Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts using octyl-agarose beads with very high volumetric activity: Avoiding enzyme release during multilayer production
Publication date: Available online 17 February 2020Source: Enzyme and Microbial TechnologyAuthor(s): Sara Arana-Peña, Nathalia S. Rios, Carmen Mendez-Sanchez, Yuliya Lokha, Luciana R.B. Gonçalves, Roberto Fernández-LafuenteAbstractA strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU). The enzymes were initially immobilized via interfacial activation on octyl-agarose beads, tre...
Source: Enzyme and Microbial Technology - February 17, 2020 Category: Biotechnology Source Type: research

Phenotypic characterization and comparative genome analysis of two strains of thermophilic, anaerobic, cellulolytic-xylanolytic bacterium Herbivorax saccincola
Publication date: May 2020Source: Enzyme and Microbial Technology, Volume 136Author(s): Shimpei Aikawa, Phakhinee Thianheng, Sirilak Baramee, Umbhorn Ungkulpasvich, Chakrit Tachaapaikoon, Rattiya Waeonukul, Patthra Pason, Khanok Ratanakhanokchai, Akihiko KosugiAbstractThe genome sequences of thermophilic, anaerobic, and cellulolytic-xylanolytic bacterium Herbivorax saccincola strains A7 and GGR1 have recently been determined. Although both strains belong to the same species, A7 is alkaliphilic, non-endospore-forming, and ammonium-assimilating, whereas GGR1 is neutrophilic, endospore-forming, and weak-ammonium-assimilating....
Source: Enzyme and Microbial Technology - February 14, 2020 Category: Biotechnology Source Type: research

Biochemical characterization of a novel thermostable DFA I-forming inulin fructotransferases from Streptomyces peucetius subsp. caesius ATCC 27952
In this study, a novel DFA I-forming IFTase was identified and characterized from Streptomyces peucetius subsp. caesius ATCC 27952 (SpIFTase). The specific activity of SpIFTase was determined to be 420.84 ± 6.21 U mg-1 at pH 6.5 and 45 °C. The enzyme exhibited a prominent thermostability with a half-life of 70 min at 70 °C and a structural melting temperature (Tm) of 75.48 °C. The values of Km and kcat/Km of SpIFTase against inulin substrate were 3.08 mM and 199.09 mM-1 s-1, respectively. Furthermore, the catalytic residues were investigated by site-direct...
Source: Enzyme and Microbial Technology - February 10, 2020 Category: Biotechnology Source Type: research

Regulation of Aureofuscin Production by the PAS-LuxR Family Regulator AurJ3M
Publication date: Available online 8 February 2020Source: Enzyme and Microbial TechnologyAuthor(s): Jing Yang, Dexin Xu, Wenchen Yu, Ruobing Hao, Jie WeiAbstractaurJ3M(GenBank:EU697915.1), a 579 bp gene, whose deduced product (192 amino acid) was found to have amino acid sequence homology with some bacterial regulatory proteins. Computer-assisted analysis showed that AurJ3M is PAS-LuxR family regulatory protein, it combines a PAS domain with a helix–turn–helix (HTH) motif of the LuxR type. Gene deletion of aurJ3M from theS.aureofuscus SYAU0709 through gene replacement with the apramycin resistance gene aac (3) ...
Source: Enzyme and Microbial Technology - February 10, 2020 Category: Biotechnology Source Type: research

Engineering and optimization of phosphate-responsive phytase expression in Pichia pastoris yeast for phytate hydrolysis
Publication date: Available online 7 February 2020Source: Enzyme and Microbial TechnologyAuthor(s): Zhenming Xie, Wing-Ping Fong, Paul Wai-Kei TsangAbstractPhytate is the major storage form of phosphorus in plants. It is present in cereals and raw materials of vegetable origin used in animal and human diets. However, non-ruminant animals have little phytase activity in their guts and, therefore, cannot digest phytate. As a result, almost all dietary phytate is discharged into the environment, causing phosphorus pollution. Phytate is also considered as an “antinutrient” for its ability to form insoluble and stab...
Source: Enzyme and Microbial Technology - February 8, 2020 Category: Biotechnology Source Type: research

Engineered Bacillus subtilis harbouring gene of D-tagatose 3-epimerase for the bioconversion of D-fructose into D-psicose through fermentation
Publication date: Available online 4 February 2020Source: Enzyme and Microbial TechnologyAuthor(s): Jun Zhang, Chao Xu, Xiaoyan Chen, Xi Ruan, Yu Zhang, Huijuan Xu, Ying Guo, Jingliang Xu, Pengmei Lv, Zhongming WangAbstractD-psicose, as important rare sugar and epimer of D-fructose on the C-3 position, displays unique health benefits and physiological functions in various fields. The production of D-psicose is currently obtained via enzymatic bioconversion, mostly with of D-tagatose 3-epimerase. In the present study, the isomerization of D-fructose into D-psicose was achieved by constructing engineered food-grade Bacillus ...
Source: Enzyme and Microbial Technology - February 5, 2020 Category: Biotechnology Source Type: research

