The role of enzyme adsorption in the enzymatic degradation of an aliphatic polyester
Publication date: Available online 21 October 2018Source: Enzyme and Microbial TechnologyAuthor(s): Péter Polyák, Eszter Urbán, Gergely N. Nagy, Beáta G. Vértessy, Béla PukánszkyAbstractThe enzyme catalyzed degradation of poly(3-hydroxybutyrate) (PHB) is a two-step process consisting of the adsorption of the enzyme on the surface of a PHB substrate and the cleavage of ester bonds. A deactivated enzyme was prepared by point mutagenesis to separate the two steps from each other. Measurements carried out with active and inactive enzymes on PHB particles proved that mutagenesis ...
Source: Enzyme and Microbial Technology - October 22, 2018 Category: Biotechnology Source Type: research

Bioprocess optimization for the overproduction of catalytic domain of Ubiquitin-like protease 1 (Ulp1) from S. cerevisiae in E. coli Fed-batch culture
Publication date: Available online 21 October 2018Source: Enzyme and Microbial TechnologyAuthor(s): Babbal, Adivitiya, Shilpa Mohanty, Yogender Pal KhasaAbstractThe exploitation of SUMO (small ubiquitin-related modifier) fusion technology at a large scale for the production of therapeutic proteins with an authentic N-terminus is majorly limited due to the higher cost of ScUlp1 protease. Therefore, the cost-effective production of Saccharomyces cerevisiae Ulp1 protease catalytic domain (402-621 aa) was targeted via its cloning under strong T7 promoter with and without histidine tag. The optimization of cultivation condition...
Source: Enzyme and Microbial Technology - October 21, 2018 Category: Biotechnology Source Type: research

High-efficiency enzymatic production of α-isoquercitrin glucosides by amylosucrase from Deinococcus geothermalis
Publication date: Available online 19 October 2018Source: Enzyme and Microbial TechnologyAuthor(s): Chan-Su Rha, Jung-Min Choi, Young-Sung Jung, Eun-Ryoung Kim, Min Ji Ko, Dong-Ho Seo, Dae-Ok Kim, Cheon-Seok ParkAbstractIsoquercitrin (IQ, quercetin-3-O-β-D-glucopyranoside) has diverse biological functions, such as anti-oxidant and anti-cancer activity, but its use is limited by poor solubility and bioavailability. Enzymatically modified IQ (EMIQ) is a mixture of transglycosylated IQs that have better solubility and bioavailability than do quercetin and IQ. Two different enzymes, cyclodextrin glucanotransferase (CGTase...
Source: Enzyme and Microbial Technology - October 20, 2018 Category: Biotechnology Source Type: research

A single point mutation in hmgA leads to melanin accumulation in Bacillus thuringiensis BMB181
Publication date: Available online 19 October 2018Source: Enzyme and Microbial TechnologyAuthor(s): Tong-tong Tan, Xu-dong Zhang, Zhen Miao, Ying Yu, Si-ling Du, Xiao-yue Hou, Jun CaiAbstractBacillus thuringiensis BMB181 (Bt BMB181), with high melanin production, is an ideal candidate for industrial scale production of light-stable insecticides. However, its melanogenic pathways remain unclear. In the present study, we demonstrated that Bt BMB181 failed to produce melanin after treatment with mesotrione, an inhibitor of 4-hydroxyphenylpyruvate dioxygenase in the homogentisic acid pathway of melanin synthesis. Heterologous ...
Source: Enzyme and Microbial Technology - October 20, 2018 Category: Biotechnology Source Type: research

Bilirubin Oxidase-based Silica Macrocellular Robust Catalyst for On Line Dyes Degradation
This study shows that this biocatalyst is a promising and robust candidate toward wastewater treatments, both in acidic and alkaline conditions where in the latter efficient decolorization strategies were still missing.Graphical Abstract (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - October 12, 2018 Category: Biotechnology Source Type: research

Early biofouling detection using fluorescence-based extracellular enzyme activity
Publication date: Available online 5 October 2018Source: Enzyme and Microbial TechnologyAuthor(s): Babar K. Khan, Luca Fortunato, TorOve LeiknesAbstractMembrane-based filtration technologies have seen rapid inclusion in a variety of industrial processes, especially production of drinking water by desalination. Biological fouling of membranes is a challenge that leads to increased costs from efficiency reductions, membrane damage and ultimately, membrane replacement over time. Such costs can be mitigated by monitoring and optimizing cleaning processes for better prognosis. Monitoring bacterial accumulation in situ can there...
Source: Enzyme and Microbial Technology - October 6, 2018 Category: Biotechnology Source Type: research

