Enhanced laccase activity of biocatalytic hybrid copper hydroxide nanocages
Publication date: Available online 17 May 2019Source: Enzyme and Microbial TechnologyAuthor(s): Omar Silva-Torres, Luis Bojorquez-Vazquez, Andrey Simakov, Rafael Vazquez-DuhaltAbstractNanobiocatalysis is the combination of the unique properties of nano-sized materials and the efficiency and sophistication of catalytic properties of enzymes. In this work, Cu(OH)2 nanocages with an mean size of 170 nm were synthesized and used as a support for the covalent conjugation with fungal ligninolytic enzymes; versatile peroxidase and laccase. Both enzymes have the ability to degrade a wide range of pollutants. The nanocages w...
Source: Enzyme and Microbial Technology - May 18, 2019 Category: Biotechnology Source Type: research

Immobilization of high temperature-resistant GH3 β-glucosidase on a magnetic particle Fe3O4-SiO2-NH2-Cellu-ZIF8/zeolitic imidazolate framework
Publication date: Available online 14 May 2019Source: Enzyme and Microbial TechnologyAuthor(s): Xuejia Shi, Jin Xu, Changning Lu, Zhenzhong Wang, Wei Xiao, Linguo ZhaoAbstractThe magnetic particle Fe3O4-SiO2-NH2-Cellu-ZIF8 (FSNCZ8) was used to immobilize the high-temperature resistant GH3 β-glucosidase (Tpebgl3) from the Thermomotoga petrophila DSM 13995. The Tpebgl3 has great potential in the catalytic conversion of pharmaceutically active components. The magnetic carrier (FSNCZ8) which provided stronger adsorption capacity, magnetic and high-temperature stability than the previously discussed Fe3O4-NH2-Cellu-...
Source: Enzyme and Microbial Technology - May 16, 2019 Category: Biotechnology Source Type: research

Application of Acetyl-CoA Synthetase from Methanothermobacter thermautotrophicus to Non-Native Substrates
Publication date: Available online 14 May 2019Source: Enzyme and Microbial TechnologyAuthor(s): Louis M.M. Mouterde, Jon D. StewartAbstractThe substrate selectivity of the Trp416Gly mutant of Methanothermobacter thermautotrophicus acetyl-CoA synthetase (Trp416Gly MT-ACS1) was explored. The goal was to identify its substrate range, particularly for functionalized carboxylic acid substrates that would allow post-synthesis functionalization of CoA thioesters or downstream products using metathesis or Click chemistry. Relative activities were determined by in situ formation of acyl-hydroxamate iron (III) complexes. Trp416Gly M...
Source: Enzyme and Microbial Technology - May 16, 2019 Category: Biotechnology Source Type: research

Novel glycosylated human interferon alpha 2b expressed in glycoengineered Pichia pastoris and its biological activity: N-linked glycoengineering approach
Publication date: Available online 15 May 2019Source: Enzyme and Microbial TechnologyAuthor(s): Srikanth Katla, K.N.R. Yoganand, Smita Hingane, C.T. Ranjith Kumar, B. Anand, Senthilkumar SivaprakasamAbstractHuman interferon alpha 2b (IFN α2b) is a type I interferon exhibiting antiviral, anti-proliferative and immunomodulatory activities. The clinical outcome of the approved recombinant human IFN α2b drugs in the market suffers from short plasma half-life, rapid clearance and other side effects. Human IFN α2b expression in mammalian cell lines results in significant heterogeneity in glycan moieties, incons...
Source: Enzyme and Microbial Technology - May 16, 2019 Category: Biotechnology Source Type: research

Preparation and characterization of endolysin-containing liposomes and evaluation of their antimicrobial activities against Gram-negative bacteria
Publication date: Available online 15 May 2019Source: Enzyme and Microbial TechnologyAuthor(s): Jaewoo Bai, Eunhye Yang, Pahn-shick Chang, Sangryeol RyuABSTRACTThe overuse and misuse of antibiotics in treating bacterial infections cause the rapid emergence of drug-resistant bacteria, suggesting that the development of alternative strategies to control antibiotic-resistant bacteria is urgently needed. Endolysins are bacteriophage-encoded enzymes that can degrade peptidoglycan in bacterial cell walls, and they have great potential as alternative antimicrobial agents. However, exogenous application of recombinant endolysin is...
Source: Enzyme and Microbial Technology - May 16, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: August 2019Source: Enzyme and Microbial Technology, Volume 127Author(s): (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - May 12, 2019 Category: Biotechnology Source Type: research