Phenotypic characterization of thermophilic, anaerobic, cellulolytic-xylanolytic bacterium Herbivorax saccincola by comparative genome analysis
Publication date: Available online 1 February 2020Source: Enzyme and Microbial TechnologyAuthor(s): Shimpei Aikawa, Phakhinee Thianheng, Sirilak Baramee, Umbhorn Ungkulpasvich, Chakrit Tachaapaikoon, Rattiya Waeonukul, Patthra Pason, Khanok Ratanakhanokchai, Akihiko KosugiAbstractThe genome sequences of thermophilic, anaerobic, and cellulolytic-xylanolytic bacterium Herbivorax saccincola strains A7 and GGR1 have recently been determined. Although both strains belong to the same species, A7 is alkaliphilic, non-endospore-forming, and ammonium-assimilating, whereas GGR1 is neutrophilic, endospore-forming, and non-ammo...
Source: Enzyme and Microbial Technology - February 2, 2020 Category: Biotechnology Source Type: research

Direct and selective enzymatic synthesis of trehalose unsaturated fatty acid diesters and evaluation of foaming and emulsifying properties
Publication date: Available online 1 February 2020Source: Enzyme and Microbial TechnologyAuthor(s): Suping Ji, Chengsheng Jia, Dandan Cao, Shusheng Li, Xiaoming ZhangAbstractTrehalose diesters are Gemini-type surfactants that might have better surface activity than conventional surfactants. A one-step method for the preparation of trehalose unsaturated fatty acid diesters has been successfully developed. The yield of trehalose diester of different unsaturated fatty acids was between 78% and 88% under optimal conditions: 25 mmol/L trehalose, 100 mmol/L unsaturated fatty acid, 60 g/L 3 Å mol...
Source: Enzyme and Microbial Technology - February 2, 2020 Category: Biotechnology Source Type: research

Engineering of L-glutamate oxidase as the whole-cell biocatalyst for the improvement of α-ketoglutarate production
In this study, error-prone PCR mutagenesis of Streptomyces mobaraensis LGOX followed by high-throughput screening was performed to yield four single point mutants with improved enzymatic activity, termed F94L, S280T, I282M and H533R. Moreover, site-saturation mutagenesis at these four residues was employed, yielding two additionally improved mutants, termed I282L and H533L. Subsequently, we employed combinatorial mutagenesis of two, three and four point mutants, and the best mutant S280TH533L showed 90% higher enzymatic activity than the wild-type control. The data also showed that the presence of these point mutations gre...
Source: Enzyme and Microbial Technology - February 1, 2020 Category: Biotechnology Source Type: research

Preference for particular lanthanide species and thermal stability of XoxFs in Methylorubrum extorquens strain AM1
In this study, we found that (i) strain AM1 cells preferentially utilize La3+ rather than Nd3+ for growth, (ii) XoxF purified from cells grown with a La3+ and Nd3+ mixture contained a larger proportion of La3+, and (iii) La3+-metallated XoxF has higher activity and thermal stability than Nd3+-metallated XoxF, although (iv) both enzymes showed unchanged surface charges. Thermal shift assay in particular revealed that metallation affects the temperature for subunit denaturation but not for subunit dissociation. We concluded that, although La3+ and Nd3+ have similar distributions in nature, XoxF could chose La3+ preferentiall...
Source: Enzyme and Microbial Technology - February 1, 2020 Category: Biotechnology Source Type: research

The membrane-bound sorbosone dehydrogenase of Gluconacetobacter liquefaciens is a pyrroloquinoline quinone-dependent enzyme
Publication date: Available online 28 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): Toshiharu Yakushi, Ryota Takahashi, Minenosuke Matsutani, Naoya Kataoka, Roque A. Hours, Yoshitaka Ano, Osao Adachi, Kazunobu MatsushitaAbstractMembrane-bound sorbosone dehydrogenase (SNDH) of Gluconacetobacter liquefaciens oxidizes L-sorbosone to 2-keto-L-gulonic acid (2KGLA), a key intermediate in vitamin C production. We constructed recombinant Escherichia coli and Gluconobacter strains harboring plasmids carrying the sndh gene from Ga. liquefaciens strain RCTMR10 to identify the prosthetic group of SNDH. The membranes of...
Source: Enzyme and Microbial Technology - January 29, 2020 Category: Biotechnology Source Type: research

