Antimicrobial Activity of Ibuprofen Against Cystic Fibrosis Associated Gram-Negative Pathogens.

Antimicrobial Activity of Ibuprofen Against Cystic Fibrosis Associated Gram-Negative Pathogens. Antimicrob Agents Chemother. 2018 Jan 08;: Authors: Shah PN, Marshall-Batty KR, Smolen JA, Tagaev JA, Chen Q, Rodesney CA, Le HH, Gordon VD, Greenberg DE, Cannon CL Abstract Clinical trials have demonstrated the benefits of ibuprofen therapy in cystic fibrosis (CF) patients, an effect that is currently attributed to ibuprofen's anti-inflammatory properties. Yet, a few previous reports demonstrate an antimicrobial activity of ibuprofen as well, although none investigate its direct effects on the pathogens found in the CF lung, which is the focus of this work. Determination of ibuprofen's in vitro antimicrobial activity against Pseudomonas aeruginosa and Burkholderia spp. strains through measurements of endpoint colony-forming units (CFU) and growth kinetics showed that ibuprofen reduces the growth rate and bacterial burden of tested strains in a dose-dependent fashion. In an in vitro Pseudomonas biofilm model, a reduction in the rate of biomass accumulation over 8-h of growth with ibuprofen treatment was observed. Next, an acute Pseudomonas pneumonia model was used to test this antimicrobial activity after oral delivery of ibuprofen. Following intranasal inoculation, ibuprofen-treated mice exhibited lower CFU counts and improved survival compared with control animals. Preliminary biodistribution studies performed after aerosolization of ibu...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research