Differential expression profiles of long noncoding RNA and mRNA in colorectal cancer tissues from patients with lung metastasis.

Differential expression profiles of long noncoding RNA and mRNA in colorectal cancer tissues from patients with lung metastasis. Mol Med Rep. 2018 Feb 08;: Authors: Shu R, Xu Y, Tian Y, Zeng Y, Sun L, Gong F, Lei Y, Wang K, Luo H Abstract Lungs are the most common extra‑abdominal site of metastasis of colorectal cancer (CRC), in which long noncoding RNA (lncRNA) may serve a role. In the present study, a high‑throughput microarray assay was performed to detect lncRNA expression and identify novel targets for further study of lung metastasis in CRC. In the CRC tissues from patients with lung metastasis, 7,632 lncRNA (3,574 upregulated and 4,058 downregulated) and 6,185 mRNA (3,394 upregulated and 2,791 downregulated) were detected to be differentially expressed with a fold change ≥2 and P<0.05 compared with the CRC tissues without metastasis. A total of six differentially regulated lncRNA were confirmed by reverse transcription‑quantitative polymerase chain reaction in 20 pairs of CRC samples. Furthermore, gene ontology and pathway analysis were conducted to predict the possible roles of the identified mRNA. The upregulated mRNA were associated with cell division (biological processes), protein kinase B binding (molecular functions) and cellular components. The downregulated mRNA were associated with cell adhesion, platelet‑derived growth factor binding and membrane components. Pathway analysis determined that the upregula...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research