EVI and MDS/EVI are required for adult intestinal stem cell formation during postembryonic vertebrate development Research

The gene ectopic viral integration site 1 (EVI) and its variant myelodysplastic syndrome 1 (MDS)/EVI encode zinc-finger proteins that have been recognized as important oncogenes in various types of cancer. In contrast to the established role of EVI and MDS/EVI in cancer development, their potential function during vertebrate postembryonic development, especially in organ-specific adult stem cells, is unclear. Amphibian metamorphosis is strikingly similar to postembryonic development around birth in mammals, with both processes taking place when plasma thyroid hormone (T3) levels are high. Using the T3-dependent metamorphosis in Xenopus tropicalis as a model, we show here that high levels of EVI and MDS/EVI are expressed in the intestine at the climax of metamorphosis and are induced by T3. By using the transcription activator–like effector nuclease gene editing technology, we have knocked out both EVI and MDS/EVI and have shown that EVI and MDS/EVI are not essential for embryogenesis and premetamorphosis in X. tropicalis. On the other hand, knocking out EVI and MDS/EVI causes severe retardation in the growth and development of the tadpoles during metamorphosis and leads to tadpole lethality at the climax of metamorphosis. Furthermore, the homozygous-knockout animals have reduced adult intestinal epithelial stem cell proliferation at the end of metamorphosis (for the few that survive through metamorphosis) or during T3-induced metamorphosis. These findings reveal a novel...
Source: FASEB Journal - Category: Biology Authors: Tags: Research Source Type: research

Related Links:

Publication date: Available online 18 October 2018Source: Journal of Molecular LiquidsAuthor(s): H. Abramczyk, A. Imiela, A. ƚliwiƄskaAbstractLipids play an important role in many cellular processes. Raman-driven microscopy is one of the most powerful technique for analyzing the properties of lipids in cellular compartments offering lipid compositional mapping and more precise, faster, simpler, and nondestructive measurements. Recent advances in Raman imaging (RI) have greatly progressed lipidomic analysis, such as lipid droplet content, lipid phenotype, fatty acid composition, spatial distribution and drug treatment res...
Source: Journal of Molecular Liquids - Category: Molecular Biology Source Type: research
Publication date: Available online 18 October 2018Source: Journal of Molecular LiquidsAuthor(s): Mostafa Zamani, Ehsan Naderi, Mozhgan Aghajanzadeh, Mahmoud Naseri, Ali Sharafi, Hossein DanafarAbstractThe present paper aimed to synthesize, using thermal-treatment method, a variety of Co1-XZnxFe2O4-based nanocarriers (NCs) as Dual-controlled and targeted drug delivery systems (DDS) and provide a new structure as NCs suitable for the loading and pH-responsive characteristics of the chemotherapeutic curcumin (CUR). To study the structure, surface morphology, surface charge and magnetic properties of NCs, X-ray diffraction (XR...
Source: Journal of Molecular Liquids - Category: Molecular Biology Source Type: research
Publication date: Available online 18 October 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Paola D'Andrea, Marina Sciancalepore, Katerina Veltruska, Paola Lorenzon, Antonella BandieraAbstractThe biochemical properties of muscle extracellular matrix are essential for stem cell adhesion, motility, proliferation and myogenic development. Recombinant elastin-like polypeptides are synthetic polypeptides that, besides maintaining some properties of the native protein, can be tailored by fusing bioactive sequences to their C-terminal. Our laboratory synthesized several Human Elastin-Like Pol...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - Category: Molecular Biology Source Type: research
This study examined the role of H2S in drug resistance in liver cancer cells.Materials and methodsHuman primary hepatocellular carcinoma cell line (HepG2) and doxorubicin (Dox)-resistant cells were used in this study. Cell survival was analyzed by MTT, Annexin V-FITC/propidium iodide staining and clonogenic assay. Western blotting was used for analysis of protein expression, and immunoprecipitation was used to determine interactions of LXR/RXR.Key findingsThe expression of H2S-generating enzyme cystathionine gamma-lyase (CSE) was inhibited by doxorubicin treatment in HepG2 cells, and H2S sensitized Dox-inhibited cell survi...
Source: Life Sciences - Category: Biology Source Type: research
This study was based on the fact that, overall mass, charge and size of a protein changes after interaction with charged ligands such as metal ions, which affects the electrophoretic movement of the analyte protein under the influence of electroosmotic flow. This alteration in electrophoretic mobility of the analyte is proportional to the strength of interaction between the two species and is utilized to calculate the binding results. The mobility ratio (R) of an EOF marker (injected in same run) and the analyte enzyme was determined and the binding result was expressed as the normalized difference of the mobility ratios o...
Source: Microchemical Journal - Category: Chemistry Source Type: research
Publication date: Available online 18 October 2018Source: Genes &DiseasesAuthor(s): Xingxing Zang
Source: Genes and Diseases - Category: Genetics & Stem Cells Source Type: research
Switching to a better, lower fat diet can reduce the risk of death by 20 percent for colorectal cancer patients, a new American Cancer Society study found. Western diets high in fat raise death risks.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
An Oregon teenager’s innovation could change the way doctors treat pancreatic cancer, a deadly form of the disease that has just a 7% five-year survival rate. Rishab Jain, a 13-year-old from Stoller Middle School in Portland, on Tuesday won the Discovery Education 3M Young Scientist Challenge with an algorithm that uses machine learning to help doctors zero in on the pancreas during cancer treatment. Doing so can be difficult, since the pancreas is often obscured by other organs, and since breathing and other bodily processes can cause it to move around the abdominal area. As a result, doctors sometimes need to deplo...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized Cancer healthytime onetime Source Type: news
Publication date: Available online 18 October 2018Source: Progress in Retinal and Eye ResearchAuthor(s): Gorka Orive, Edorta Santos-Vizcaino, Jose Luis Pedraz, Rosa Maria Hernandez, Julia E. Vela Ramirez, Alireza Dolatshahi-Pirouz, Ali Khademhosseini, Nicholas A. Peppas, Dwaine F. EmerichAbstractMillions of people worldwide suffer from debilitating, progressive, and often permanent loss of vision without any viable treatment options. The complex physiological barriers of the eye contribute to the difficulty in developing novel therapies by limiting our ability to deliver therapeutics in a sustained and controlled manner; e...
Source: Progress in Retinal and Eye Research - Category: Opthalmology Source Type: research
One of the more interesting aspects of the various epigenetic clocks that have been developed in recent years is that it is still largely unknown as to what exactly it is that they are assessing in our aging biochemistry. These clocks are weighted measures of epigenetic markers, such as DNA methylation, over a comparatively small number of genes. The resulting number certainly reflects chronological age, with the best clocks having a margin or error of a few years when assessed over a group of people. There is also sound evidence for it to reflect biological age, the burden of damage and dysfunction, which varies between i...
Source: Fight Aging! - Category: Research Authors: Tags: Medicine, Biotech, Research Source Type: blogs
More News: Cancer | Cancer & Oncology | Epithelial Cancer | Genetics | Hormones | Myelodysplastic Syndrome | Stem Cell Therapy | Stem Cells | Thyroid | Thyroid Cancer | Zinc