Tubes for detection of cholinesterase inhibitors—Unique effects of Neusilin on the stability of butyrylcholinesterase-impregnated carriers
In this study, the stability of our patented carriers in the form of pellets with immobilized butyrylcholinesterase containing an increasing amount of the unique sorbent Neusilin® US2 was evaluated. The samples containing Neusilin maintained the stability of the immobilized enzyme for a longer time even at higher temperature and humidity than the currently commercially used carrier without Neusilin, allowing improved detection of nerve agents.Graphical abstract (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - May 12, 2019 Category: Biotechnology Source Type: research

Production of glutaric acid from 5-aminovaleric acid by robust whole-cell immobilized with polyvinyl alcohol and polyethylene glycol
In this study, we sought to enhance the stability and reusability of this whole-cell system for realizing the efficient production of glutaric acid under harsh reaction conditions. To this end, various matrices were screened to immobilize Escherichia coli whole-cell overexpressing 4-aminobutyrate aminotransferase (GabT), succinate semi-aldehyde dehydrogenase (GabD), and NAD(P)H oxidase (NOX). We ultimately selected a PVA-PEG gel (LentiKats®) for cell entrapment, and several factors of the reaction were optimized. The optimal temperature and pH were 35 °C and 8.5, respectively. Treatment with Tween 80 as a su...
Source: Enzyme and Microbial Technology - May 10, 2019 Category: Biotechnology Source Type: research

Tubes for detection of cholinesterase inhibitors – unique effects of Neusilin on the stability of butyrylcholinesterase-impregnated carriers
In this study, the stability of our patented carriers in the form of pellets with immobilized butyrylcholinesterase containing an increasing amount of the unique sorbent Neusilin® US2 was evaluated. The samples containing Neusilin maintained the stability of the immobilized enzyme for a longer time even at higher temperature and humidity than the currently commercially used carrier without Neusilin, allowing improved detection of nerve agents.Graphical abstract (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - May 7, 2019 Category: Biotechnology Source Type: research

Utilization of enzyme extract self-encapsulated within Polypyrrole in sensitive detection of catechol
This study employs polyphenol oxidase extract, biosynthesized by white-rot fungi Trametes pubescens (TP) for the development of amperometric biosensors for catechol detection. The catalytic activity of the crude extract was firstly used to induce the bio-synthesis of conducting polymer - polypyrrole (Ppy), resulting in the self-encapsulation of the enzyme extract within conducting material - Ppy. The viability and biological integrity of the enzyme extract was preserved after the synthesis and was able to efficiently detect phenolic compounds such as catechol. Comparative evaluations between the biosynthesized Ppy based bi...
Source: Enzyme and Microbial Technology - May 4, 2019 Category: Biotechnology Source Type: research

Synthesis of catalase-inorganic hybrid nanoflowers via sonication for colorimetric detection of hydrogen peroxide
Publication date: Available online 3 May 2019Source: Enzyme and Microbial TechnologyAuthor(s): Miaorong Zhang, Na Yang, Yuxiang Liu, Jianguo TangAbstractA catalase-inorganic hybrid nanoflowers (Cat-HNFs)-based colorimetric platform was prepared for visual detection of hydrogen peroxide (H2O2). Catalase via coordination was self-assembled and embedded in the copper phosphate crystals by sonication. Under the designed colorimetric system, Cat-HNFs showed linear absorbance responses toward H2O2 in the concentration range of 1 μM to 1 mM, and the detection limit was 1 μM. Compared to free catalase, Cat-HNFs exhibited enh...
Source: Enzyme and Microbial Technology - May 4, 2019 Category: Biotechnology Source Type: research

Preparation, functionalization and characterization of rice husk silica for lipase immobilization via adsorption
Publication date: Available online 3 May 2019Source: Enzyme and Microbial TechnologyAuthor(s): Natália B. Machado, João P. Miguez, Iara C.A. Bolina, Adriana B. Salviano, Raphael A.B. Gomes, Olga L. Tavano, Jaine H.H. Luiz, Paulo W. Tardioli, Érika C. Cren, Adriano A. MendesAbstractSilica has been extracted from rice husks via a simple hydrothermal process and functionalized with triethoxy(octyl)silane – OCTES (Octyl–SiO2) and (3-aminopropyl)triethoxysilane – 3-APTES (Amino–SiO2), with the aim of using it as support to immobilize lipase from Thermomyces lanuginosus (TLL) via adso...
Source: Enzyme and Microbial Technology - May 4, 2019 Category: Biotechnology Source Type: research

Surface display of sialyltransferase on the outer membrane of Escherichia coli and ClearColi
In this study PmST1 was expressed on the outer membrane of wildtype Escherichia coli (BL21) with lipopolysaccharide (LPS) and ClearColi with no LPS, and then the enzyme activity and expression level of PmST1 were compared. As the first step, the expression levels of PmST1 on the outer membranes of wildtype E. coli (BL21) and ClearColi were compared according to the IPTG induction time, and the absolute amount of surface-displayed PmST1 was calculated using densitometry of SDS-PAGE. As the next step, the influence of LPS on the PmST1 activity was estimated by analyzing Michaelis-Menten plot. The enzyme activity of PmST1 was...
Source: Enzyme and Microbial Technology - May 2, 2019 Category: Biotechnology Source Type: research

