Alterations in Placental Gene Expression of Pregnant Women With Chronic Chagas Disease.

Alterations in Placental Gene Expression of Pregnant Women With Chronic Chagas Disease. Am J Pathol. 2018 Mar 12;: Authors: Juiz NA, Torrejón I, Burgos M, Fernanda Torres AM, Duffy T, Cayo NM, Tabasco A, Salvo M, Longhi SA, Schijman AG Abstract Infection with Trypanosoma cruzi in women at reproductive age is associated with congenital transmission and adverse pregnancy outcome. The placenta is a key barrier to infection. We characterized gene expression profiles of term placental environment from T. cruzi seropositive (SP) and seronegative (SN) mothers performing RNA-seq. Nine pools of placental RNA paired samples were used: three from SN and six from SP tissues. Each pool consisted of female/male newborns and vaginal/caesarean deliveries binomials. No newborn resulted congenitally infected. T. cruzi satellite DNA quantitative PCR in placental tissues and maternal and neonatal blood, and parasite 18S RT-qPCR from placental RNA were negative, except in three SP women's bloodstream. To identify pathways associated with maternal T. cruzi infection, a gene-set association analysis was implemented: SP placental samples showed overexpression of inflammatory response and lymphocytic activation whereas numerous biosynthetic processes were down-regulated. About 42 genes showed a significant fold-change between SP and SN groups. KISS1 and CGB5 were down-regulated whereas KIF12, HLA-G, PRG2, TAC3, FN1, and ATXN3L were up-regulated. Several exp...
Source: The American Journal of Pathology - Category: Pathology Authors: Tags: Am J Pathol Source Type: research