Control of cancer stem cell like population by intracellular target identification followed by the treatment with peptide-siRNA complex.

Control of cancer stem cell like population by intracellular target identification followed by the treatment with peptide-siRNA complex. Biochem Biophys Res Commun. 2017 Sep 23;491(3):827-833 Authors: Suh JS, Lee HJ, Nam H, Jo BS, Lee DW, Kim JH, Lee JY, Chung CP, Lee G, Park YJ Abstract Cancer stem cells (CSCs) are a subpopulation of cancer cells and have been known to create cancer reoccurrence during cancer therapy due to their stem cell-like characteristics. However, exact target to control the CSC has not been fully established. Here, we enriched CD44(High) population of MDA-MB-231 cells by CD44 antibody as a CSC marker. By Phospho Antibody Array, CD44(High) population of MDA-MB-231 cells reveals Feline sarcoma-related tyrosine kinase (FER) protein was highly activated. When FER siRNA and low molecular weight protamine (LMWP) as cell penetrating peptides are applied to this population, cancer migration and colony forming ability are inhibited. Moreover, silencing FER using FER siRNA and LMWP conjugates enhances anti-metastasis related factors including E-cadherin, p75 and p63. Taken together, FER is a new marker for targeting breast CSCs and peptide-mediated siRNA method could be an effective and safe way of delivery and be a new therapeutic strategy for targeting breast cancer. PMID: 28554844 [PubMed - indexed for MEDLINE]
Source: Biochemical and Biophysical Research communications - Category: Biochemistry Authors: Tags: Biochem Biophys Res Commun Source Type: research