CRISPR/Cas9 facilitates rapid generation of constitutive forms of transcription factors in Aspergillus niger through specific on-site genomic mutations resulting in increased saccharification of plant biomass
In this study, we show that CRISPR/Cas9 can also be used to efficiently change functional properties of the proteins encoded by the target gene by on-site genomic mutations in A. niger. The obtained strains with constitutively active XlnR and GaaR versions resulted in increased production of plant biomass degrading enzymes and improved release of D-xylose and L-arabinose from wheat bran, and D-galacturonic acid from sugar beet pulp. (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - January 26, 2020 Category: Biotechnology Source Type: research

Semi-rational approach to Expand the Acyl-CoA Chain Length Tolerance of Sphingomonas paucimobilis Serine Palmitoyltransferase
Publication date: Available online 21 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): Hyunjun Choe, Minsun Cha, Jon D. StewartAbstractSerine palmitoyltransferase (SPTase), the first enzyme of the sphingolipid biosynthesis pathway, produces 3-ketodihydrosphingosine by a Claisen-like condensation / decarboxylation reaction of L-Ser and palmitoyl-CoA (n-C16-CoA). Previous structural analysis of Sphingomonas paucimobilis SPTase (SpSPTase) revealed a dynamic active site loop (RPPATP; amino acids 378 - 383) in which R378 (underlined) forms a salt bridge with the carboxylic acid group of the PLP : L-Ser external ald...
Source: Enzyme and Microbial Technology - January 22, 2020 Category: Biotechnology Source Type: research

Enzymatic epoxidation of cyclohexene by peroxidase immobilization on a textile and an adapted reactor design
Publication date: Available online 20 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): D.S. Wunschik, Kim N. Ingenbosch, P. Süss, U. Liebelt, S. Quint, M. Dyllick-Brenzinger, R. Zuhse, U. Menyes, K. Hoffmann-Jacobsen, K. Opwis, J.S. GutmannAbstractA textile-based reaction system for new peroxidase reactions in non-native media was implemented. The epoxidation of cyclohexene by the commercial peroxidase MaxiBright® was realized with the textile-immobilized enzyme in an adapted liquid-liquid two-phase reactor. A commercially available polyester felt was used as low-price carrier and functionalized with ...
Source: Enzyme and Microbial Technology - January 21, 2020 Category: Biotechnology Source Type: research

A novel green approach for fabricating visible, light sensitive nano-broccoli-like antimony trisulfide by marine Sb(V)-reducing bacteria: revealing potential self-purification in coastal zones
Publication date: Available online 20 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): Haikun Zhang, Jingyi Xie, Yanyu Sun, Ailing Zheng, Xiaoke HuAbstractAntimony trisulfide (Sb2S3) is industrially important for processes ranging from a semiconductor dopant through batteries to a flame retardant. Approaches for fabricating Sb2S3 nanostructures or thin films are by chemical or physicochemical methods, while there have been no report focused on the biological synthesis of nano Sb2S3. In the present study, we fabricated nano-broccoli-like Sb2S3 using Sb(V) reducing bacteria. Thirty four marine and terrestrial st...
Source: Enzyme and Microbial Technology - January 21, 2020 Category: Biotechnology Source Type: research

Taguchi Design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies
This study aimed to statistically optimize the covalent immobilization of Candida rugosa lipase (CRL) onto a ternary support comprised of OPFL derived nanocellulose (NC) and montmorillonite (MMT) in alginate (ALG) (CRL-ALG/NC/MMT). The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT. In addition, amide peaks at 3478 and 1640 cm-1 in the fourier transform infrared spectra affirmed that CRL was covalently bonded to ALG/NC/MMT. The optimized Taguchi Design-a...
Source: Enzyme and Microbial Technology - January 20, 2020 Category: Biotechnology Source Type: research

High level and enantioselective production of L-phenylglycine from racemic mandelic acid by engineered Escherichia coli using response surface methodology
Publication date: Available online 18 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): Cun-Duo Tang, Hong-Ling Shi, Yuan-Yuan Jia, Xiang Li, Lin-Feng Wang, Jian-He Xu, Lun-Guang Yao, Yun-Chao KanAbstractL-Phenylglycine (L-PHG) is a member of unnatural amino acids, and becoming more and more important as intermediate for pharmaceuticals, food additives and agrochemicals. However, the existing synthetic methods for L-PHG mainly rely on toxic cyanide chemistry and multistep processes. To provide green, safe and high enantioselective alternatives, we envisaged cascade biocatalysis for the one-pot synthesis of L-PH...
Source: Enzyme and Microbial Technology - January 20, 2020 Category: Biotechnology Source Type: research