Glucansucrase catalyzed synthesis and functional characterization of nordihydroguaiaretic acid glucosides
Publication date: Available online 5 October 2018Source: Enzyme and Microbial TechnologyAuthor(s): Johannes Nolte, Lara-Alina Pöttgen, Julia Sperlich, Alessandra Grossert, Alexander Kempa, Nicole Teusch, Ulrich SchörkenAbstractNordihydroguaiaretic acid (NDGA) is the major lignan of the creosote bush Larrea tridentata known for its antioxidative and pharmacological properties. Here we present the identification of glucansucrases for NDGA glucosylation and the physicochemical and biological characterization of the glucosides. Extracellular glucansucrase of L. pseudomesenteroides DSM 20193 was selected from 19 gluca...
Source: Enzyme and Microbial Technology - October 6, 2018 Category: Biotechnology Source Type: research

Enzymatic Synthesis of Sitagliptin Intermediate Using a Novel ω-Transaminase
In this study, we selected sixteen potential ω -Transaminases (ω -TAs) by BLAST and phylogenetic tree analysis. These ω -TAs were cloned, purified and tested for their reactivity for the synthesis of model β-amino acid (R)-3-amino-4-(2,4,5-triflurophenyl) butanoic acid [3-ATfBA], a key precursor for sitagliptin. In an enzymatic cascade, lipase converted β-ketoester substrate to β-keto acid, which was subsequently aminated by the selected ω -TA to its corresponding β-amino acid. A potent enzyme from Ilumatobacter coccineus (ω -TAIC) was identified for the production of 3-ATf...
Source: Enzyme and Microbial Technology - October 6, 2018 Category: Biotechnology Source Type: research

Functional characterization and comparative analysis of two heterologous endoglucanases from diverging subfamilies of glycosyl hydrolase family 45
Publication date: Available online 27 September 2018Source: Enzyme and Microbial TechnologyAuthor(s): Gabriela Leila Berto, Josman Velasco, Caio Tasso Cabos Ribeiro, Letícia Maria Zanphorlin, Mariane Noronha Domingues, Mario Tyago Murakami, Igor Polikarpov, Leandro Cristante de Oliveira, André Ferraz, Fernando SegatoAbstractLignocellulosic materials are abundant, renewable and are emerging as valuable substrates for many industrial applications such as the production of second-generation biofuels, green chemicals and pharmaceuticals. However, the recalcitrance and the complexity of cell wall polysaccharides r...
Source: Enzyme and Microbial Technology - October 5, 2018 Category: Biotechnology Source Type: research

Xylanase from Aspergillus tamarii shows different kinetic parameters and substrate specificity in the presence of ferulic acid
Publication date: Available online 28 September 2018Source: Enzyme and Microbial TechnologyAuthor(s): Antonielle Viera Monclaro, Guilherme Lima Recalde, Francides Gomes da Silva Jr, Sonia Maria de Freitas, Edivaldo Ximenes Ferreira FilhoAbstractA 22 kDa xylanase (AtXyl1) from Aspergillus tamarii was purified by two chromatographic steps and presented preference for oat spelt (OSX), birchwood (BrX) and beechwood (BeX) xylans respectively, as substrates. AtXyl1 displays the highest activity at pH 5.5 and 55 °C and showed tolerance over a range of different phenolic compounds. The activity of AtXyl1 was not ...
Source: Enzyme and Microbial Technology - October 5, 2018 Category: Biotechnology Source Type: research

Structural characterization and biological evaluation of Staphylosan (dimannooleate), a new glycolipid surfactant produced by a marine Staphylococcus saprophyticus SBPS-15
Publication date: Available online 21 September 2018Source: Enzyme and Microbial TechnologyAuthor(s): Shanmugasundaram Senthil Balan, Panagal Mani, C. Ganesh Kumar, Singaram JayalakshmiAbstractMarine microbes have gained significant attention as potential biofactories for broad spectrum bioactive compounds. In the recent years, bioactive biosurfactants have warranted renewed interest from both environmental and clinical sectors as anti-biofilm agents due to their excellent properties of dispersing microbial biofilms. The present study explores a new glycolipid biosurfactant produced by a marine Staphylococcus saprophyticus...
Source: Enzyme and Microbial Technology - September 22, 2018 Category: Biotechnology Source Type: research

Functional analysis of active amino acid residues of the mercaptosuccinate dioxygenase of Variovorax paradoxus B4
Publication date: Available online 21 September 2018Source: Enzyme and Microbial TechnologyAuthor(s): Ulrike Brandt, Gulsina Galant, Christina Meinert-Berning, Alexander SteinbüchelAbstractThiol dioxygenases are non-heme mononuclear-iron proteins and belong to the cupin superfamily. In 2014, mercaptosuccinate dioxygenase (Msdo) of Variovorax paradoxus B4 was identified as another bacterial cysteine dioxygenase (Cdo) homolog catalyzing the conversion of mercaptosuccinate (MS) into succinate and sulfite. To gain further insights into potentially important amino acid residues for enzyme activity, seven enzyme variants we...
Source: Enzyme and Microbial Technology - September 22, 2018 Category: Biotechnology Source Type: research