Preparation of diisononyl adipate in a solvent-free system via an immobilized lipase-catalyzed esterification
In this study, diisononyl adipate was synthesized from adipic acid and isononyl alcohol in a solvent-free system via immobilized lipase-catalyzed esterification. Liquid Eversa lipase from Thermomyces lanuginosus was immobilized on Lewatit VP OC 1600 carrier. The effects of temperature, substrate molar ratio, water activity of enzyme, vacuum, and enzyme loading on the reaction efficiency were investigated. Application of vacuum played a key role to achieve a 100% conversion. The optimal temperature, molar ratio (adipic acid to isononyl alcohol), water activity of enzyme, vacuum were 50 °C, 1:3, 0.75, 13.3 ...
Source: Enzyme and Microbial Technology - May 1, 2019 Category: Biotechnology Source Type: research

Production of D-glucuronic acid from myo-inositol using Escherichia coli whole-cell biocatalyst overexpressing a novel myo-inositol oxygenase from Thermothelomyces thermophile
This study provides a feasible method for the industrial production of GlcUA. (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - April 25, 2019 Category: Biotechnology Source Type: research

Identification of peptide inhibitors of matrix metalloproteinase 1 using an in-house assay system for the enzyme
Publication date: Available online 22 April 2019Source: Enzyme and Microbial TechnologyAuthor(s): Moon Won Min, Chae-Eun Kim, Sushma Chauhan, Hyeon Ji Park, Chang-Seo Park, Tae Hyeon Yoo, Taek Jin KangAbstractMatrix metalloproteinases (MMPs) are zinc-dependent proteases involved in the degradation of extracellular matrix proteins. As one of the isoforms, MMP-1 breaks down collagen, and its activity is known to be important in wound healing. Its timely and adequate level of expression is pivotal because MMP-1 is also involved in the damage or aging of skins as well as in certain types of cancers. Thus, both assaying the MMP...
Source: Enzyme and Microbial Technology - April 24, 2019 Category: Biotechnology Source Type: research

Kraton based polymeric nanocomposite bioanode for the application in a biofuel cell
The objective of this work is to introduce a nanocomposite based bioanode for biofuel cell application. The as-prepared Kraton/MWCNTs nanocomposite was used as a current enhancer and ferritin (Frt) as a mediator between glucose oxidase (GOx) and the electrode surface. The hybrid anode achieved enzymatic oxidation of glucose by the nanocomposite reflecting an efficient energy conversion from glucose. The resulting Kraton/MWCNTs/Frt/GOx bioande exhibited good catalytic activity towards glucose oxidation along with excellent stability. The maximum current density attained by the bioanode is 1.14 mA cm−2 at the op...
Source: Enzyme and Microbial Technology - April 20, 2019 Category: Biotechnology Source Type: research

Inhibitory effects of CuInS2 and CdTe nanoparticles on macrophage cytokine production and phagocytosis in vitro
Publication date: Available online 17 April 2019Source: Enzyme and Microbial TechnologyAuthor(s): Cai-Xia Yao, Tian-Yang Lin, Yi-Long Su, Hui Zou, Zheng-Yu Yan, Sheng-Mei WuAbstractMacrophages eliminate and destroy invading bacteria and contaminants by engulfing them or secreting cytokines that trigger downstream immune responses. Consequently, impairment of the phagocytic functions of macrophages and/or suppressing their cytokine secretion are dangerous to organisms that rely on immune protection. Accordingly, exposure to environmental nanoparticles (NPs) that display immunomodulatory properties are serious. In this work,...
Source: Enzyme and Microbial Technology - April 19, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: July 2019Source: Enzyme and Microbial Technology, Volume 126Author(s): (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - April 17, 2019 Category: Biotechnology Source Type: research

Insight into kinetics and thermodynamics of a novel hyperstable GH family 10 endo-1,4-β-xylanase (TnXynB) with broad substrates specificity cloned from Thermotoga naphthophilaRKU-10T
Publication date: Available online 14 April 2019Source: Enzyme and Microbial TechnologyAuthor(s): Ikram ul Haq, Fatima AkramAbstractCurrently, hyperstable endo-1,4-β-xylanase has been the focus of attention as potent biocatalyst as well as utilization in bioconversion process. Therefore, the gene (1,035 bp) of a monomeric glycoside hydrolase family 10 (GH10) endo-1,4-β-xylanase (TnXynB) from a hyperthermophilic eubacterium Thermotoga naphthophila RKU-10T was cloned and overexpressed in a mesophilic host system. The extracellular TnXynB was purified to homogeneity with a molecular mass of 40 kDa, and showed...
Source: Enzyme and Microbial Technology - April 16, 2019 Category: Biotechnology Source Type: research

