evaluate t...
Source: British Journal of Biomedical Science - December 13, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

KISS1R polymorphism rs587777844 (Tyr313His) is linked to female infertility.
Authors: Siahpoosh Z, Farsimadan M, Pazhohan M, Vaziri H, Mahmoudi Gomari M PMID: 33275081 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 5, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

British Journal of Biomedical Science in 2020. What have we learned?
Authors: Brown NW, Orchard G, Rhodes A Abstract Each year the British Journal of Biomedical Science publishes a 'What have we learned' editorial designed to introduce readers within the major disciplines of laboratory medicine to developments outside their immediate area. In addition it is designed to inform a wider readership of the advances in the diagnosis and treatment of disease. To this end, in 2020 the journal published 39 articles covering the disciplines within Biomedical Science in the 4 issues comprising volume 77. These included a review of COVID-19 in this issue, 27 original articles, 6 Biomedical Scie...
Source: British Journal of Biomedical Science - December 1, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Certain haplotype of the 3'-UTR region of the HLA-G gene are linked to breast cancer.
CONCLUSION: Certain variants in the 3-UTR, and their combination as a haplotype, of the HLA-G gene are linked to breast cancer. PMID: 33237834 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - November 27, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Long non-coding RNA EGFR-AS1 in colorectal cancer: A potential factor in tumorigenesis and survival via miRNA-133b sponge and EGFR/STAT3 axis regulation.
Authors: Atef MM, Amer AI, Hafez YM, Elsebaey MA, Saber SA, Abd El-Khalik SR Abstract BACKGROUND: Colorectal cancer is one of the most common cancers worldwide and a major cause of cancer-related death. Thus molecular biomarkers for colorectal cancer have been proposed. The role of long non-coding RNA EGFR-AS1 in colorectal cancer is still unclear. We aimed to evaluate its expression in different stages of colorectal cancer and determine any possible role in regulating the miR‑133b/EGFR/STAT3 signaling pathway. MATERIALS AND METHODS: The relative expression of EGFR-AS1 and miR‑133b were evaluated by quantit...
Source: British Journal of Biomedical Science - November 21, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Long non-coding RNA ANRIL polymorphisms in papillary thyroid cancer and its severity.
Conclusions: Certain haplotypes of ANRIL SNPs are associated with papillary thyroid cancer. ANRIL rs1333048 and rs4977574 variants were associated with larger and smaller tumor sizes, respectively. rs10757274 and rs1333040 variants might lead to lower III-IV cancer stages. These SNPs may be important in the diagnosis of this form of thyroid cancer. PMID: 33186076 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - November 17, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Evaluation of different haematoxylin stain subtypes for the optimal microscopic interpretation of cutaneous malignancy in Mohs frozen section histological procedure.
This study aimed to determine which mordant based haematoxylin (Ehrlich's, Cole's, Mayer's, Gill's I, Gill's II, Gill's III, Weigert's, Harris' or Carazzi's) produced the most optimal morphological clarity of staining for the identification of cellular and tissue morphology of cutaneous basal cell carcinoma (BCC), as part of the Mohs procedure. MATERIAL AND METHODS: In total, 100 anonymised patient cases were selected, sectioned and stained with each haematoxylin subtype. The slides were then independently evaluated microscopically by two assessors. A combined score was generated to determine the sensitivity (defined a...
Source: British Journal of Biomedical Science - October 21, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

HCC-Mark: a simple noninvasive model based on routine parameters for predicting hepatitis C virus related hepatocellular carcinoma.
Authors: Omar M, Farid K, Emran T, El-Taweel F, Tabll A, Omran M Abstract A b s t r a c t. BACKGROUND: Early detection of hepatocellular carcinoma (HCC) is crucial in providing more effective therapies. As routine laboratory variables are readily accessible, this study aimed to develop a simple noninvasive model for predicting hepatocellular cancer. METHODS: Two groups of patients were recruited: an estimation group (n= 300) and a validation group (n= 625). Each comprised 2 categories: hepatocellular cancer and liver cirrhosis. Logistic regression analyses and receiver operating characteristic (ROCs) curves...
Source: British Journal of Biomedical Science - October 6, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Next-generation sequencing-based clinical metagenomics identifies Prevotella pleuritdis in a diabetic adolescent with large parapneumonic effusion and negative growth of pleural fluid culture: a case report.