A novel low pH fermentation process for the production of acetate and propylene glycol from carbohydrate wastes
Publication date: Available online 21 September 2018Source: Enzyme and Microbial TechnologyAuthor(s): Sathyanarayanan S. Veeravalli, Alexander P. MathewsAbstractA novel low pH fermentation process was studied for the conversion of lactose using Lactobacillus plantarum and Lactobacillus buchneri under anoxic conditions in single co-culture, and two-stage sequential fermentations. This is aimed at producing acetate and propylene glycol (PG) as environmentally benign substitutes for currently used road and aircraft deicing chemicals. The results indicate that in the case of two-stage fermentation with immobilized L. buchneri ...
Source: Enzyme and Microbial Technology - September 22, 2018 Category: Biotechnology Source Type: research

Editorial Board
Publication date: December 2018Source: Enzyme and Microbial Technology, Volume 119Author(s): (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - September 21, 2018 Category: Biotechnology Source Type: research

A facile method to oriented immobilization of His-tagged BirA on Co3+-NTA agarose beads
Publication date: Available online 18 September 2018Source: Enzyme and Microbial TechnologyAuthor(s): Xu Li, Rou Wang, Hanyu Cao, Xu Ting, Lulu Han, Chundong Huang, Lingyun JiaAbstractA facile and economical method was established for the oriented immobilization of biotin ligase (BirA) on Co3+-NTA sepharose through H2O2 oxidation of Co2+ and His-tag. His-tag of the BirA were designed at both N-terminal (His-BirA) and C-terminal (BirA-His), respectively. Immobilization of the His-BirA was performed, realized to 92.85% using by 10 mM H2O2 without compromising catalytic activity. Because amounts of ions on matrix were ...
Source: Enzyme and Microbial Technology - September 19, 2018 Category: Biotechnology Source Type: research

Polymerization of Dopamine Catalyzed by Laccase: Comparison of Enzymatic and Conventional Methods
Publication date: Available online 10 September 2018Source: Enzyme and Microbial TechnologyAuthor(s): Fan Li, Yuanyuan Yu, Qiang Wang, Jiugang Yuan, Ping Wang, Xuerong FanAbstractPolydopamine (PDA) films are generally produced by spontaneous oxidation of dopamine in basic medium, which is a highly convenient method for surface modification. This method is, however, not suitable for alkaline sensitive materials, which have poor stability in alkali medium and need over-long reaction time. Herein, we compared two dopamine polymerization methods: conventional method (induced by O2 at pH 8.5) and laccase-catalyzed method (at pH...
Source: Enzyme and Microbial Technology - September 11, 2018 Category: Biotechnology Source Type: research

Altering the Sensitivity of Escherichia coli Pyruvate Dehydrogenase Complex to NADH Inhibition by Structure-guided Design
In this study, the protein engineering of LPD by structural analysis was explored to eliminate this inhibition. A novel IAA350/351/358VVV triple mutant was successfully verified to be more effective than other LPD mutants reported till date. Notably, PDH activity with the triple mutant at an [NADH]/[NAD+] ratio of 0.15 was still higher than that of the wild-type without NADH addition. The altered enzyme of the PDH complex was also active in the presence of such high NADH levels. This is the first study concerning protein engineering of PDH by structure-guided design. The presence and functional activity of such an NADH-ins...
Source: Enzyme and Microbial Technology - September 6, 2018 Category: Biotechnology Source Type: research

Production of (Z)-11-(heptanoyloxy)undec-9-enoic acid from ricinoleic acid by utilizing crude glycerol as sole carbon source in engineered Escherichia coli expressing BVMO-ADH-FadL
Publication date: Available online 5 September 2018Source: Enzyme and Microbial TechnologyAuthor(s): Pamidimarri D.V.N Sudheer, Dahee Seo, Eun-Joo Kim, Sushma Chauhan, J.R. Chunawala, Kwon-Young ChoiAbstractProduction of (Z)-11-(heptanoyloxy)undec-9-enoic acid from recinoleic acid was achieved by whole-cell biotransformation by Escherichia coli, utilizing crude glycerol as the sole carbon source. Whole-cell biotransformation resulted in ~93 % conversion of the substrate ricinoleic acid to (Z)-11-(heptanoyloxy)undec-9-enoic acid. We replaced the inducer-dependent promoter system (T7 and Rhm promotors) with a constitutive pr...
Source: Enzyme and Microbial Technology - September 6, 2018 Category: Biotechnology Source Type: research

A sensitive and simple method for detecting Cu2+ in plasma using fluorescent Bacillus amyloliquefaciens containing intracellularly biosynthesized CdSe quantum dots
Publication date: Available online 1 September 2018Source: Enzyme and Microbial TechnologyAuthor(s): Zheng-Yu Yan, Cai-Xia Yao, Dong-Yu Wan, Li-Li Wang, Qing-Qing Du, Zhao-Qian Li, Sheng-Mei WuAbstractBacillus amyloliquefaciens containing intracellularly biosynthesized cadmium selenide (CdSe) quantum dots (QDs) was used as a fluorescent bioprobe. Several parameters in the QD biosynthesis process were systematically optimized. The optimized protocol for producing high-quality CdSe QDs in B. amyloliquefaciens features mild synthetic conditions, good reproducibility, short reaction time and high yield. This process shows prom...
Source: Enzyme and Microbial Technology - September 2, 2018 Category: Biotechnology Source Type: research