Enhanced production of cadaverine by the addition of hexadecyltrimethylammonium bromide to whole cell system with regeneration of pyridoxal-5′-phosphate and ATP
In this study, we introduced an ATP regeneration system using polyphosphate kinase (ppk) into systems containing cadaverine decarboxylase (CadA) and PdxY for a sufficient supply of PLP, which resulted in enhanced cadaverine production. In addition, to improve transport efficiency, the use of surfactants was tested. We found that membrane permeabilization via hexadecyltrimethylammonium bromide (CTAB) increased the yield of cadaverine in the presence of high concentrations of L-lysine. By combining these two strategies, the ppk system and addition of CTAB, we enhanced cadaverine production up to 100% with 1 M of L-lys...
Source: Enzyme and Microbial Technology - April 16, 2019 Category: Biotechnology Source Type: research

Sodium azide intervention, salinity stress and two-step cultivation of Dunaliella tertiolecta for lipid accumulation
Publication date: Available online 12 April 2019Source: Enzyme and Microbial TechnologyAuthor(s): Hao-Hong Chen, Lu-Lu Xue, Ming-Hua Liang, Jian-Guo JiangAbstractA two-step strategy was employed to culture Dunaliella tertiolecta, an oleaginous unicellular green algae, combined by the salt stress and sodium azide intervention, to observe their effects on its lipid accumulation. When the algae cultured at different salt concentrations reached the logarithmic growth phase, sodium azide was added. The results showed that the addition of sodium azide significantly increased the lipid content and had no significant effect on cel...
Source: Enzyme and Microbial Technology - April 13, 2019 Category: Biotechnology Source Type: research

Effects of triethylamine on the expression patterns of two G3PDHs and lipid accumulation in Dunaliella tertiolecta
This study aims to investigate the effect of triethylamine on GPDH expression. Results showed that, when treated by triethylamine at 100 ppm and 150 ppm, the expression levels of Dtgdp1 and Dtgpd2 were increased to 5.121- and 56.964-fold compared with the control, respectively. Triethylamine seemed to enhance lipid metabolic flow by inducing the expressions of Dtgdp1 and Dtgdp2 to increase the lipid content, which provides a new insight into the desired pathway of lipid synthesis in algae through genetic engineering. (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - April 10, 2019 Category: Biotechnology Source Type: research

Functional exploration of Pseudoalteromonas atlantica as a source of hemicellulose-active enzymes: evidence for a GH8 xylanase with unusual mode of action
Publication date: Available online 8 April 2019Source: Enzyme and Microbial TechnologyAuthor(s): Sayani Ray, Jacqueline Vigouroux, Axelle Bouder, Mathilde Francin Allami, Audrey Geairon, Mathieu Fanuel, David Ropartz, William Helbert, Marc Lahaye, Estelle BonninAbstractTo address the need for efficient enzymes exhibiting novel activities towards cell wall polysaccharides, the bacterium Pseudoalteromonas atlantica was selected based on the presence of potential hemicellulases in its annotated genome. It was grown in the presence or not of hemicelluloses and the culture filtrates were screened towards 42 polysaccharides. P. ...
Source: Enzyme and Microbial Technology - April 10, 2019 Category: Biotechnology Source Type: research

Rational design to improve activity of the Est3563 esterase from Acinetobacter sp. LMB-5
In this study, computer-aided simulation mutagenesis was used to improve the esterase activity with a tightened screening library and enlarged success rate. Two positive mutants, P218R and A242R, were obtained with 2.5 and 2.1 folds higher than the WT Est3563 esterase, with 11.96 ± 0.45 U·mg-1 and 9.90 ± 0.52 U·mg-1, respectively. With the help of bioinformatics analysis and three-dimensional printing technology, it was found that the mutations could increase the 240-280 residues swing distance and make them deviate from the catalytic pocket. The instability and devia...
Source: Enzyme and Microbial Technology - April 7, 2019 Category: Biotechnology Source Type: research