Authors: Galliguez T, Tsou PY, Cabrera A, Fergie J PMID: 32993476 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - October 2, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Understanding the dynamics of COVID-19; implications for therapeutic intervention, vaccine development and movement control.
Authors: Salvamani S, Tan HZ, Thang WJ, Ter HC, Wa MS, Gunasekaran B, Rhodes A Abstract The COVID-19 disease is caused by the recently identified SARS-CoV-2 virus, thought to have originated in bats (Rhinolophus affinis), the virus being highly infective within the human population and spread by respiratory droplets, contaminated surfaces and close person-to-person contact. The virus is now pandemic and widely disseminated to almost every continent and nation with globally over twenty-seven million infections and over ninety-thousand reported deaths attributed to the COVID-19 disease. SARS-CoV-2 is a single strande...
Source: British Journal of Biomedical Science - September 21, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

OX40 genetic variations in patients with breast cancer: a case-control study.
Authors: Faghih Z, Taherifard E, Daneshmand A, Talei A, Erfani N PMID: 32921275 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - September 16, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Pseudomonas aeruginosa - Candida interplay: Effect on in vitro antibiotic susceptibility of Pseudomonas aeruginosa when grown in the presence Candida culture.
Authors: McIlroy R, Millar BC, Nelson DW, Murphy A, Rao JR, Downey DG, Moore JE PMID: 32887537 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - September 6, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Value of serum collagen triple helix repeat containing-1(CTHRC1) and 14-3-3 η protein compared to anti-CCP antibodies and anti-MCV antibodies in the diagnosis of rheumatoid arthritis.
This study explored the clinical value of collagen triple helix repeat containing-1 (CTHRC1) and 14-3-3η protein, compared to routine markers, in the diagnosis of RA. METHODS: We recruited 103 RA patients, 105 non-RA patients (osteoarthritis, ankylosing spondylitis, systemic lupus erythematosus) and 59 healthy controls. CTHRC1, 14-3-3η, anti-cyclic citrullinated peptide antibody (anti-CCP), anti-mutated citrullinated vimentin antibody (anti-MCV), rheumatoid factor and erythrocyte sedimentation rate (ESR) levels were measured, and their diagnostic value for RA evaluated and compared. RESULTS: All laboratory ...
Source: British Journal of Biomedical Science - August 20, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The effects of human immunodeficiency virus, human papillomavirus, herpes simplex virus-1 and -2, human herpesvirus-6 and -8, cytomegalovirus, and hepatitis B and C virus on female fertility and pregnancy.
We present an overview of the impact of common viral infections on female reproduction, searching Medline, PubMed, Scopus, and Google scholar databases to find the potentially relevant studies of viruses known to have a potential effect. Viruses such as human immunodeficiency virus (HIV), herpes simplex virus (HSV) and human herpesvirus (HHV) increased infertility rates whilst human papillomavirus (HPV), cytomegalovirus (CMV), and hepatitis B and C virus (HBV, HCV) infections mostly led to higher rates of abortion and miscarriage. Moreover, HPV infection is linked to increased tubal infertility, endometriosis, and pelvic i...
Source: British Journal of Biomedical Science - July 31, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Efficacy of laparoscopic gastric bypass vs laparoscopic sleeve gastrectomy in treating obesity combined with type-2 diabetes.
Authors: Yan Y, Wang F, Chen H, Zhao X, Yin D, Hui Y, Ma N, Yang C, Zheng Z, Zhang T, Xu N, Wang G Abstract OBJECTIVE: This prospective study compared the efficacy and safety of laparoscopic gastric bypass and laparoscopic sleeve gastrectomy in treating overweight and obese patients with BMI>28 kg/m2 and type-2 diabetes. METHODS: Patients were randomized into a gastric bypass group (n=77) or a gastrectomy group (n=80). The surgery time, intraoperative blood loss, recovery time, and hospitalization time were collected. BMI, waistline, hipline, C-peptide level, insulin resistance index (HOMA-IR), and their blo...
Source: British Journal of Biomedical Science - July 24, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The relationships between maternal and placental polymorphisms of miR-196a2 and miRNA-499 genes and preeclampsia.
