Inhibition of nuclear factor-kappaB, cyclooxygenase-2, and metalloproteinase-9 expression by flavanols from evening primrose (Oenothera paradoxa) in human colon cancer SW-480 cells

Publication date: October 2017 Source:Journal of Functional Foods, Volume 37 Author(s): Katarzyna Owczarek, Elżbieta Hrabec, Jakub Fichna, Dorota Sosnowska, Maria Koziołkiewicz, Jacek Szymański, Urszula Lewandowska The present study examines the chemical composition of an evening primrose flavanol preparation (EPFP) and its chemopreventive activity in colon cancer SW-480 cells. EPFP was found to inhibit SW-480 cell viability by 67% vs. controls at 150µM catechin equivalents (CE) following 72h incubation (IC50 =65µM CE). EPFP decreased both NF-κB protein expression (33% vs. control at 100µM CE) and phosphorylation at serine 529 of the p65 subunit (49% vs. control at 100µM CE) in SW-480 cells. Moreover, 100µM CE EPFP suppressed COX-2 mRNA expression to 23% of control values and protein expression to 78%. In addition, EPFP reduced MMP-9 protein expression to 24% vs. controls at 100µM CE. These results provide the first evidence that EPFP reduces NF-κB, COX-2, and MMP-9 expression, which may suggest it has cytotoxic, anti-inflammatory, and anti-metastatic activities towards colon cancer cells. Graphical abstract
Source: Journal of Functional Foods - Category: Nutrition Source Type: research