Mutagenesis on the surface of a β-agarase from Vibrio sp. ZC-1 increased its thermo-stability
Publication date: Available online 6 April 2019Source: Enzyme and Microbial TechnologyAuthor(s): Bing-Mei Su, Xin-Qi Xu, Ren-Xiang Yan, Yong Xie, Juan LinAbstractThe recombinant rAgaZC-1 was a family GH50 β-agarase from Vibrio sp. ZC-1 (CICC 24670). In this paper, the mutant D622G (i.e., mutate the aspartic acid at position 622 to glycine) had better thermo-stability than rAgaZC-1, showing 1.5℃ higher T5010 (the temperature at which the half-time is 10 minutes) and 4-folds of half-time at 41℃, while they had almost same optimum temperature (38.5℃), optimum pH (pH6.0) and catalytic efficiency. Thermal deactivatio...
Source: Enzyme and Microbial Technology - April 7, 2019 Category: Biotechnology Source Type: research

Kraton based polymeric nanocomposites bioanode for the application in a biofuel cell
The objective of this work is to introduce a nanocomposite based bioanode for biofuel cell application. The as-prepared Kraton/MWCNTs nanocomposite was used as a current enhancer and ferritin (Frt) as a mediator between glucose oxidase (GOx) and the electrode surface. The hybrid anode achieved enzymatic oxidation of glucose by the nanocomposite reflecting an efficient energy conversion from glucose. The resulting Kraton/MWCNTs/Frt/GOx bioande exhibited good catalytic activity towards glucose oxidation along with excellent stability. The maximum current density attained by the bioanode is 1.14 mA cm-2 at the optimum ...
Source: Enzyme and Microbial Technology - April 5, 2019 Category: Biotechnology Source Type: research

Recombinant expression and molecular insights into the catalytic mechanism of an NADPH-dependent conjugated polyketone reductase for the asymmetric synthesis of (R)-pantolactone
In this study, we investigated an alternative method to prepare (R)-pantolactone through asymmetric reduction of ketopantolactone (KPL). An NADPH-dependent conjugated polyketone reductase gene from Candida dubliniensis CD36 (CduCPR) was functionally overexpressed in Escherichia coli BL21 (DE3). Recombinant CduCPR belonged to the aldo-keto reductase superfamily, and showed high catalytic activity and stereoselectivity using KPL as the substrate. In a continuous feeding reaction, 200 mM ketopantolactone was reduced to (R)-pantolactone with 98% conversion and 99% enantiomeric excess (e.e.) within 2.0 h. The cata...
Source: Enzyme and Microbial Technology - April 4, 2019 Category: Biotechnology Source Type: research

Fusion of the N-terminal domain of Pseudomonas sp. phytase with Bacillus sp. phytase and its effects on optimal temperature and catalytic efficiency
In this study, BPP produced by Bacillus sp. SJ-10 (SJ-10phy) was fused with the N-terminal domain of PSphy and the enzymatic properties of the resulting fusion protein (FUSJ-10phy) were investigated. FUSJ-10phy exhibited an optimal temperature that was 10 °C lower than that of the wild-type enzyme. A comparison of kinetic parameters showed that the turnover rate of FUSJ-10phy was 2.39-fold higher than that of SJ-10phy, representing a 1.79-fold increase in catalytic efficiency. In addition, BPP produced by Bacillus sp. SJ-48 has relatively low sequence similarity with SJ-10phy, was fused with N-terminal domain (F...
Source: Enzyme and Microbial Technology - April 4, 2019 Category: Biotechnology Source Type: research

A Thermostable Chitinase from the Antagonistic Chromobacterium violaceum that Inhibits the Development of Phytopathogenic Fungi
Publication date: Available online 1 April 2019Source: Enzyme and Microbial TechnologyAuthor(s): Antônio J.S. Sousa, Christiana F.B. Silva, Jeanlex S. Sousa, José E. Monteiro-Júnior, José E.C. Freire, Bruno L. Sousa, Marina D.P. Lobo, Ana C.O. Monteiro-Moreira, Thalles B. GrangeiroAbstractThe biocontrol activity of some soil strains of Chromobacterium sp. against pathogenic fungi has been attributed to secreted chitinases. The aim of this work was to characterize biochemically a recombinant chitinase (CvChi47) from C. violaceum ATCC 12472 and to investigate its effects on phytopathogenic fungi. C...
Source: Enzyme and Microbial Technology - April 2, 2019 Category: Biotechnology Source Type: research

Enhancing thermostability of Yarrowia lipolytica lipase 2 through engineering multiple disulfide bonds and mitigating reduced lipase production associated with disulfide bonds
In this study, combining different regional disulfide bonds had greater effect than combining same regional disulfide bonds. Furthermore, mutants with 4, 5, and 6 disulfide bonds exhibited dramatically enhanced thermostability. Compared with the wild-type, sextuple mutant 6s displayed a 22.53 and 31.23 ℃ increase in the melting temperature (Tm) and the half loss temperature at 15 min (T15 50), respectively, with greater pH stability and a wider reaction pH range. Molecular dynamics simulation revealed that multiple disulfide bonds resulted in more rigid structures of mutants 4s, 5s and 6s, and prolonged enzyme unfolding ...
Source: Enzyme and Microbial Technology - April 2, 2019 Category: Biotechnology Source Type: research