Screening of novel feruloyl esterases from Talaromyces wortmannii for the development of efficient and sustainable syntheses of feruloyl derivatives
Publication date: Available online 30 August 2018Source: Enzyme and Microbial TechnologyAuthor(s): Io Antonopoulou, Laura Iancu, Peter Jütten, Alexander Piechot, Ulrika Rova, Paul ChristakopoulosABSTRACTThe feruloyl esterases Fae125, Fae7262 and Fae68 from Talaromyces wortmannii were screened in 10 different solvent: buffer systems in terms of residual hydrolytic activity and of the ability for the transesterification of vinyl ferulate with prenol or L-arabinose. Among the tested enzymes, the acetyl xylan-related Fae125 belonging to the phylogenetic subfamily 5 showed highest yield and selectivity for both products in...
Source: Enzyme and Microbial Technology - August 31, 2018 Category: Biotechnology Source Type: research

Cloning and Protein Expression of the sn-1(3) Regioselective Lipase from Cordyceps militaris
In this study, the gene of a novel lipase with sn-1(3) regioselectivity (i.e., sn-1 or sn-3 specific) from Cordyceps militaris was successfully expressed by a heterologous expression system. Total RNA was extracted from C. militaris and then single-stranded cDNA was synthesized. The resulting C. militaris lipase (CML) gene was inserted in Escherichia coli expression plasmids [pET-29b(+), pET-26b, and pColdIII] to construct plasmids encoding CML, which were then transformed to E. coli strains BL21 (DE3), C43 (DE), C41 (DE3), and Origami (DE3) for protein expression. Although the recombinant CML expression level was high, it...
Source: Enzyme and Microbial Technology - August 26, 2018 Category: Biotechnology Source Type: research

Directed evolution of a penicillin V acylase from Bacillus sphaericus to improve its catalytic efficiency for 6-APA production
In this study, a PVA from Bacillus sphaericus (BspPVA) was employed for directed evolution study with hoping to increase its catalytic efficiency. Finally, a triple mutant BspPVA-3 (T63S/N198Y/S110C) was obtained with 12.4-fold specific activity and 11.3-fold catalytic efficiency higher than BspPVA-wt (wild type of BspPVA). Moreover, the conversion yields of 6-APA catalyzed by BspPVA-3 reached 98% with 20% (w/v) penicillin V as substrate, which was significantly higher than that of the BspPVA-wt (85%). Based on the analysis of modeling, the enhancement of specific activity of mutant BspPVA-3 was probably attributed to the ...
Source: Enzyme and Microbial Technology - August 25, 2018 Category: Biotechnology Source Type: research

Kinetic Analysis of Cellulose Synthase of Gluconacetobacter hansenii in Whole Cells and in Purified Form
Publication date: Available online 23 August 2018Source: Enzyme and Microbial TechnologyAuthor(s): John B. McManus, Liza Wilson, Hui Yang, James D. Kubicki, Ming TienAbstractThe Gram-negative bacterium, Gluconacetobacter hansenii, has been long studied and is a model for cellulose synthesis. It produces cellulose, using the enzyme AcsA-AcsB, of exceptionally high crystallinity in comparison to the cellulose of higher plants. We determined the rate of cellulose synthesis in whole cells measured as moles of glucose incorporated into cellulose per second per mole of enzyme. This was determined by quantifying the rate of cellu...
Source: Enzyme and Microbial Technology - August 24, 2018 Category: Biotechnology Source Type: research

Editorial Board
Publication date: November 2018Source: Enzyme and Microbial Technology, Volume 118Author(s): (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - August 23, 2018 Category: Biotechnology Source Type: research

Graphene enhanced transformation of lignin in laccase-ABTS system by accelerating electron transfer
This study could promote the development of novel enzymatic lignin degradation systems coupled with the carbon-based material graphene. (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - August 18, 2018 Category: Biotechnology Source Type: research

Improved thermostability and catalytic efficiency of overexpressed catalase from B. pumilus ML 413 (KatX2) by introducing disulfide bond C286-C289
Publication date: Available online 9 August 2018Source: Enzyme and Microbial TechnologyAuthor(s): Mugisha Samson, Taowei Yang, Mukama Omar, Meijuan Xu, Xian Zhang, Uliho Alphonse, Zhiming RaoAbstractCatalase catalyzes the decomposition of hydrogen peroxide to water and oxygen. The main role of this enzyme is to prevent cell damage caused by reactive oxygen species (ROS). However, endogenous catalase is sensitive to high temperature and possesses limited activity. To satisfy requirements for this critical bottleneck, in this work, we improved the thermo-stability of a heme-catalase (KatX2) from a high oxidative stress resis...
Source: Enzyme and Microbial Technology - August 10, 2018 Category: Biotechnology Source Type: research

