Ultrasound ‑targeted microbubbles combined with a peptide nucleic acid binding nuclear localization signal mediate transfection of exogenous genes by improving cytoplasmic and nuclear import.

In conclusion, UTMD combined with AT‑MCB and a PNA‑binding NLS plasmid significantly improved transfection efficiency by increasing cytoplasmic and nuclear DNA import. This method is a promising strategy for the noninvasive and effective delivery of target genes or drugs for the treatment of cardiovascular diseases. PMID: 28990051 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research

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CONCLUSIONS: Taken together, we provide an effective gene therapy approach to AAA in mice by enhancing antiinflammatory and dampening proinflammatory pathways through minicircle-induced augmentation of systemic IL-10 expression. PMID: 29880489 [PubMed - as supplied by publisher]
Source: Arteriosclerosis, Thrombosis and Vascular Biology - Category: Cardiology Authors: Tags: Arterioscler Thromb Vasc Biol Source Type: research
Contrast developer Bracco Imaging is collaborating with longtime partner Shanghai...Read more on AuntMinnie.comRelated Reading: Bracco hits Jubilant with patent complaint on rubidium PET Bracco lands new FDA approval for MultiHance Bracco targets gene therapy for ultrasound contrast Bracco declines comment on Norris gadolinium lawsuit Bracco Imaging acquires SurgVision
Source: AuntMinnie.com Headlines - Category: Radiology Source Type: news
Abstract To improve the non-invasive therapeutic efficacy for ER positive breast cancer (ER+ BC), we fabricated a multifunctional FOXA1 loaded porphyrin microbubble to combine photodynamic therapy (PDT) and gene therapy of FOXA1 knockdown (KD) with ultrasound targeted microbubble destruction (UTMD) technology under the guidance of contrast enhanced ultrasound (CEUS). Cationic porphyrin microbubbles (CpMBs) were firstly fabricated from a porphyrin grafted lipid with two cationic amino groups (PGL-NH2) and fluorocarbon inert gas of C3F8. Porphyrin group in the CpMBs monolayer could be used as a photosensitizer ...
Source: Biomaterials - Category: Materials Science Authors: Tags: Biomaterials Source Type: research
CONCLUSIONS This study demonstrates that transitory BBB disruption using MRgFUS can be a safe and efficient method for site-specific delivery of viral vectors to the brain, raising the potential for noninvasive focal human gene therapy for neurological disorders. PMID: 29701544 [PubMed - as supplied by publisher]
Source: Journal of Neurosurgery - Category: Neurosurgery Authors: Tags: J Neurosurg Source Type: research
Contrast developer Bracco Diagnostics has filed a complaint against radiopharmaceutical...Read more on AuntMinnie.comRelated Reading: Bracco lands new FDA approval for MultiHance Bracco targets gene therapy for ultrasound contrast Bracco declines comment on Norris gadolinium lawsuit PBS report targets price hike for V/Q radiotracers Jubilant Pharma taps new DraxImage president Jubilant DraxImage, Cyclopharm ink deal
Source: AuntMinnie.com Headlines - Category: Radiology Source Type: news
Publication date: Available online 4 April 2018 Source:Clinics and Research in Hepatology and Gastroenterology Author(s): Aurélia Poujois, France Woimant Wilson's disease (WD) is characterised by a deleterious accumulation of copper in the liver and brain. It is one of those rare genetic disorders that benefits from effective and lifelong treatments that have dramatically transformed the prognosis of the disease. In Europe, its clinical prevalence is estimated at between 1.2 and 2/100,000 but the genetic prevalence is higher, at around 1/7000. Incomplete penetrance of the gene or the presence of modifier genes may ...
Source: Clinics and Research in Hepatology and Gastroenterology - Category: Gastroenterology Source Type: research
Authors: Xiong F, Zhang F, Jin Y, Weng Q, Song J, Zhou G, Shin D, Zheng C, Yang X Abstract Purpose: To validate the feasibility of using interventional radiofrequency hyperthermia(RFH) to enhance herpes simplex virus-thymidine kinase (HSV-TK)/ganciclovir (GCV) gene therapy of rat orthotopic hepatic cancer. Material and Methods: Rat hepatocellular carcinoma cells (MCA-RH-7777) were transduced with lentivirus/luciferase gene for optical imaging. In-vitro experiments with the luciferase cells and in-vivo experiments on rats with orthotopic hepatic tumors were divided into four treatment groups: (i) HSV-TK/GCV-medi...
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research
Researchers at Washington University in St. Louis have developed a new technique to deliver genetic material into cells. Called Acoustic Shear Poration (ASP), the method combines ultrasound waves and focused mechanical force to create pores in the ce...
Source: Medgadget - Category: Medical Devices Authors: Tags: Genetics Medicine Oncology Source Type: blogs
To validate the feasibility of using imaging-guided RF hyperthermia (RFH) to enhance intratumorl HSV-TK/Ganciclovir (GCV) suicide gene therapy of ovarian cancers, monitored by optical and ultrasound imaging.
Source: Journal of Vascular and Interventional Radiology : JVIR - Category: Radiology Authors: Tags: Scientific e-Posters Source Type: research
An Italian diagnostic imaging company is teaming with a Chicago startup to study whether microbubbles can deliver personalized gene therapy to treat high LDL cholesterol. Microbubble technology, combined with contrast-enhanced ultrasound, has been used diagnostically for years and more recently has inspired research for treatment of disease. Milan-based Bracco Imaging, S.p.A. is working with Steven Feinstein, a professor of medicine at Rush University Medical Center and president of SonoGene, LLC to pursue the treatment possibilities further. A CEUS researcher, Feinstein is also co-president of the  International Cont...
Source: MDDI - Category: Medical Devices Authors: Tags: R & D Source Type: news
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