[Next-generation Sequencing Analysis of Bacterial Flora in Bovine Prototheca Mastitic Milk].

[Next-generation Sequencing Analysis of Bacterial Flora in Bovine Prototheca Mastitic Milk]. Med Mycol J. 2018;59(3):E41-E46 Authors: Kano R, Kobayashi Y, Nishikawa A, Murata R, Itou T, Ito T, Suzuki K, Kamata H Abstract Prototheca zopfii is an achlorophyllic algae that causes bovine mastitis, resulting in a reduction in milk production and the secretion of thin, watery milk with white flakes. The aim of the present study was to evaluate the bacterial flora in the udder environment in protothecal mastitis. We used metagenomic next-generation sequencing (NGS) analysis to identify 16S rRNA genes from bacterial flora present in milk samples from protothecal mastitic dairy cows.Seven clinical strains of P. zopfii genotype 2 were isolated from 7 milk samples from 7 cases (Holstein cow) of protothecal mastitis; another 9 milk samples were obtained from 9 normal Holstein cows. The samples were collected in 2017 from cows in one dairy located in the Kushiro region in Hokkaido, Japan, which had a history of protothecal mastitis infection.The NGS produced 10,000 to 15,000 sequences in each DNA sample. To facilitate comparison, we grouped the sequencing results according to the culture-based protothecal mastitis diagnosis; sequences derived from the milk samples obtained from healthy cows were grouped separately.Sequences classified as Streptococcus spp., Pseudomonas spp. and Sphingomonas spp., Caulobacter segnis, Macrococcus caseolyticus, Meth...
Source: Medical Mycology Journal - Category: Biology Tags: Med Mycol J Source Type: research