Co-immobilized β-galactosidase and Saccharomyces cerevisiae cells for the simultaneous synthesis and purification of galacto-oligosaccharides
Publication date: Available online 9 August 2018Source: Enzyme and Microbial TechnologyAuthor(s): Carla Aburto, Cecilia Guerrero, Carlos Vera, Lorena Wilson, Andrés IllanesAbstractSimultaneous synthesis and purification (SSP) of galacto-oligosaccharides (GOS) from lactose was conducted using a combi-biocatalyst formed by crosslinked enzyme aggregates of Aspergillus oryzae β-galactosidase and Saccharomyces cerevisiae cells co-immobilized by entrapment in calcium alginate gel particles. Product yield obtained with the combi-biocatalyst was similar than obtained with the soluble enzyme (23.3 %), having a final pur...
Source: Enzyme and Microbial Technology - August 10, 2018 Category: Biotechnology Source Type: research

Sustainable Power Generation from Sewage and Energy Recovery from Wastewater with variable resistance using Microbial Fuel Cell
Publication date: Available online 3 August 2018Source: Enzyme and Microbial TechnologyAuthor(s): Debajyoti Bose, Himanshi Dhawan, Vaibhaw Kandpal, P. Vijay, M. GopinathAbstractWastewater from sewage sources contribute significantly to water pollution from domestic waste; one way to recover energy from these sources while at the same time, treating the water is possible using Microbial Fuel Cell. In this work, a two chambered microbial fuel cell was designed and fabricated with carbon cloth electrodes and Nafion-117 membrane, having Platinum as the catalyst. Wastewater from an organic load of 820 ± 30&...
Source: Enzyme and Microbial Technology - August 4, 2018 Category: Biotechnology Source Type: research

Mutational analyses for product specificity of YjiC towards α-mangostin mono-glucoside
This study focuses on a Bacillus licheniformis-originated flexible glycosyltransferase by mutagenesis to examine the active site residues involved in glycosylation for a product specificity towards Mg2. The generated H298A, H298S, and H298C mutants of YjiC exhibited a regiospecificity towards glycosylated product (Mg2) and were targeted in this study. The production pattern of Mg1 decreased to 63 (H298A), 85 (H298S) and 95% (H298C) yields compared to the wild-type YjiC. The increase of uridine 5′-diphosphate (UDP) leading to the inhibition of enzyme activity and production of uridine 5′-diphosphate glucose (UDP...
Source: Enzyme and Microbial Technology - August 4, 2018 Category: Biotechnology Source Type: research

Progress in Using Threonine Aldolases for Preparative Synthesis
Publication date: Available online 30 July 2018Source: Enzyme and Microbial TechnologyAuthor(s): Sarah F. Beaudoin, Michael P. Hanna, Ion Ghiviriga, Jon D. StewartAbstractThree threonine aldolases (TAs) were cloned and overexpressed in Escherichia coli (Aeromonas jandaei L-allo-threonine aldolase, E. coli L-threonine aldolase and Thermotoga maritima L-allo-threonine aldolase). A Design of Experiments strategy was used to identify optimal reaction conditions for each enzyme. These conditions were used to characterize the substrate- and stereoselectivity of each TA toward a panel of aldehyde acceptors. In general, the A. jan...
Source: Enzyme and Microbial Technology - July 31, 2018 Category: Biotechnology Source Type: research

Generation of novel family of reductases from PCR based library for the synthesis of chiral alcohols and amines
In this report, starting from a homology based library, two highly potent whole cell biocatalysts are obtained.Graphical abstract (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - July 28, 2018 Category: Biotechnology Source Type: research

Rational design of a thermophilic β-mannanase fromBacillus subtilis TJ-102 to improve its thermostability
Publication date: November 2018Source: Enzyme and Microbial Technology, Volume 118Author(s): Xiang-Chao Wang, Sheng-Ping You, Jia-Xing Zhang, Ye-Mei Dai, Cheng-Yu Zhang, Wei Qi, Tong-Yi Dou, Rong-Xin Su, Zhi-Min HeAbstractA rational design method to improve β-mannanase (ManTJ102) thermostability was developed successfully in this study. The flexible area of residues 330–340 in ManTJ102 was firstly selected from analysis of molecular dynamics simulation and then the critical amino acid residue (Ala336Pro) with the lowest mutation energy was determined by virtual mutation, whose mutant was named as Mutant336. Afte...
Source: Enzyme and Microbial Technology - July 24, 2018 Category: Biotechnology Source Type: research

Feasibility studies with lignin blocking additives in enhancing saccharification and cellulase recovery: Mutant UV-8 of T. verruculosus IIPC 324 a case study
Publication date: November 2018Source: Enzyme and Microbial Technology, Volume 118Author(s): Lavika Jain, Akhilesh Kumar Kurmi, Deepti AgrawalAbstractThe process economics of fermentable sugar production is dependent on the performance of cellulase cocktail on realistic lignocellulosic biomass and their capability to be recovered and recycled. Feasibility studies were conducted to enhance the digestibility of acid pretreated sugarcane bagasse using novel cellulase cocktail obtained from stable mutant UV-8 of Talaromyces verruculosus IIPC 324 in presence of lignin blocking additives. PEG 6000 was shortlisted as the best add...
Source: Enzyme and Microbial Technology - July 23, 2018 Category: Biotechnology Source Type: research

