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Construction and recombinant expression of Pseudomonas aeruginosa truncated exotoxin A in Escherichia coli.

Construction and recombinant expression of Pseudomonas aeruginosa truncated exotoxin A in Escherichia coli. Cell Mol Biol (Noisy-le-grand). 2018 Jan 31;64(1):64-69 Authors: Qaiser H, Aslam F, Iftikhar S, Farooq A Abstract Pseudomonas aeruginosa exotoxin A (PE) is a bacterial toxin composed of three domains namely: cell binding, translocation and enzymatic domain. The cytotoxic activity of PE is attributed to the enzymatic domain, which inhibits protein synthesis through ADP-ribosylation of EF-2. PE can be genetically modified to fight cancer. In this regard, a truncated and modified form of PE was produced that could be used for more potent immunotoxins. This modified form termed PE38KDEL was completely devoid of cell binding domain and parts of translocation domain II and Ib which are reported to be inessential for cytotoxicity of the toxin. The resultant expressed protein consisted of the essential translocation domain II and catalytic subunit (domain Ib, III). The deletions in the exotoxin A gene for truncated protein production were made via overlapping PCR extension. The amplicon was cloned in pTZ57r-T vector for DNA works and sub cloned in pET22b expression vector.  It is demonstrated here that PE38KDEL can be expressed in huge quantities in Escherichia coli by using the recombinant vector PE38KDEL/pET under control of T7 promoter and E. coli host strain BL21 (DE3) CodonPlus. The protein expression was optimized at 0.5 mM IPTG concentration for...
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

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This report is describing the case of a patient who developed parallel two different mechanisms of resistance: A T790 M resistance mutation and a transformation into a small cell neuroendocrine cancer. Under therapy with Osimertinib and chemotherapy with carboplatin and etoposide the tumor responsed partially. PMID: 29466812 [PubMed - as supplied by publisher]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
PMID: 29472505 [PubMed - in process]
Source: American Journal of Health-System Pharmacy : AJHP - Category: Drugs & Pharmacology Authors: Tags: Am J Health Syst Pharm Source Type: research
The objective was to test the in vitro activities of ceftaroline and comparator agents against clinical isolates of Gram-negative and Gram-positive bacteria. Isolates were identified with VITEK II. Susceptibility testing was with E test. A total of 1264 isolates were tested. Compared to other cephalosporins, ceftaroline demonstrated excellent in vitro activities (MIC90, ≤0.5 mg/L) against Escherichia coli, Salmonella spp. and Haemophilus influenzae. When matched with the comparator cephalosporins, ceftaroline demonstrated the greatest activity against methicillin-susceptible Staphylococcus aureus (MSSA), with MIC90...
Source: Journal of Chemotherapy - Category: Cancer & Oncology Tags: J Chemother Source Type: research
Authors: Sun H, Hu P, Du J, Wang X Abstract Chemotherapy is widely administered to patients with advanced lung cancer; however, data regarding chemotherapeutic sensitivity are limited. The present study aimed to investigate the predictive value of inflammatory indexes for chemotherapeutic efficacy in advanced lung cancer. Patients with stage III and IV unresectable lung cancer that were treated with first-line chemotherapy between January 2007 and December 2011 were retrospectively identified, and chemotherapeutic response was evaluated following 2 or 3 chemotherapy cycles. Prior to chemotherapy, hematologic data a...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
Authors: He XJ, Bian EB, Ma CC, Wang C, Wang HL, Zhao B Abstract The long non-coding RNA SPRY4-intronic transcript 1 (SPRY4-IT1) has been shown to promote the progression of cancer; however, the role of SPRY4-IT1 in glioma remains unclear. The present study demonstrated that SPRY4-IT1 expression was markedly increased in glioma tissues and cells compared with normal brain tissues, whereas knockdown of SPRY4-IT1 inhibited cell proliferation, migration, and invasion in U251 cells. Spindle and kinetochore associated complex subunit 2 (SKA2) was found to be a target of SPRY4-IT1 and was downregulated by SPRY4-IT1-knock...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
Conclusions: Peroral arsenic has little effect on local airway immune responses to bacteria but compromises respiratory epithelial barrier integrity, increasing systemic translocation of inhaled pathogens and small molecules. https://doi.org/10.1289/EHP1878 Received: 09 March 2017 Revised: 14 August 2017 Accepted: 16 August 2017 Published: 28 September 2017 Address correspondence to M.B. Fessler, National Institute of Environmental Health Sciences, 111 T.W. Alexander Dr., P.O. Box 12233, Maildrop D2-01, Research Triangle Park, NC 27709 USA. Telephone: (919) 541-3701. Email: fesslerm@niehs.nih.gov *Current address: UN...
Source: EHP Research - Category: Environmental Health Authors: Tags: Research Source Type: research
Basic scienceExploring anti-stem cell therapy in colorectal cancer  Storm EE, Durinck S, de Sousa e Melo F, et al. Targeting PTPRK-RSPO3 colon tumours promotes differentiation and loss of stem cell function. Nature 2016;529:97–100. Adenomatous polyposis coli mutations resulting in activation of the WNT pathway are thought to be a founder event in the majority of human colorectal cancers (CRCs), yet targeting the WNT pathway has not yielded any effective therapies. There is a subset of CRCs that express recurrent gene fusions of members of the R-spondin (RSPO, WNT agonists) that exhibit WNT activation through tra...
Source: Gut - Category: Gastroenterology Authors: Tags: JournalScan Source Type: research
ConclusionOur results imply that EGCG may be useful for prevention of intestinal disorders or bacterial infection in animals/humans.This article is protected by copyright. All rights reserved
Source: Nahrung / Food - Category: Nutrition Authors: Tags: Research Article Source Type: research
Conclusions: We did not find consistent associations between presence of subgingival periodontal pathogens and risk of several cancers, but suggestion of associations with early colonizing pathogens for total and lung cancer. These conclusions are restricted by the small numbers of events and of bacteria evaluated. To clarify the present findings, further research is needed utilizing larger cohorts and more comprehensive assessment of presence and quantity of oral bacteria.Citation Format: Xiaodan Mai, Robert J. Genco, Michael J. LaMonte, Kathleen M. Hovey, Jo L. Freudenheim, Christopher A. Andrews, Jean Wactawski-Wende. P...
Source: Cancer Research - Category: Cancer & Oncology Authors: Tags: Epidemiology Source Type: research
Conclusions: CIMP+ CRC are associated with a distinct microbiome including pks containing bacteria (K. pneumoniae and E. coli) and 2HG producing bacteria (Fusobacterium), raising the possibility that the methylation defect could be contributed to by the microbiome.Citation Format: Ang Sun, Matteo Cesaroni, Christian Jobin, Carlos Barrero, Jaroslav Jelinek, Jacqueline D. Sautter, Thomas E. Rams, Salim Merali, Jean-Pierre Issa. Distinct microbiome in colorectal cancers with the CpG Island Methylator Phenotype. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18...
Source: Cancer Research - Category: Cancer & Oncology Authors: Tags: Tumor Biology Source Type: research
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