Saturation mutagenesis to improve the degradation of azo dyes by versatile peroxidase and application in form of VP-coated yeast cell walls
Publication date: Available online 15 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): Karla Ilić Đurđić, Raluca Ostafe, Aleksandra Đurđević Đelmaš, Nikolina Popović, Stefan Schillberg, Rainer Fischer, Radivoje ProdanovićAbstractAzo dyes are toxic and carcinogenic synthetic pigments that accumulate as pollutants in aquatic bodies near textile factories. The pigments are structurally diverse, and bioremediation is mostly limited to single dye compounds or related groups. Versatile peroxidase (VP) from Pleurotus eryngii is a heme-containing peroxidase with a broad substrate spectrum that can bre...
Source: Enzyme and Microbial Technology - January 15, 2020 Category: Biotechnology Source Type: research

Characterization of a recombinant L-ribose isomerase from Mycetocola miduiensis and its application for the production of L-ribulose
Publication date: Available online 13 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): Shahid Mahmood, Muhammad Waheed Iqbal, Tahreem Riaz, Hinawi A.M. Hassanin, Yingying Zhu, Dawei Ni, Wanmeng MuAbstractAn enzyme, L-ribose isomerase (L-RI), mostly catalyzes the isomerization of L-ribose and L-ribulose. These so-called rare sugars are essential for the treatment of cancer and other viral diseases. In the present study, L-ribose isomerase produced from a bacterium, Mycetocola miduiensis (Mm-LRIse), by using L-ribose as a carbon source. The recombinant L-ribose isomerase gene was cloned and overexpressed from M....
Source: Enzyme and Microbial Technology - January 15, 2020 Category: Biotechnology Source Type: research

Functional expression of recombinant hybrid enzymes composed of bacterial and insect’s chitinase domains in E. coli
Publication date: Available online 11 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): Aron Paek, Min Jae Kim, Hee Yun Park, Je Geun Yoo, Seong Eun JeongAbstractTo elucidate the functional alteration of the recombinant hybrid chitinases composed of bacterial and insect’s domains, we cloned the constitutional domains from chitinase-encoding cDNAs of a bacterial species, Bacillus thuringiensis (BtChi) and a lepidopteran insect species, Mamestra brassicae (MbChi), respectively, swapped one’s leading signal peptide (LSP) - catalytic domain (CD) - linker region (LR) (LCL) with the other’s chitin b...
Source: Enzyme and Microbial Technology - January 12, 2020 Category: Biotechnology Source Type: research

Elucidation of the decolorization of Congo Red by Trametes versicolor laccase in presence of ABTS through cyclic voltammetry
Publication date: Available online 2 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): Rituparna Saha, Mainak MukhopadhyayAbstractThe azo dye Congo red is heavily used in textile industries and is actively present in the wastewater run-offs. Its structural complexity and physical characteristics make it resistant to the physicochemical treatments employed by the industry. Over time, application of the enzyme laccase has proved to be quite useful due to its ability to oxidize and eventually decolorize the dye. Moreover, the use of ABTS as the electron mediator also helps in enhancing the oxidizing capability of ...
Source: Enzyme and Microbial Technology - January 3, 2020 Category: Biotechnology Source Type: research

Heterologous expression of Deinococcus geothermalis amylosucrase in Corynebacterium glutamicum for luteolin glucoside production
Publication date: Available online 27 December 2019Source: Enzyme and Microbial TechnologyAuthor(s): Young-Wook Chin, Se-Won Jang, Hee-Soon Shin, Tae-Wan Kim, Sun-Ki Kim, Cheon-Seok Park, Dong-Ho SeoAbstractAmylosucrase (ASase) has great industrial potential owing to its multifunctional activities, including transglucosylation, polymerization, and isomerization. In the present study, the properties of Deinococcus geothermalis ASase (DGAS) expressed in Corynebacterium glutamicum (cDGAS) and purified via Ni-NTA affinity chromatography were compared to those of DGAS expressed in Escherichia coli (eDGAS). The pH profile of cDG...
Source: Enzyme and Microbial Technology - December 29, 2019 Category: Biotechnology Source Type: research

Immobilization of recombinant Escherichia coli on multi-walled carbon nanotubes for xylitol production
In this study, recombinant E. coli (rE. coli) was immobilized on untreated and treated multiwalled carbon nanotubes (MWCNTs) for xylitol production. The immobilized rE. coli on untreated MWCNTs gave the highest xylitol production (5.47 g L-1) and a productivity of 0.22 g L-1 h-1. The doubling time for the immobilized cells increased up to 20.40 h and was higher than that of free cells (3.67 h). Cell lysis of the immobilized cells was reduced by up to 73 %, and plasmid stability improved by up to 17 % compared to those of free cells. Xylitol production using the optimum param...
Source: Enzyme and Microbial Technology - December 24, 2019 Category: Biotechnology Source Type: research