Editorial Board
Publication date: October 2018Source: Enzyme and Microbial Technology, Volume 117Author(s): (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - July 21, 2018 Category: Biotechnology Source Type: research

Rational design of a thermophilic β-mannanase from Bacillus subtilis TJ-102 to improve its thermostability
Publication date: Available online 19 July 2018Source: Enzyme and Microbial TechnologyAuthor(s): Xiang-Chao Wang, Sheng-Ping You, Jia-Xing Zhang, Ye-Mei Dai, Cheng-Yu Zhang, Wei-Qi, Tong-Yi Dou, Rong-Xin Su, Zhi-Min HeAbstractA rational design method to improve β-mannanase (ManTJ102) thermostability was developed successfully in this study. The flexible area of residues 330-340 in ManTJ102 was firstly selected from analysis of molecular dynamics simulation and then the critical amino acid residue (Ala336Pro) with the lowest mutation energy was determined by virtual mutation, whose mutant was named as Mutant336. Afterw...
Source: Enzyme and Microbial Technology - July 20, 2018 Category: Biotechnology Source Type: research

Production of glutaric acid from 5-aminovaleric acid using Escherichia coli whole cell bio-catalyst overexpressing GabTD from Bacillus subtilis
In this study, as a solution to improve the production of glutaric acid, we introduced gabTD genes from B. subtilis to E. coli for a whole cell biocatalytic approach. This approach enabled us to determine the effect of co-factors on reaction and to achieve a high conversion yield from 5-aminovaleric acid at the optimized reaction condition. Optimization of whole cell reaction by different plasmids, pH, temperature, substrate concentration, and cofactor concentration achieved full conversion with 100 mM of 5-aminovaleric acid to glutaric acid. Nicotinamide adenine dinucleotide phosphate (NAD(P)+) and α-ketoglutaric ...
Source: Enzyme and Microbial Technology - July 11, 2018 Category: Biotechnology Source Type: research

Synergetic effect of Ni2+ and 5-acrylamidobenzoboroxole functional groups anchoring on magnetic nanoparticles for enhanced immobilization of horseradish peroxidase
Publication date: Available online 15 June 2018Source: Enzyme and Microbial TechnologyAuthor(s): Juan Han, Yunfeng Cai, Yun Wang, Lei Gu, Chunmei Li, Yanli Mao, Wenli Zhang, Liang NiAbstractIn this work, a novel Ni2+&AABOB bifunctional core–shell magnetic composite nanoparticles (MNPs), Fe3O4@p(AABOB-co-AIM-Ni2+), were synthesized for the immobilization of horseradish peroxidase (HRP). Two kinds of interaction are simultaneously incorporated to improve the stability and reusability of immobilized HRP: one is the coordination between Ni2+ and amide group in HRP, and the other is the interaction of the wulff-type b...
Source: Enzyme and Microbial Technology - July 10, 2018 Category: Biotechnology Source Type: research

Biochemical characterization of a thermostable cobalt- or copper-dependent polyphenol oxidase with dye decolorizing ability from Geobacillus sp. JS12
Publication date: Available online 28 June 2018Source: Enzyme and Microbial TechnologyAuthor(s): Soo-Jung Moon, Han-Woo Kim, Sung-Jong JeonAbstractA putative laccase-like gene, GPPO, encoding a protein of 17.2 kDa and belonging to the multicopper oxidase family, was cloned and overexpressed in Escherichia coli cells. The purified recombinant protein GPPO is homodecameric protein with a molecular weight of 171.6 kDa. GPPO was not detected the ultraviolet-visible spectroscopy (UV/Vis) spectrum of typical laccases. Co2+ or Cu2+ was essential for substrate oxidation of GPPO, and the enzyme contained 1 mole of Co or Cu pe...
Source: Enzyme and Microbial Technology - July 10, 2018 Category: Biotechnology Source Type: research

Role of electron donor in CO2 fixation of chemoautotrophic bacteria and its preconditions: verification in Alcaligenes hydrogenophilus
Publication date: Available online 28 June 2018Source: Enzyme and Microbial TechnologyAuthor(s): Saiwei Zhang, Ya-nan Wang, Lei Wang, Yiu Fai Tsang, Huan Li, Xiaohua FuAbstractAlcaligenes hydrogenophilus was used to verify the role of the electron donor and acceptor in apparent CO2 fixation of chemoautotrophic bacteria. The response mechanisms underlying the apparent CO2 fixation characteristics with different concentrations of electron donor and acceptor were elucidated by analyzing the transcription characteristics of the cbbL gene, cytoskeleton synthesis efficiency and extracellular free organic carbon concentration. Th...
Source: Enzyme and Microbial Technology - July 10, 2018 Category: Biotechnology Source Type: research