CONCLUSION: The placental but not maternal miRNA-196a2 rs11614913 variant could be a protective factor for preeclampsia predisposition in all models except the recessive model. The maternal/placental rs3746444 CC genotype was in association with higher preeclampsia risk. PMID: 32686997 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 21, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Overexpression of a Novel Long Non-Coding RNA MCM3AP-AS1 in breast cancer tissues compared to adjacent non-tumour tissues.
Authors: Riahi A, Hosseinpour-Feizi M, Rajabi A, Akbarzadeh M, Montazeri V, Safaralizadeh R Abstract BACKGROUND: Altered expression of several long non-coding RNAs (lncRNAs) have been described in numerous malignancies, including breast cancer, and some may have a role in carcinogenesis. We hypothesised differences in the expression of lncRNA MCM3AP-AS1 in breast cancer tissues compared to nearby healthy tissues and potential links with clinical features. METHODS: We tested our hypothesis in 102 pairs of breast cancer tumours and adjacent non-tumour tissues from female patients. After RNA extraction, cDNA synth...
Source: British Journal of Biomedical Science - July 18, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Serum long noncoding RNAs FAS-AS1 & PVT1 are novel biomarkers for systemic lupus erythematous.
Serum long noncoding RNAs FAS-AS1 & PVT1 are novel biomarkers for systemic lupus erythematous. Br J Biomed Sci. 2020 Jul 02;:1-5 Authors: Ali MA, Shaker OG, Khalefa AA, Abdelwahed MY, Ali E, Ezzat EM, Elghobary HA, Awaji AA, Fouad NA, Ayoub SE Abstract BACKGROUND: Systemic Lupus Erythematous (SLE) is a chronic systemic autoimmune disorder whose diagnosis depends on combination of multiple factors. Circulating lncRNAs could serve as diagnostic non-invasive biomarkers for SLE. We hypothesised that serum FAS-AS1 and PVT1 are new biomarkers for SLE that relate to clinical features and laboratory marker...
Source: British Journal of Biomedical Science - July 4, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association between SLCO1B1 polymorphism distribution frequency and blood lipid level in Chinese adults.
Authors: Mo Q, Huang S, Ma J, Zhang J, Su R, Deng Q Abstract BACKGROUND: The variation of serum lipid levels can be part-related to certain genes. One such gene, SLCO1B1, encodes a transporter that may have a role in lipid metabolism. We hypothesised that differences in certain SLCO1B1 genotypes are related to levels of serum lipids. MATERIALS AND METHODS: We recruited genotyped 636 subjects who were genotyped for SLCO1B1 variants *1a, *1b, *5 and *15. Routine liver function tests, renal function tests and routine lipid indices were measured by standard techniques. RESULTS: The most frequent genotypes were ...
Source: British Journal of Biomedical Science - July 1, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of miR-146a rs57095329 with Behcet's disease and its complications.
Authors: Abdelaleem OO, Fouad NA, Shaker OG, Hussein HA, Ahmed FA, Ali DY, Elsayed HS Abstract BACKGROUND: Behçet's disease is a chronic relapsing and remitting autoimmune multisystem inflammatory disease characterised by oral aphthae, genital ulcers, skin lesions, gastrointestinal involvement, arthritis, vascular lesions, and neurological manifestations. We hypothesised a link between rs57095329 of miR-146a and Behçet's disease, with further links with common clinical features. METHODS: We tested our hypothesis in 130 Behçet's disease patients and 131 age and sex-matched healthy controls. ...
Source: British Journal of Biomedical Science - June 24, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Toll-like receptor 7 mRNA is reduced in hepatitis C based liver cirrhosis and hepatocellular carcinoma, out-performs alpha-feto protein levels, and with age and serum aspartate aminotransferase is a new diagnostic index.
Authors: Mohamed AA, Omran D, El-Feky S, Darwish H, Kassas A, Farouk A, Ezzat O, Abdo SM, Zahran FE, El-Demery A, Omran MM Abstract BACKGROUND: Hepatitis B and C viruses are leading causes of liver cirrhosis and hepatocellular carcinoma (HCC). Toll-like receptor 7 (TLR7) has been implicated in the pathogenesis of HCC linked to hepatitis B. We hypothesised a role of leukocyte TLR7 mRNA in hepatitis C related liver cirrhosis and HCC, using alpha fetoprotein (AFP) and liver function tests as comparators. METHODS: We recruited 102 patients with HCV-related HCC, 97 with HCV related liver cirrhosis and 60 healthy con...
