Construction and recombinant expression of Pseudomonas aeruginosa truncated exotoxin A in Escherichia coli.

Construction and recombinant expression of Pseudomonas aeruginosa truncated exotoxin A in Escherichia coli. Cell Mol Biol (Noisy-le-grand). 2018 Jan 31;64(1):64-69 Authors: Qaiser H, Aslam F, Iftikhar S, Farooq A Abstract Pseudomonas aeruginosa exotoxin A (PE) is a bacterial toxin composed of three domains namely: cell binding, translocation and enzymatic domain. The cytotoxic activity of PE is attributed to the enzymatic domain, which inhibits protein synthesis through ADP-ribosylation of EF-2. PE can be genetically modified to fight cancer. In this regard, a truncated and modified form of PE was produced that could be used for more potent immunotoxins. This modified form termed PE38KDEL was completely devoid of cell binding domain and parts of translocation domain II and Ib which are reported to be inessential for cytotoxicity of the toxin. The resultant expressed protein consisted of the essential translocation domain II and catalytic subunit (domain Ib, III). The deletions in the exotoxin A gene for truncated protein production were made via overlapping PCR extension. The amplicon was cloned in pTZ57r-T vector for DNA works and sub cloned in pET22b expression vector.  It is demonstrated here that PE38KDEL can be expressed in huge quantities in Escherichia coli by using the recombinant vector PE38KDEL/pET under control of T7 promoter and E. coli host strain BL21 (DE3) CodonPlus. The protein expression was optimized at 0.5 mM IPTG concentration for...
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

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Conclusion: The specific inhibition of the Il-4 signaling, through dupimulab that binds the IL-4 α receptor subunit, would be effective in the specific inhibition of the allergic response in patients with allergic dermatitis or asthma.
Source: Current Signal Transduction Therapy - Category: Molecular Biology Source Type: research
In this study, a simple but sensitive electrochemiluminescence (ECL) biosensor has been designed for HAase detection based on the electrostatic interaction of anionic hyaluronic acid (HA) and cationic luminous reagent Ru(bpy)32+. Ru(bpy)32+ is adsorbed on HA through electrostatic interaction to form HA-Ru(bpy)32+ complex. The Ru(bpy)32+ in the HA-Ru(bpy)32+ complex cannot be filtered out because of its large molecular weight. In the presence of HAase, the HA in HA-Ru(bpy)32+ can be cleaved into fragments by enzymatic hydrolysis. As a result, the Ru(bpy)32+ attached on the HA fragments can be separated from the HA-Ru(bpy)32...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
Publication date: 1 November 2018Source: Inorganica Chimica Acta, Volume 483Author(s): Yeşim Salman, Fırat Barış Barlas, Murat Yavuz, Kerem Kaya, Suna Timur, Fatma Çetin TelliAbstractFour novel dinuclear Cu(II) complexes (1a–4a) have been prepared from ONO-tridentate chiral Schiff base derivatives of chloralose (1–4). All the new complexes (1a–4a) have been characterized by Fourier transform infrared spectroscopy, UV–visible spectroscopy and elemental analysis. The complex 2a was further characterized by X-Ray crystallography. And also, the magnetic susceptibility measurement of this comp...
Source: Inorganica Chimica Acta - Category: Chemistry Source Type: research
Reese Witherspoon and Keith Urban are among the stars joining the sixth Stand Up To Cancer telethon on Sept. 7.
Source: ABC News: Health - Category: Consumer Health News Tags: Health Source Type: news
Fordin: A novel type I ribosome inactivating protein from Vernicia fordii modulates multiple signaling cascades leading to anti-invasive and pro-apoptotic effects in cancer cells in vitro. Int J Oncol. 2018 Jul 04;: Authors: Lu W, Mao Y, Chen X, Ni J, Zhang R, Wang Y, Wang J, Wu L Abstract Fordin, which is derived from Vernicia fordii, is a novel type I ribosome inactivating protein (RIP) with RNA N-glycosidase activity. In the present study, fordin was expressed by Escherichia coli and purified using nickel affinity chromatography. Previous studies have demonstrated RIP toxicity in a v...
Source: International Journal of Oncology - Category: Cancer & Oncology Authors: Tags: Int J Oncol Source Type: research
Conclusions: Peroral arsenic has little effect on local airway immune responses to bacteria but compromises respiratory epithelial barrier integrity, increasing systemic translocation of inhaled pathogens and small molecules. https://doi.org/10.1289/EHP1878 Received: 09 March 2017 Revised: 14 August 2017 Accepted: 16 August 2017 Published: 28 September 2017 Address correspondence to M.B. Fessler, National Institute of Environmental Health Sciences, 111 T.W. Alexander Dr., P.O. Box 12233, Maildrop D2-01, Research Triangle Park, NC 27709 USA. Telephone: (919) 541-3701. Email: fesslerm@niehs.nih.gov *Current address: UN...
Source: EHP Research - Category: Environmental Health Authors: Tags: Research Source Type: research
Basic scienceExploring anti-stem cell therapy in colorectal cancer  Storm EE, Durinck S, de Sousa e Melo F, et al. Targeting PTPRK-RSPO3 colon tumours promotes differentiation and loss of stem cell function. Nature 2016;529:97–100. Adenomatous polyposis coli mutations resulting in activation of the WNT pathway are thought to be a founder event in the majority of human colorectal cancers (CRCs), yet targeting the WNT pathway has not yielded any effective therapies. There is a subset of CRCs that express recurrent gene fusions of members of the R-spondin (RSPO, WNT agonists) that exhibit WNT activation through tra...
Source: Gut - Category: Gastroenterology Authors: Tags: JournalScan Source Type: research
ConclusionOur results imply that EGCG may be useful for prevention of intestinal disorders or bacterial infection in animals/humans.This article is protected by copyright. All rights reserved
Source: Nahrung / Food - Category: Nutrition Authors: Tags: Research Article Source Type: research
Conclusions: We did not find consistent associations between presence of subgingival periodontal pathogens and risk of several cancers, but suggestion of associations with early colonizing pathogens for total and lung cancer. These conclusions are restricted by the small numbers of events and of bacteria evaluated. To clarify the present findings, further research is needed utilizing larger cohorts and more comprehensive assessment of presence and quantity of oral bacteria.Citation Format: Xiaodan Mai, Robert J. Genco, Michael J. LaMonte, Kathleen M. Hovey, Jo L. Freudenheim, Christopher A. Andrews, Jean Wactawski-Wende. P...
Source: Cancer Research - Category: Cancer & Oncology Authors: Tags: Epidemiology Source Type: research
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Source: Cancer Research - Category: Cancer & Oncology Authors: Tags: Tumor Biology Source Type: research
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