Bio-transformation of green tea infusion with tannase and its improvement on adipocyte metabolism
Publication date: April 2020Source: Enzyme and Microbial Technology, Volume 135Author(s): Hae-Soo KIM, Do Yeon JEON, Hafiz Muhammad Ahmad JAVAID, Namood E. SAHAR, Ha-Nul LEE, Seong-Jin HONG, Joo Young HUH, Young-Min KIMAbstractCatechins in green tea possess various health benefits. Enzymatic treatment improves physiological activities by inducing bioconversion of catechins. Here, we investigated the effect of green tea infusion (GT) after tannase treatment, which transforms (-)-epigallocatechin gallate (EGCG) to gallic acid (GA) and (-)-epigallocatechin (EGC), on adipocyte differentiation and mature adipocyte metabolism. T...
Source: Enzyme and Microbial Technology - December 24, 2019 Category: Biotechnology Source Type: research

Zn2+ stapling of N and C-terminal maintains stability and substrate affinity in GH26 endo-mannanase
Publication date: Available online 20 December 2019Source: Enzyme and Microbial TechnologyAuthor(s): Gaurav Singh Kaira, Dandamudi Usharani, Mukesh KapoorAbstractMetal binding sites are present in one-third of proteins and are crucial for biological functions and structural maintenance. GH26 endo-mannanase (ManB-1601) from Bacillus sp. harbors a Zn2+ binding site which connects N (H1, H23) and C (E336)-terminal residues. Present study reveals how native circularization of ManB-1601 through Zn2+ coordination regulates the structure-function. We generated individual Zn2+ coordinating mutants and characterized them using bioc...
Source: Enzyme and Microbial Technology - December 21, 2019 Category: Biotechnology Source Type: research

Construction of Arming Yarrowia lipolytica Surface-displaying Soybean Seed Coat Peroxidase for Use as Whole-cell Biocatalyst
This study also provides a useful strategy for heterologous expression of proteins which could be toxic to intracellular content of host cells. (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - December 21, 2019 Category: Biotechnology Source Type: research

Expression and purification of the full-length N-acetylgalactosaminyltransferase and galactosyltransferase from Campylobacter jejuni in Escherichia coli
In this study, the full-length CgtA43456 (β-(1→4)-N-acetylgalactosaminyltransferase) and CgtB11168 (β-(1→3)-galactosyltransferase) were successfully produced from Escherichia coli through the optimization of E. coli–preferable codon usage, selection of E. coli strain, and use of the molecular chaperone GroEL-GroES (GroEL/ES). The CgtA43456 enzyme was produced as a soluble form in E. coli C41(DE3) co-expressed with codon-optimized CgtA43456 and GroEL/ES. However, soluble CgtB11168 was well expressed in E. coli C41(DE3) with only the codon-optimized CgtB11168. Rather, when co-expressed with GroEL/ES...
Source: Enzyme and Microbial Technology - December 14, 2019 Category: Biotechnology Source Type: research

Efficient synthesis of L-phosphinothricin using a novel aminoacylase mined from Stenotrophomonas maltophilia
This study provides a potential route for the industrial production of L-PPT.Graphical abstract (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - December 14, 2019 Category: Biotechnology Source Type: research

High-level expression of Thermobifida fusca glucose isomerase for high fructose corn syrup biosynthesis
In this study, an efficient expression system for a Thermobifida fusca GIase (GIaseTfus) in Escherichia coli was firstly designed via a two-stage feeding strategy for improving expression level. The cultivation strategy was performed at an exponential feeding rate during the pre-induction phase, followed by a gradient-decreasing feeding rate at the induction phase in a 3-L fermenter. During this process, the effect of induction conditions and the complex nitrogen supplementation in feeding solutions on GIaseTfus production were investigated and optimized. Under the optimal conditions, the yield of GIaseTfus reached 124.1 U...
Source: Enzyme and Microbial Technology - December 14, 2019 Category: Biotechnology Source Type: research

Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms
Publication date: Available online 10 December 2019Source: Enzyme and Microbial TechnologyAuthor(s): Robson Tramontina, Lívia Beatriz Brenelli, Amanda Sousa, Rafael Alves, Ana Maria Zetty Arenas, Viviane Marcos Nascimento, Sarita Cândida Rabelo, Sindélia Freitas, Roberto Ruller, Fabio SquinaAbstractBioproducts production using monomeric sugars derived from lignocellulosic biomass presents several challenges, such as to require a physicochemical pretreatment to improve its conversion yields. Hydrothermal lignocellulose pretreatment has several advantages and results in solid and liquid streams. The forme...
Source: Enzyme and Microbial Technology - December 11, 2019 Category: Biotechnology Source Type: research

