TNF α gene polymorphism in steroid resistant nephrotic syndrome

This study was conducted on 250 subjects, 100 healthy children, 50 steroid sensitive nephrotic syndrome (SSNS), and 100 steroid resistant nephrotic syndrome patients (SRNS). Serum concentration of TNFα was measured by enzyme-linked immunosorbent assay (ELISA) technique and TNFα-G308A gene polymorphism was estimated by polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP). Serum concentrations of TNFα significantly increased in SRNS cases when compared to SSNS subgroups. TNF G A, AA, GA + AA genotypes and A allele showed significant association with risk of NS development within healthy control subjects. TNFα-G308A genotypes and alleles did not differ significantly between SSNS and SRNS groups.
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research