Characterization of an Anaerobic, Thermophilic, Alkaliphilic, High Lignocellulosic Biomass-Degrading Bacterial Community, ISHI-3, Isolated from Biocompost
Publication date: Available online 8 July 2018Source: Enzyme and Microbial TechnologyAuthor(s): Ayumi Shikata, Junjarus Sermsathanaswadi, Thianheng Phakhinee, Sirilak Baramee, Chakrit Tachaapaikoon, Rattiya Waeonukul, Patthra Pason, Khanok Ratanakhanokchai, Akihiko KosugiAbstractThe generation of a complex microbial consortium is a promising approach for efficient biomass decomposition. An anaerobic thermophilic alkaliphilic microbial consortium with efficient degradation ability was screened from bovine manure compost using non-pretreated milling corn stover (CS) and rice straw (RS). A stable microbial consortium ISHI-3 w...
Source: Enzyme and Microbial Technology - July 10, 2018 Category: Biotechnology Source Type: research

Ruminiclostridium josui Abf62A-Axe6A: A tri-functional xylanolytic enzyme exhibiting α-l-arabinofuranosidase, endoxylanase, and acetylxylan esterase activities
In this study, three Abf62A-Axe6A derivatives were constructed, overexpressed in Escherichia coli, purified, and biochemically characterized: RjAbf62A-Axe6A, containing all four modules but lacking the signal peptide; RjAbf62A-CBM6, containing the GH62 and CBM6 modules; and RjAxe6A, containing only CE6. RjAbf62A-Axe6A was highly active toward arabinoxylan and moderately active toward sugar beet arabinan, and released mainly arabinose. Analysis of the arabinoxylooligosaccharide hydrolysis products revealed that RjAbf62A-Axe6A released α-1,2- and α-1,3-linked arabinofuranose from both singly and doubly substitute...
Source: Enzyme and Microbial Technology - July 10, 2018 Category: Biotechnology Source Type: research

N-terminal engineering of overlapping genes in the nitrile hydratase gene cluster improved its activity
Publication date: October 2018Source: Enzyme and Microbial Technology, Volume 117Author(s): Zhengfei Yang, Xiaolin Pei, Gang Xu, Jianping Wu, Lirong YangAbstractNitrile hydratase which catalyzes the hydration of nitriles to the corresponding amides is operon-encoded. However, when heterologously expressed, genes in the same operon are usually not equally expressed, and the ratio needs to be fine-tuned. A gene cluster of three genes (corresponding to α-subunit, β-subunit and activator) encoding the nitrile hydratase was cloned from Aurantimonas manganoxydans ATCC BAA-1229 and expressed in Escherichia coli. Howeve...
Source: Enzyme and Microbial Technology - July 10, 2018 Category: Biotechnology Source Type: research

Gene cloning, expression, and X-ray crystallographic analysis of a β-mannanase from Eisenia fetida
Publication date: October 2018Source: Enzyme and Microbial Technology, Volume 117Author(s): Mitsuhiro Ueda, Yu Hirano, Hiroaki Fukuhara, Yuki Naka, Masami Nakazawa, Tatsuji Sakamoto, Yoshiyuki Ogata, Taro TamadaAbstractThe endo-1,4-β-mannanases (Ef-Man) gene from Eisenia fetida was determined to consist of 1131 bp and encode a 377 amino acid protein. The amino acid sequence showed similarity with the endo-1,4-β-mannanases of Daphnia pulex (62%), Cryptopygus antarcticus (64%), Crassostrea gigas (61%), Mytilus edulis (60%), and Aplysia kurodai (58%). The gene encoding mature Ef-Man was expressed in Pichia pastori...
Source: Enzyme and Microbial Technology - July 10, 2018 Category: Biotechnology Source Type: research

Re-engineering of an Escherichia coli K-12 strain for the efficient production of recombinant human Interferon Gamma
Publication date: October 2018Source: Enzyme and Microbial Technology, Volume 117Author(s): Rajat Pandey, Nitin Kumar, Gabriel A. Monteiro, Venkata Dasu Veeranki, D.M.F. PrazeresAbstractThe Escherichia coli phosphoglucose isomerase (pgi) mutant strain GALG20 was developed previously from wild-type K12 strain MG1655 for increased plasmid yield. To investigate the potential effects of the pgi deletion/higher plasmid levels on recombinant human Interferon Gamma (IFN-γ) production, a detailed network of the central metabolic pathway (100 metabolites, 114 reactions) of GALG20 and MG1655 was constructed. Elementary mode an...
Source: Enzyme and Microbial Technology - July 10, 2018 Category: Biotechnology Source Type: research

