Cytosolic tryparedoxin of Leishmania donovani modulates host immune response in visceral leishmaniasis.

In this study, we purified a ∼17 kDa recombinant cytosolic tryparedoxin (cTXN) protein of Leishmania donovani (rLdcTXN) and investigated its immunological responses in peripheral blood monocytes (PBMC) isolated from VL patients. The protein significantly enhanced the promastigotes count after 96 h of culture showing a direct correlation with parasite growth. Furthermore, stimulation of PBMC isolated from VL patients with rLdcTXN resulted in up-regulation of IL-4 and IL-10 production whereas IL-12 and IFN-γ was significantly down-regulated suggesting a pivotal role of cTXN in provoking the immune suppression during VL. Our study demonstrates the importance of cTXN protein which can potentially modulate the outcome of disease through suppressing host protective Th1 response in VL patients.
PMID: 29554571 [PubMed - as supplied by publisher]

Source: Cytokine - March 16, 2018 Category: Molecular Biology Authors: Suman SS, Amit A, Singh KP, Gupta P, Equbal A, Kumari A, Topno RK, Ravidas V, Pandey K, Bimal S, Das P, Ali V Tags: Cytokine Source Type: research