Source: British Journal of Biomedical Science - June 24, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Atypical and novel presentations of Coronavirus Disease 2019: a case series of three children.
We report three children whose initial presentation was gastrointestinal, and in whom COVID-19 infection was found, concluding that cases of acute appendicitis, mesenteric adenitis and flank tenderness may mask and infection with this virus, which should therefore be investigated. PMID: 32552415 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 20, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A novel serum metabolome score for breast cancer diagnosis.
Authors: Rashed R, Darwish H, Omran M, Belal A, Zahran F Abstract INTRODUCTION: Early detection of breast cancer is helpful in the diagnosis and treatment, and so in enhancing patient survival and reducing death rates. Because of the low diagnostic sensitivity and specificity of widely used breast cancer biomarkers such as CA15-3, we hypothesised that a panel of new metabolic markers would provide superior sensitivity and specificity for this disease. MATERIAL & METHODS: We recruited 120 women cases with malignant breast cancer, 47 with benign breast disease and 55 females as a healthy control group. Metabo...
Source: British Journal of Biomedical Science - June 19, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Haplotype-based association study of Opioid Receptor Kappa-type 1 (OPRK1) gene polymorphisms with nicotine dependence among male smokers.
Authors: Albonaim A, Sharafshah A, Keshavarz P PMID: 32508243 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 10, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Fine structure of the human retina defined by confocal microscopic immunohistochemistry.
CONCLUSION: The multiple labelling of human retinal cells brings further understand of the biological characteristics and functions of these cells, and provides a theoretical basis for their possible role in diseases. In the growing field of human retina research, our data may provide a point of reference for future studies of the human retina. PMID: 32498649 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 7, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Epidermal growth factor +61A/G (rs4444903) promoter polymorphism and serum levels are linked to idiopathic male infertility.
Authors: Malek MA, Mashayekhi F, Salehi Z PMID: 32448090 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 27, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Circulating Micro RNA-223 and Angiopoietin-Like Protein 8 as Biomarkers of Gestational Diabetes mellitus.
Authors: Abdeltawab A, Zaki MES, Abd El Dayem Y, Mohamed AA, Abbas S Abstract Background: Gestational diabetes mellitus (GDM) is a serious health problem associated with both fetal and maternal complications. New biomarkers that can predict or help in the early diagnosis of GDM are needed to minimize the hazards of hyperglycemia on pregnant women and their offspring. We hypothesised a link between levels of microRNA-223 (miRNA-223) and Angiopoietin-Like Protein 8 (ANGPTL8) and GDM.Materials and Methods: The study included 109 patients with confirmed early diagnosed GDM and 103 healthy control pregnant women in thei...
Source: British Journal of Biomedical Science - May 19, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of hTERT SNP (rs2736100) with implantation failure after in vitro fertilization and embryo transfer (IVF-ET).
Authors: Tajalli S, Mashayekhi F, Salehi Z, Arefi S, Sasani ST PMID: 32401182 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 15, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of Estrogen Receptor Alpha gene SNPs Arg157Ter C > T and Val364Glu with Female Infertility.
Association of Estrogen Receptor Alpha gene SNPs Arg157Ter C>T and Val364Glu with Female Infertility. Br J Biomed Sci. 2020 Apr 21; Authors: Zaer SJ, Farsimadan M, Abiri A, Sharafshah A, Vaziri H PMID: 32316865 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - April 24, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Nicotinic acetylcholine receptors (rs1051730) gene polymorphism and Surfactant protein D level in chronic obstructive pulmonary disease.
Authors: El Gayed EA, Elhelbawy RH, Elhelbawy NG Abstract Chronic obstructive pulmonary disease (COPD) is a complicated disorder that may be genetically determined. It is possible that genetic determinants of nicotine addiction contribute to COPD burden. Surfactant protein D (SP-D) ensures correct ventilatory function . The aim of this work is to study serum level of (SP-D) and nicotinic acetylcholine receptors (nAChRs) (rs1051730) (G/A) gene polymorphism in COPD. We recruited 80 patients with COPD and 80 ages and gender matched healthy individuals. Estimation of Serum SP-D level (by ELISA) and genotyping of (nAChR...