High efficiency biotransformation of bisphenol A in a fluidized bed reactor using stabilized laccase in porous silica
Publication date: Available online 28 March 2019Source: Enzyme and Microbial TechnologyAuthor(s): Mingyue Piao, Donglei Zou, Xianghao Ren, Song Gao, Chuanyu Qin, Yunxian PiaoAbstractWe developed a high efficiency bisphenol A (BPA) treating system in a fluidized bed reactor (FBR) using stabilized laccase (Lac) in mesoporous silica (SER/Lac). The SER/Lac, prepared by cross linking of each enzymes inside the porous silica using glutaraldehyde, presented improved stability than the free Lac over various pH and temperatures with shaking. When the SER/Lac was used for BPA biotransformation in a batch reaction, higher efficiency ...
Source: Enzyme and Microbial Technology - March 30, 2019 Category: Biotechnology Source Type: research

Effects of divalent copper on microbial community, enzymatic activity and functional genes associated with nitrification and denitrification at tetracycline stress
Publication date: Available online 25 March 2019Source: Enzyme and Microbial TechnologyAuthor(s): Zhiwei Deng, Zichao Wang, Pan Zhang, Pinghui Xia, Kedong Ma, Degang Zhang, Lin Wang, Yusuo Yang, Yuejing Wang, Shipeng Chen, Shilong DengAbstractThe effects of divalent copper (Cu(II)) on microbial community, enzymatic activity and functional genes in a sequencing batch reactor (SBR) at tetracycline (TC) stress were investigated. The enzymatic activity and functional genes abundance associated with nitrification and denitrification at a 20 mg L-1 TC stress were higher than those at a mixtures stress of 20 ...
Source: Enzyme and Microbial Technology - March 27, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: June 2019Source: Enzyme and Microbial Technology, Volume 125Author(s): (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - March 16, 2019 Category: Biotechnology Source Type: research

Manipulating intradiol dioxygenases by C-terminus truncation
Publication date: June 2019Source: Enzyme and Microbial Technology, Volume 125Author(s): Ali Reza Nazmi, Muralidharan Muthu, Gareth Lloyd-JonesAbstractIntradiol dioxygenases (EC 1.13.11.1) are bacterial enzymes that catalyze the ring cleavage of catechols which is a central step in the aerobic degradation of aromatic compounds. Some members of this enzyme group have a C-terminus which is 4–5% longer (an additional 13–18 amino acids) compared to the majority of known sequences. The longer C-terminus itself is not highly conserved and appears to be poorly integrated in the protein structural models developed for ...
Source: Enzyme and Microbial Technology - March 10, 2019 Category: Biotechnology Source Type: research

Structure and catalytic mechanistic insight into Enterobacter aerogenes acetolactate decarboxylase
Publication date: Available online 5 March 2019Source: Enzyme and Microbial TechnologyAuthor(s): Fangling Ji, Yanbin Feng, Mingyang Li, Feida Long, Yongliang Yang, Tianqi Wang, Jingyun Wang, Yongming Bao, Song XueAbstractα-Acetolactate decarboxylase (ALDC) catalyses α-acetolactate into acetoin (3-hydroxy-2-butanone, AC) and is considered to be the rate-limiting enzyme in the synthesis of 2,3-butanediol. In this work, the enzymatic activity of ALDC from Enterobacter aerogenes ALDC (E.a.-ALDC) was fully characterized with enzyme kinetics, indicating a Km of 14.83 ± 0.87 mM and a kcat o...
Source: Enzyme and Microbial Technology - March 6, 2019 Category: Biotechnology Source Type: research

Enhanced lipase-catalyzed synthesis of sugar fatty acid esters using supersaturated sugar solution in ionic liquids
Publication date: Available online 4 March 2019Source: Enzyme and Microbial TechnologyAuthor(s): Dong Woo Shin, Ngoc Lan Mai, Sang-Woo Bae, Yoon-Mo KooAbstractA solvent-mediated method (SMM) was used to prepare supersaturated sugar solutions in hydrophobic and mixture of hydrophilic/hydrophobic ionic liquids (ILs), namely, [Bmim][Tf2N] and [Bmim][TfO]/[Bmim][Tf2N], respectively. In this method, sugars were first solubilized in a mixture of organic solvent and water (i.e. methanol:water, 1:1 v/v), and then added to [Bmim][Tf2N] and/or [Bmim][TfO]/[Bmim][Tf2N] mixture. Supersaturated sugar solution in ILs were obtained by re...
Source: Enzyme and Microbial Technology - March 5, 2019 Category: Biotechnology Source Type: research

