Proline Dehydrogenase-entrapped mesoporous magnetic silica nanomaterial for electrochemical biosensing of L-proline in biological fluids

Publication date: Available online 4 June 2017 Source:Enzyme and Microbial Technology Author(s): Mohammad Hasanzadeh, Soodabeh Hassanpour, Arezoo Saadati Nahr, Nasrin Shadjou, Ahad Mokhtarzadeh, Jalal Mohammadi In this work, physical adsorption was used for immobilization of proline dehydrogenase onto a magnetic mesoporous silica nanomaterial. The immobilization and electrocatalytical activity of proline dehydrogenase entrapped in a magnetic mesoporous silica nanomaterial was studied using cyclic voltammetry, differential pulse voltammetry, and square wave voltammetry. The magnetic mesoporous silica networks having a high surface area (362m2 g−1) exhibited excellent properties for entrapment of proline dehydrogenase. The applied approach led to better resistance to temperature and pH inactivation in comparison to the free enzyme. The electrocatalytic current response of proline dehydrogenase entrapped in a magnetic mesoporous silica nanomaterial towards oxidation of L-proline was maintained in the analytical solution temperature up to 70°C. The entrapped proline dehydrogenase was casted onto a polycysteine-modified glassy carbon electrode. The electrode was evaluated as an electrochemical biosensor for electrooxidation and determination of L-proline in phosphate buffer solution. A cyclic voltammetry study indicated that the oxidation process of proline is irreversible and is diffusion controlled. The electrochemical behavior was further exploited as a sensitive det...
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research