Source: British Journal of Biomedical Science - April 18, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Prognostic value of bone marrow MUC4 expression in acute myeloid leukemia.
CONCLUSION: MUC4 over-expression is an independent predictor of a poor prognosis in AML patients. PMID: 32270747 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - April 11, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Demonstration of microRNA using in situ hybridisation on formalin fixed paraffin wax samples using conventional oligonucleotide probes: A comparison with the use of locked nucleic acid probes.
Conclusion: This study has shown that simplification of ISH protocols by the use of COPs provides equivalent results to the use of LNA methods and it can be used to precisely identify cells in which miRNAs are expressed. PMID: 32223721 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - April 1, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Polymorphisms rs763110 in FASL is linked to hepatitis C virus infection among high-risk populations.
Authors: Huang P, Wang C, Zhuo L, Xia X, Yang S, Zhang J, Fan H, Wu J, Bin Y, Yue M, Zhang Y Abstract Background: The Fas cell surface death receptor (FAS) and Fas ligand (FASL) can participate in the apoptosis of immune cells and target cells infected with a virus through the FAS-FASL signaling pathway. The decoy receptor 3 (DCR3) can competitively inhibit the binding of FAS to FASL. Our aim is to investigate the effect of single nucleotide polymorphisms (SNPs) in FAS, FASL and DCR3 on hepatitis C virus (HCV) infection.Methods: Four SNPs (rs763110 in FASL, rs1324551 and rs2234767 in FAS and rs2257440 in DCR3) were...
Source: British Journal of Biomedical Science - March 28, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Serum LINC00305 expression and its genetic variant rs2850711 are associated with clinical and laboratory features of rheumatoid arthritis.
Authors: Wahba AS, Ibrahim ME, Mesbah NM, Saleh SM, Abo-Elmatty DM, Mehanna ET Abstract Background: Long intergenic non-Protein coding (lnc) RNA 00305 (LINC00305) is a pro-inflammatory atherosclerosis-associated lncRNA. We hypothesised altered LINC00305 variant rs2850711 (A/T) in rheumatoid arthritis (RA) patients and links with clinical and routine laboratory markers.Methods: 100 RA patients and 100 healthy controls were recruited. LINC00305 genotyping and expression were performed using allelic-discrimination PCR and quantitative real-time PCR. LINC00305 diagnostic power was evaluated using area under the receive...
Source: British Journal of Biomedical Science - March 21, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

PADI4 (rs2240340), PDCD1 (rs10204525), and CTLA4 (231775) Gene Polymorphisms and Polyarticular Juvenile Idiopathic Arthritis.
Authors: Ali M, Kaddafy S, Abdelaziz A, Abonar A, Hanafy M, Hussein H, Shabana H, Abd El-Hmid R, Ali M Abstract Background: Certain single nucleotide polymorphisms (SNPs) in genes such as PAD14 (coding for peptidyl arginine deiminase 4), PDCD1 (coding for programmed cell death 1), and CTLA4 (coding for cytotoxic T-lymphocyte-associated protein 4) are linked to rheumatoid arthritis (RA). However, links between SNPs rs2240340, rs10204525 and rs231775 in PAD14, PDCD1 and CTLA4 respectively, and juvenile idiopathic arthritis (JIA), the commonest type of childhood arthritis are unclear. We aimed to determine whether any...
Source: British Journal of Biomedical Science - March 13, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The Effect of CASP3 rs4647610 and rs4647602 Polymorphisms on Tumour Size and Cancer Stage in Papillary Thyroid Carcinoma.
Conclusion: Although CASP3 rs4647610 and rs4647602 SNPs are not associated with PTC, rs4647610 is linked to larger tumour size, and rs4647602 to lower stage of cancer. PMID: 32151194 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - March 12, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Reduced Sperm Concentration in a Patient from a Suspected Post-Operative Infection: a case study.
Authors: Long S, Dawe S, Woodward B Abstract A diagnostic semen analysis should be performed as part of a couple's routine fertility investigations in order to determine sperm quality prior to managing the treatment pathway. The semen analysis report should be considered alongside clinical discussions and a review of both patients' medical history. However, whilst it is part of the standard patient pathway, a regular up-to-date review at each clinical step of a patients' journey is not always performed, which may miss potential clinical changes that could impact the most effective management of the couple. This cas...