Catalytic synthesis of 2,5-bis(hydroxymethyl)furan from 5-hydroxymethylfurfual by recombinant Saccharomyces cerevisiae
Publication date: Available online 11 December 2019Source: Enzyme and Microbial TechnologyAuthor(s): Zhi-Hao Xia, Min-Hua Zong, Ning LiAbstract2,5-Bis(hydroxymethyl)furan (BHMF) is a versatile building block in the synthesis of polymers, fuels, and macrocycle polyethers. In this work, alcohol dehydrogenases (ADHs) were identified from Meyerozyma guilliermondii SC1103 and were heterologously expressed in Saccharomyces cerevisiae for the synthesis of BHMF from 5-hydroxymethylfurfural (HMF). Of recombinant strains constructed, S. cerevisiae expressing an aryl ADH (AAD1669) was observed to be the best catalyst. Upon process op...
Source: Enzyme and Microbial Technology - December 11, 2019 Category: Biotechnology Source Type: research

High-yield and plasmid-free biocatalytic production of 5-methylpyrazine-2-carboxylic acid by combinatorial genetic elements engineering and genome engineering of Escherichia coli
Publication date: Available online 9 December 2019Source: Enzyme and Microbial TechnologyAuthor(s): Liuyan Gu, Haibo Yuan, Xueqin Lv, Guangsheng Li, Rigang Cong, Jianghua Li, Guocheng Du, Long LiuAbstract5-Methylpyrazine-2-carboxylic acid (MPCA) is an important pharmaceutical intermediate and is used in the production of hypoglycemic agents and lipid-lowering drugs. This work aimed to develop a whole-cell biocatalytic process for the efficient synthesis of MPCA from 2, 5-dimethylpyrazine (DMP). Firstly, a whole-cell biocatalyst Escherichia coli strain was constructed by plasmid-based expression of xylene monooxygenase (XMO...
Source: Enzyme and Microbial Technology - December 11, 2019 Category: Biotechnology Source Type: research

Increased Thermal Stability of a Glucose Oxidase Biosensor under High Hydrostatic Pressure
We report the effects of high hydrostatic pressure (HHP), immobilization in electrochemically generated poly-o-phenylenediamine nano-films, and reticulation with glutaraldehyde on the thermal stability of glucose oxidase (GOx). The pseudo-first-order rate constant of inactivation of immobilized GOx inactivated at 70 °C and atmospheric pressure was 20.6 times smaller than that of GOx in solution under the same conditions. Immobilized GOx inactivated at 70 °C and 180 MPa was 87.6 times more stable than GOx in solution inactivated at 70 °C and atmospheric pressure. However, applying hig...
Source: Enzyme and Microbial Technology - December 8, 2019 Category: Biotechnology Source Type: research

Transcriptome analysis of Haematococcus pluvialis of multiple defensive systems against nitrogen starvation
Publication date: Available online 6 December 2019Source: Enzyme and Microbial TechnologyAuthor(s): Yue Zhao, Yuyong Hou, Wenjing Chai, Zhiyong Liu, Xuan Wang, ChunQing He, Zhipeng Hu, Shulin Chen, Weijie Wang, Fangjian chenAbstractHaematococcus pluvialis could accumulate large amounts of triacylglycerol (TAG) and astaxanthin under various environmental stresses. To gain insights into the multiple defensive systems for carbon metabolism against nitrogen starvation, transcriptome analysis was performed. It was found that the genes related to carbon fixation, glycolysis, fatty acid and carotenoid biosynthesis pathways were u...
Source: Enzyme and Microbial Technology - December 8, 2019 Category: Biotechnology Source Type: research

Overexpression of the transcription factor HAC1 improves nerolidol production in engineered yeast
This study provides an effective strategy for sesquiterpene production in yeast. (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - December 4, 2019 Category: Biotechnology Source Type: research

Profiles of plant core-fucosylated N-glycans of acid alpha-glucosidases produced in transgenic rice cell suspension cultures treated with eight different conditions
This study performed the comparison of the N-glycan profiles of rice cell-derived rhGAA to identify the core-fucosylated glycans using UPLC and tandem mass spectrometry. (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - December 1, 2019 Category: Biotechnology Source Type: research

Identification of key sites determining the cofactor specificity and improvement of catalytic activity of a steroid 5β-reductase from Capsella rubella
In this study a steroid 5β-reductase was identified in Capsella rubella (CrSt5βR1) and its function in steroid 5β-reduction was validated experimentally. CrSt5βR1 is capable of enantioselectively reducing the activated C = C bond of broad substrates such as steroids and enones by using NADPH as a cofactor and therefore has the potential as a biocatalyst in organic synthesis. However, for industrial purposes the cheaper NADH is the preferred cofactor. By applying rational design based on literature and complementary mutagenesis strategies, we successfully identified two key amino acid resid...
Source: Enzyme and Microbial Technology - December 1, 2019 Category: Biotechnology Source Type: research