Identification of a novel carboxypeptidase encoded by Rv3627c that plays a potential role in Mycobacteria morphology and cell division
Publication date: Available online 4 March 2019Source: Enzyme and Microbial TechnologyAuthor(s): Wenli Zhang, Sheng Li, Li Ma, Yuefei Xu, Wenyong DingAbstractFunctionally uncharacterized gene Rv3627c is predicted to encode a carboxypeptidase in the pathogen of Mycobacterium tuberculosis (M. tuberculosis), which remains a major threat to human health. Here, we sought to reveal the function of Rv3627c and to elucidate its effects on mycobacterial growth. Rv3627c was purified from E. coli using Ni2+-NTA affinity chromatography, and its identity was confirmed with a monoclonal anti-polyhistidine antibody. An enzyme activity as...
Source: Enzyme and Microbial Technology - March 5, 2019 Category: Biotechnology Source Type: research

Cells-on-Nanofibers: Effect of polyethyleneimine on hydrophobicity of poly-Ɛ-caprolacton electrospun nanofibers and immobilization of bacteria
In conclusion, PCL-PEI/G.oxydans whole-cell biosensor was tested for sensing of glucose as a model analyte.Graphical abstract (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - March 5, 2019 Category: Biotechnology Source Type: research

Structure-guided engineering of ChKRED20 from Chryseobacterium sp. CA49 for asymmetric reduction of aryl ketoesters
Publication date: Available online 4 March 2019Source: Enzyme and Microbial TechnologyAuthor(s): Tong-Biao Li, Feng-Jiao Zhao, Zhongchuan Liu, Yun Jin, Yan Liu, Xiao-Qiong Pei, Zhi-Gang Zhang, Ganggang Wang, Zhong-Liu WuAbstractChKRED20 is a robust NADH-dependent ketoreductase identified from the genome of Chryseobacterium sp. CA49 that can use 2-propanol as the ultimate reducing agent. The wild-type can reduce over 100 g/l ketones for some pharmaceutical relevant substrates, exhibiting a remarkable potential for industrial application. In this work, to overcome the limitation of ChKRED20 to aryl ketoesters, we firs...
Source: Enzyme and Microbial Technology - March 5, 2019 Category: Biotechnology Source Type: research

Strategy and regulatory mechanisms of glutamate feeding to enhance astaxanthin yield in Xanthophyllomyces dendrorhous
Publication date: Available online 28 February 2019Source: Enzyme and Microbial TechnologyAuthor(s): Baobei Wang, Xueshan Pan, Jing Jia, Weide Xiong, Emmanuel Manirafasha, Xueping Ling, Lu YinghuaAbstractXanthophyllomyces dendrorhous is an excellent industrial source for production of natural astaxanthin, but the yield of astaxanthin is relative low due to the contradiction between biomass weight and astaxanthin accumulation. Glutamate, a metabolite connecting nitrogen and carbon metabolisms, is probably a promising entry point to interfere cellular metabolisms. Thus, the effect of glutamate on cell growth and astaxanthin ...
Source: Enzyme and Microbial Technology - March 1, 2019 Category: Biotechnology Source Type: research

Carbohydrate-binding property of a cell wall integrity and stress response component (WSC) domain of an alcohol oxidase from the rice blast pathogen Pyricularia oryzae
Publication date: Available online 25 February 2019Source: Enzyme and Microbial TechnologyAuthor(s): Shinichi Oide, Yuya Tanaka, Akira Watanabe, Masayuki InuiAbstractThe cell wall integrity and stress response component (WSC) domain was first described in the Wsc-family protein of the yeast Saccharomyces cerevisiae, and later found in diverse eukaryotic organisms. Due solely to their presence in the Wsc-family proteins working as a plasma membrane sensor for surface stress and in a fungal β-1,3-exoglucanse, WSC domains have been presumed to possess carbohydrate-binding property without any experimental evidence. Aimin...
Source: Enzyme and Microbial Technology - February 26, 2019 Category: Biotechnology Source Type: research

A novel D-2-hydroxy acid dehydrogenase with high substrate preference for phenylpyruvate originating from lactic acid bacteria: structural analysis on the substrate specificity
This study suggests a structural basis for the high substrate preference of the 2-HADH, and further engineering possibilities to synthesize versatile 2-hydroxy acids. (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - February 25, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: May 2019Source: Enzyme and Microbial Technology, Volume 124Author(s): (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - February 21, 2019 Category: Biotechnology Source Type: research

