Mesenchymal stem cells and CXC chemokine receptor 4 overexpression improved the therapeutic effect on colitis via mucosa repair.

Mesenchymal stem cells and CXC chemokine receptor 4 overexpression improved the therapeutic effect on colitis via mucosa repair. Exp Ther Med. 2018 Aug;16(2):821-829 Authors: Chen Z, Chen Q, Du H, Xu L, Wan J Abstract The present study intended to observe the homing capability and therapeutic effect of CXC chemokine receptor 4 (CXCR-4) gene overexpressed bone marrow mesenchymal stem cells (BMSCs) on colitis, and to study the possible mechanisms involved. BMSCs were derived from male BALB/c mice and CXCR-4 gene was transfected into BMSCs by the utilization of the lentiviral vector. The expression of CXCR-4 gene was analyzed and the biological characteristics, and vitality of BMSCs and CXCR-4 gene overexpressed BMSCs (CXCR-BMSCs) were detected. The chemotaxis assay was performed to investigate migration in vitro. Colitis was induced by TNBS in female BALB/c mice. BMSCs and CXCR-BMSCs were injected into experimental models intravenously. The homing of cells was confirmed by fluorescence observation and Sry gene detection. Clinical manifestation and histological changes were also evaluated. The expression levels of occludin and vascular endothelial growth factor (VEGF) were detected to measure mucosal repair. Furthermore, CXCR-4 gene was successfully transfected into BMSCs by the utilization of lentiviral vector. Results indicated that overexpression of CXCR-4 gene did not influence the biological characteristics and vitality of BMSCs, b...
Source: Experimental and Therapeutic Medicine - Category: General Medicine Tags: Exp Ther Med Source Type: research