In vitro assessment of antimicrobial and genotoxic effect of metallosurfactant based nickel hydroxide nanoparticles against Escherichia coli and its genomic DNA

Publication date: 1 October 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 170Author(s): Varsha Dogra, Gurpreet Kaur, Amanpuneet Kaur, Rajeev Kumar, Sandeep KumarAbstractIn the present study, we have synthesized nickel hydroxide nanosuspensions (Ns) using microemulsion technique. This approach is eco-friendly and makes use of Tween 80 (a non-ionic biocompatible surfactant) and newly synthesized metallosurfactants for the formation of uniform nanoparticles in the form of nanosuspensions (Ns). The nickel hydroxide Ns’s were derived from three different metallosurfactants i.e. NiCTAC (Bishexadecyltrimethylammonium nickel tetrachloride), NiDDA (Bisdodecylamine nickel dichloride) and NiHEXA (bishexadecylamine nickel dichloride). Three different nickel-based metallosurfactants were synthesized and characterized using various methods such as CHN, 1HNMR, and FTIR. Fabrication of nanosuspension was confirmed using different characterization methods such as Transmission electron microscopy (TEM), Field Emission Scanning Electron Microscope (FESEM), Energy Dispersive X-ray Spectroscopy (EDX), X-ray Diffraction (XRD), pH and Zeta potential. These particles were further investigated for their genotoxic and cytotoxic effects on gram-negative bacteria, Escherichia coli (E. coli). Effect of nanosuspensions on E. coli was confirmed using colony forming unit count, agar well diffusion, and gram staining method. Through colony forming unit count method, nanosuspensions influence o...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research