Manipulating intradiol dioxygnenases by C-terminus truncation
Publication date: Available online 19 February 2019Source: Enzyme and Microbial TechnologyAuthor(s): Ali Reza Nazmi, Muralidharan Muthu, Gareth Lloyd-JonesAbstractIntradiol dioxygenases (EC 1.13.11.1) are bacterial enzymes that catalyze the ring cleavage of catechols which is a central step in the aerobic degradation of aromatic compounds. Some members of this enzyme group have a C-terminus which is 4-5% longer (an additional 13-18 amino acids) compared to the majority of known sequences. The longer C-terminus itself is not highly conserved and appears to be poorly integrated in the protein structural models developed for ...
Source: Enzyme and Microbial Technology - February 20, 2019 Category: Biotechnology Source Type: research

Site-directed mutation to improve the enzymatic activity of 5-carboxy-2-pentenoyl-CoA reductase for enhancing adipic acid biosynthesis
In this study, the pathway producing adipate via a reverse reaction of degradation pathway of adipic acid was ported from Thermobifida fusca to Escherichia coli (E. coli). The pathway contains 6 genes: Tfu_0875, Tfu_2399, Tfu_0067, Tfu_1647, Tfu_2576 and Tfu_2577, which encodes β-ketothiolase, 3-hydroxyacyl-CoA dehydrogenase, 3-hydroxyadipyl-CoA dehydrogenase, 5-carboxy-2-pentenoyl-CoA reductase and adipyl-CoA synthetase, respectively. Of the genes in this pathway, Tfu_1647 is the limited step.Here, we constructed a homology model of 5-carboxy-2-pentenoyl-CoA reductase and found that Lys295 and Glu334 were the active ...
Source: Enzyme and Microbial Technology - February 17, 2019 Category: Biotechnology Source Type: research

Significantly improved catalytic efficiency of caffeic acid O-methyltransferase towards N-acetylserotonin by strengthening its interactions with the unnatural substrate’s terminal structure
Publication date: Available online 14 February 2019Source: Enzyme and Microbial TechnologyAuthor(s): Wenya Wang, Sisi Su, Shizhuo Wang, Lidan Ye, Hongwei YuAbstractO-methylation of N-acetylserotonin (NAS) has been identified as the bottleneck in melatonin biosynthesis pathway. In the present paper, caffeic acid O-methyltransferase from Arabidopsis thaliana (AtCOMT) was engineered by rational design to improve its catalytic efficiency in conversion of NAS to melatonin. Based on the notable difference in the terminal structure of caffeic acid and NAS, mutants were designed to strengthen the interactions between the substrate...
Source: Enzyme and Microbial Technology - February 15, 2019 Category: Biotechnology Source Type: research

Corrigendum to “N-terminal domain of the beta-propeller phytase of Pseudomonas sp. FB15 plays a role for retention of low-temperature activity and catalytic efficiency” [Enzyme Microb. Technol. 117 (October) (2018) 84–90]
Publication date: Available online 10 February 2019Source: Enzyme and Microbial TechnologyAuthor(s): Won Je Jang, Jong Min Lee, Hae Dae Park, Yu Bin Choi, In-Soo Kong (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - February 11, 2019 Category: Biotechnology Source Type: research

Cloning and expression of a β-mannanase gene from Bacillus sp. MK-2 and its directed evolution by random mutagenesis
Publication date: Available online 7 February 2019Source: Enzyme and Microbial TechnologyAuthor(s): Wen Zhang, Zhemin Liu, Sijia Zhou, Haijin Mou, Ruifu ZhangAbstractA β-mannanase gene was cloned from Bacillus sp. MK-2 and expressed in Bacillus subtilis WB800. The ORF of the β-mannanase gene was 1104 bp in length, encoding 367 aa. The deduced amino acid sequence shared high sequence identity with the β-mannanase from Bacillus subtilis, and belongs to glycosyl hydrolase family 26. The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C...
Source: Enzyme and Microbial Technology - February 8, 2019 Category: Biotechnology Source Type: research

Biochemical characterization of peptidylarginine deiminase-like orthologs from thermotolerant Emericella dentata and Aspergillus nidulans
Publication date: Available online 7 February 2019Source: Enzyme and Microbial TechnologyAuthor(s): Ashraf S.A. El-Sayed, Ahmed A. Shindia, Azza A. AbouZaid, Amany M. Yassin, Gul Shad Ali, Mahmoud Z. SitohyAbstractPeptidylarginine deiminases (PADs) are a group of hydrolases, mediating the deimination of peptidylarginine residues into peptidyl-citrulline. Equivocal protein citrullination by PADs of fungal pathogens has a strong relation to the progression of multiple human diseases, however, the biochemical properties of fungal PADs remain ambiguous. Thus, this is the first report exploring the molecular properties of PAD f...
Source: Enzyme and Microbial Technology - February 8, 2019 Category: Biotechnology Source Type: research