In silico modelling and molecular dynamics simulation studies on L-Asparaginase isolated from bacterial endophyte of Ocimum tenuiflorum
Publication date: October 2018Source: Enzyme and Microbial Technology, Volume 117Author(s): Madhuri Pola, Satish Babu Rajulapati, Chandrasai Potla Durthi, Rajeswara Reddy Erva, Mayuri BhatiaAbstractBioactive compounds from endophytes have been used to treat various diseases. In the present study, L-Asparaginase producing endophytes were isolated from Ocimum tenuiflorum (Tulasi) from NIT Warangal, Telangana, India to treat Acute Lymphoblastic Leukemia (ALL) in which L-Asparagine (L-Asn) deamination plays a vital role in ALL treatment. 20 (bacteria and fungi) out of 35 endophytes have been screened for L-Asparaginase product...
Source: Enzyme and Microbial Technology - July 10, 2018 Category: Biotechnology Source Type: research

Carbon-nanotube-caged microbial electrodes for bioelectrocatalysis
Publication date: October 2018Source: Enzyme and Microbial Technology, Volume 117Author(s): Hong-qi Xia, Kento Sakai, Yuki Kitazumi, Osamu Shirai, Kazuyoshi Takagi, Kenji KanoAbstractA method to stably immobilize microbes on electrodes was developed. Resting cells of Methylobacterium extorquens AM1(MeAM1) were caged within multiwalled carbon nanotubes (MWNTs)by adding the cells to a water dispersion of MWNTs then allowing the resulting mixture to dry on electrodes. The MeAM1-MWCNTs electrode thus obtained displayed excellent activities in the bidirectional bioelectrocatalysis due to formate dehydrogenase(s) in the resting ...
Source: Enzyme and Microbial Technology - July 10, 2018 Category: Biotechnology Source Type: research

Immobilization of β-galactosidase and α-mannosidase onto magnetic nanoparticles: A strategy for increasing the potentiality of valuable glycomic tools for glycosylation analysis and biological role determination of glycoconjugates
Publication date: October 2018Source: Enzyme and Microbial Technology, Volume 117Author(s): Ernesto Rodríguez, Karen Francia, Natalie Brossard, Juan J. García Vallejo, Hakan Kalay, Yvette van Kooyk, Teresa Freire, Cecilia GiacominiAbstractGlycans present in biological glycoconjugates have several structural and functional roles. Elucidation of glycan structure and biological function is critical to understand their role in physiological and pathogenic process, enabling the development of diagnostic methods and disease treatment. Immobilized glycosidases are powerful tools for glycan analysis, as they are able...
Source: Enzyme and Microbial Technology - July 10, 2018 Category: Biotechnology Source Type: research

Synthesis of conjugated linoleic acid-rich triacylglycerols by immobilized mutant lipase with excellent capability and recyclability
In this study, CLA-rich TAG were successfully synthesized by an immobilized mutant lipase (MAS1-H108A)-catalyzed esterification of CLA-rich fatty acids and glycerol under vacuum. MAS1-H108A was first immobilized onto ECR1030 resin. Results showed that the lipase/support ratio of 41 mg/g was suitable for the immobilization and the thermostability of immobilized MAS1-H108A was greatly enhanced. Subsequently, the immobilized MAS1-H108A was employed for the synthesis of CLA-rich TAG and 95.21% TAG with 69.19% CLA was obtained under the optimized conditions. The TAG content (95.21%) obtained by immobilized MAS1-H108A is the r...
Source: Enzyme and Microbial Technology - July 10, 2018 Category: Biotechnology Source Type: research

A kinetic spectrophotometric method for the determination of pyridoxal-5′-phosphate based on coenzyme activation of apo-d-phenylglycine aminotransferase
Publication date: October 2018Source: Enzyme and Microbial Technology, Volume 117Author(s): Juntratip Jomrit, Duangnate Isarangkul, Pijug Summpunn, Suthep WiyakruttaAbstractA new PLP assay method based on the coenzyme activation of apo-d-phenylglycine aminotransferase (apo-d-PhgAT) is reported. The assay process is comprised of two steps. First, PLP present in plasma samples is allowed to reconstitute apo-d-PhgAT, forming active holo-d-PhgAT. In the second step, the enzymatic activity of reconstituted d-PhgAT is determined using d-4-OH-phenylglycine as the amino donor substrate with 4-OH-benzoylformate (OH-BZF) as the reac...
Source: Enzyme and Microbial Technology - July 10, 2018 Category: Biotechnology Source Type: research

Quorum quenching bacteria encapsulated in PAC-PVA beads for enhanced membrane antifouling properties
Publication date: October 2018Source: Enzyme and Microbial Technology, Volume 117Author(s): Zhuotong Zeng, Bi Tang, Rong Xiao, Jinhui Huang, Yanling Gu, Yahui Shi, Yi Hu, Jianxin Zhou, Hua Li, Lixiu Shi, Guangming ZengAbstractIn order to improve the antifouling properties of quorum quenching (QQ) bacteria immobilized beads, the mechanical strength and permeability of QQ beads were modified by adding powdered activated carbon (PAC) based on traditional polyvinyl alcohol (PVA)-boric acid method. Optimal PAC concentration was investigated through measuring the mechanical strength, permeability and N-octanoyl-DLhomoserine lact...
Source: Enzyme and Microbial Technology - July 10, 2018 Category: Biotechnology Source Type: research