Source: British Journal of Biomedical Science - February 25, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A comparison of the HAIN Genotype CM reverse hybridisation assay with the Bruker MicroFlex LT MALDI-tof mass spectrometer for identification of clinically relevant mycobacterial species.
ConclusionsThis comparative study has shown that the use of MALDI-tof for identification of Mycobacteria has resulted in a reduction in turnaround times, a dramatic reduction in the cost of identification and less preparation time in the laboratory. Overall, the results show that MALDI-tof appears to be a suitable replacement for the Genotype CM assay in the routine clinical Mycobacteriology laboratory and the methods described herein can act as a roadmap for other laboratories to implement the technology successfully. PMID: 32072855 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 21, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Upregulation of miR-410 is linked with unfavorable prognosis in patients with colorectal cancer.
Authors: Abedi P, Bayat A, Ghasemzadeh S, Raad M, Pashaiefar H, Ahmadvand M Abstract Background: Although miR-410 is reported to act as a cancer inducer in colorectal cancer, there is limited data on the clinical implications of miR-410 expression levels in patients. We hypothesised a link between miR-410 expression and its potential clinical values in patients with colorectal cancer.Material and methods: 120 colorectal cancer tissue specimens and 120 adjacent non-tumor tissues were obtained. Quantification of miR-410 expression levels was determined by, quantitative RT-PCR. Expression was analysed by clinical feat...
Source: British Journal of Biomedical Science - February 20, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR.
Authors: Sánchez AG, Juaneda I, Eynard H, Basquiera AL, Palazzo E, Calafat P, Palla V, Romagnoli PA, Alvarellos T Abstract Background: CAT25 (T25mononucleotide repeat of the Caspase 2 gene), is a promising DNA marker for detecting microsatellite instability (MSI) in colorectal cancer. CAT25 has the potential to be incorporated into the Bethesda panel, a commonly used panel of DNA microsatellites, or replace it in its entirety. We aimed to develop and validate a high-resolution melting-PCR (HRM-PCR) method for CAT25 instability detection in clinical samples and study its allelic variation in our population.Me...
Source: British Journal of Biomedical Science - February 20, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Relevance of KISS1 gene polymorphisms in susceptibility to Polycystic Ovary Syndrome and its associated Endocrine and Metabolic disturbances.
Conclusion: Women having rs1213704663C>G variation in KISS1 gene are highly susceptible to PCOS and its associated endocrine and metabolic disturbances (LH and estradiol hypersecretion, and increased LH/FSH).Keywords: Polycystic ovary syndrome, gene polymorphisms, Metabolic and Endocrine disturbances, LH, estradiol. PMID: 32031919 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - February 8, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of rs2620381 polymorphism in miR-627 and gastric cancer.
Conclusion: Presence of the C SNP in miR-627 rs2620381 in linked with gastric cancer, and may be important in pathogenesis. PMID: 31983284 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 29, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Malondialdehyde and C-Reactive Protein as prognostic markers of Hepatocellular Carcinoma.
Authors: S E, Naser M, Tarek B, Tarek S, Mahmoud A, K T, Narmin E, A E, A AR Abstract Background . Hepatocellular carcinoma (HCC) is a common cause of cancer-related mortality. The aim of this study is to evaluate the serum Malondialdehyde (MDA) and C-Reactive Protein (CRP) as prognostic markers among patients with HCC and its correlation with the tumor size, Child-Pugh and MELD score.Methods: The study was carried out on 180 patients with HCC and 180 cirrhotic controls. The patients were classified according to the Child-Pugh classification into Child A (n=50), B (n=62) and C (n=68), according to the MELD score in...
Source: British Journal of Biomedical Science - January 25, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Improving the processing time for the detection of carbapenemase-producing enterobacterales using an evolving algorithm.
Authors: Cafferkey J, O'Connor M, Doyle D, Skally M, Fitzpatrick M, Burns K, O'Connell K, Fitzpatrick F, Smyth E, Humphreys H PMID: 31971872 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 25, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Case report: Xanthogranulomatous pyelonephritis masquerading as cystic renal cell carcinoma.
Conclusion: XGP is a diagnostic and therapeutic dilemma. Partial Nephrectomy may be appropriate in management of XGP in select cases. PMID: 31976820 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 25, 2020 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research