A novel GH30 xylobiohydrolase from Acremonium alcalophilum releasing xylobiose from the non-reducing end
Publication date: Available online 30 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Katarína Šuchová, Vladimír Puchart, Nikolaj Spodsberg, Kristian B.R. Mørkeberg Krogh, Peter BielyAbstractXylanases of the GH30 family are grouped to subfamilies GH30-7 and GH30-8. The GH30-8 members are of bacterial origin and well characterized, while the GH30-7 members are from fungal sources and their properties are quite diverse. Here, a heterologous expression and characterization of the GH30-7 xylanase AaXyn30A from a cellulolytic fungus Acremonium alcalophilum is reported. From vari...
Source: Enzyme and Microbial Technology - December 1, 2019 Category: Biotechnology Source Type: research

Novel approach to produce biomass-derived oligosaccharides simultaneously by recombinant endoglucanase from Trichoderma reesei
Publication date: Available online 27 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Yuheng Tao, Lei Yang, Limin Yin, Chenhuan Lai, Caoxing Huang, Xin Li, Qiang YongAbstractThe recombinant endoglucanase gene (EG I) from Trichoderma reesei was successfully expressed in Pichia pastoris for the purpose of producing oligosaccharides from various biomass-derived substrates. Interestingly, the recombinant endoglucanase I (ReEG I) showed the catalytic activity towards both cellulose and xylan hydrolysis, yet it was more efficient with xylans. Among various glucans and xylans substrates (paper pulp, carboxymethylat...
Source: Enzyme and Microbial Technology - November 29, 2019 Category: Biotechnology Source Type: research

Production of isofloridoside from galactose and glycerol using α-galactosidase from Alicyclobacillus hesperidum
This study provides a possible feasible method for the biosynthesis of isofloridoside from low-cost glycerol and galactose. (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - November 28, 2019 Category: Biotechnology Source Type: research

Enzymatic synthesis and biological characterization of a novel mangiferin glucoside
In this study, novel mangiferin-(1→6)-α-D-glucopyranoside (Mg-G1) was enzymatically synthesized from mangiferin and sucrose using glucansucrase from Leuconostoc mesenteroides B-512F/KM, and optimized using response surface methodology. The water solubility of Mg-G1 was found to be 824.7 mM, which is more than 2,300-fold higher than that of mangiferin. Mg-G1 also showed DPPH radical scavenging activity and superoxide dismutase (SOD)-like scavenging activity, which were 4.77- and 3.71-fold higher than that of mangiferin, respectively. Mg-G1 displayed inhibitory activity against human intestinal maltase and ...
Source: Enzyme and Microbial Technology - November 24, 2019 Category: Biotechnology Source Type: research

Efficient heterologous expression of nicotinate dehydrogenase in Comamonas testosteroni CNB-2 with transcriptional, folding enhancement strategy
Publication date: Available online 22 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Zhen-Hua Lu, Li-Rong Yang, Jian-Ping WuAbstractNicotinate dehydrogenase (NDHase) from Comamonas testosteroni JA1 catalyzes the C6 hydroxylation of 3-cyanopyridine with high regional selectivity, which is a very difficult and complex reaction for chemical synthesis. However, because NDHase is a membrane protein with three subunits (ndhS, ndhL and ndhM), it is difficult to express the enzyme in a functional form using common hosts such as Escherichia coli, Bacilus subtilis or Pichia pastoris. Furthermore, the enzyme re...
Source: Enzyme and Microbial Technology - November 22, 2019 Category: Biotechnology Source Type: research

Autodisplay of alpha amylase from Bacillus megaterium in E. coli for the bioconversion of starch into hydrogen, ethanol and succinic acid
Publication date: Available online 19 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Ana K. Gutiérrez-García, Cecilia Lizeth Alvarez-Guzmán, Antonio De Leon-RodriguezAbstractIn this work, the expression of an α-amylase from Bacillus megaterium on the cell surface of Escherichia coli strains WDHA (Δ hycA and Δ ldhA) and WDHFP (Δ hycA, Δ frdD and Δ pta) by the autodisplay adhesin involved in diffuse adherence (AIDA) system was carried out with the purpose to confer the ability to E. coli strains to degrade starch and thus produce hydrogen, ethanol and s...
Source: Enzyme and Microbial Technology - November 21, 2019 Category: Biotechnology Source Type: research

