Feeder-free culture for mouse induced pluripotent stem cells by using UV/ozone surface-modified substrates

In this study, changes in the molecular structure of UV/ozone-modified polystyrene were characterized to optimize the surface chemistry for iPSC. Mouse iPSCs (miPSCs) were cultured on the UV/ozone-modified polystyrene substrates without feeder layers. As a result, large polymeric chains of polystyrene were dissociated into small polymeric chains and oxidized to form ester and carboxylic acid functional groups by the UV/ozone treatment. Moreover, it was suggested that optimal valance of these modified molecules enabled the feeder- and matrix coating-free culture of miPSC with maintaining pluripotency.
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research