Expression, biochemical characterization, and mutation of a water forming NADH: FMN oxidoreductase from Lactobacillus rhamnosus
Publication date: Available online 17 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Fei-Long Li, Wen Bin Su, Qing-Lan Tao, Liao-Yuan Zhang, Ye-Wang ZhangAbstractEnzyme-catalyzed cofactor regeneration is a significant approach to avoid large quantities consumption of oxidized cofactor, which is vital in a variety of bioconversion reactions. NADH: FMN oxidoreductase is an ideal regenerating enzyme because innocuous molecular oxygen is required as an oxidant. But the by-product H2O2 limits its further applications at the industrial scale. Here, novel NADH: FMN oxidoreductase (LrFOR) from Lactobacillus rhamnos...
Source: Enzyme and Microbial Technology - November 18, 2019 Category: Biotechnology Source Type: research

Aspergillus niger production of pectinase and α-galactosidase for enzymatic soy processing
Publication date: Available online 17 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Qian Li, Christopher S. Ray, Nicholas V. Callow, Abdullah Al Loman, S.M. Mahfuzul Islam, Lu-Kwang JuAbstractSoybean is a most promising sustainable protein source for feed and food to help meet the protein demand of the rapidly rising global population. To enrich soy protein, the environment-friendly enzymatic processing requires multiple carbohydrases including cellulase, xylanase, pectinase, α-galactosidase and sucrase. Besides enriched protein, the processing adds value by generating monosaccharides that are ready ...
Source: Enzyme and Microbial Technology - November 18, 2019 Category: Biotechnology Source Type: research

Directed evolution of RhlI to generate new and increased quorum sensing signal molecule catalytic activities
Publication date: Available online 16 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Biao Zhang, Lili Ren, Dayong Xu, Haonan Wang, Zhuo Chen, Bingrong Zhang, Xin Zeng, Lianhong Sun, Feng LiAbstractQuorum sensing is a population density-dependent gene expression regulation mechanism in bacteria. The substrate specificity of RhlI, an enzyme in the RhlI–RhlR quorum sensing system of Pseudomonas aeruginosa, was explored by directed evolution to gain insight into the molecular mechanisms of quorum sensing. RhlI catalyzes S-adenosyl methionine and butanoyl or hexanoyl acyl carrier protein to form N-butanoyl...
Source: Enzyme and Microbial Technology - November 16, 2019 Category: Biotechnology Source Type: research

Bio-assay of Acintobacter baumannii using DNA conjugated with gold nano-star: A new platform for microorganism analysis
In this study, gold nano-star was synthesized and used for bio-conjugation with pDNA toward the detection of target sequences. Synthesized probe (5ʹ TTG TGA ACT ATT TAC GTC AGC ATG C3ʹ) of Acinetobacter baumannii was found with excellent sensitivity. After the hybridization of pDNA with cDNA, target DNA (5ʹ GCA TGC TGA CGT AAA TAG TTC ACA A 3ʹ) was easily measured. According to ultra-sensitivity of the engineered optical DNA-based bio-assay, it is potentially applied in the bacterial detection of the environmental and clinical specimens. Here, the selection of engineered biosensor in the presence of two mismatch sequen...
Source: Enzyme and Microbial Technology - November 13, 2019 Category: Biotechnology Source Type: research

Cloning and expression of the Bacillus amyloliquefaciens transglutaminase gene in E. coli using a bicistronic vector construction
Publication date: Available online 12 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Lovaine Silva Duarte, Laísa Quadros Barsé, Pedro Ferrari Dalberto, William Tadeu Santos da Silva, Rafael Costa Rodrigues, Pablo Machado, Luiz Augusto Basso, Cristiano Valim Bizarro, Marco Antônio Záchia AyubAbstractTransglutaminases (TGases) are a class of transferases widely used in the food and biotechnology industries. In this work, we describe the production of recombinant Bacillus amyloliquefaciens TGase in Escherichia coli, obtaining the protein in its soluble and active form. In order to re...
Source: Enzyme and Microbial Technology - November 13, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: January 2020Source: Enzyme and Microbial Technology, Volume 132Author(s): (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - November 13, 2019 Category: Biotechnology Source Type: research

Cutinases as Stereoselective Catalysts: Specific Activity and Enantioselectivity of Cutinases and Lipases for Menthol and its Analogs
Publication date: Available online 12 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): An Su, Serpil Kiokekli, Mariam Naviwala, Abhijit N.Shirke, Ioannis V. Pavlidis, Richard A. GrossAbstractThe specific activity and enantioselectivity of immobilized cutinases from Aspergillus oryzae (AoC) and Humicola insolens (HiC) were compared with those of lipases from Thermomyces lanuginosus (TLL), Rhizomucor miehei (RML) and Lipase B from Candida antarctica (CALB) for menthol and its analogs that include isopulegol, trans-2-tert-butylhexanol (2TBC), and dihydrocarveol (DHC). Common features of these alcohols are two bu...
Source: Enzyme and Microbial Technology - November 12, 2019 Category: Biotechnology Source Type: research