Manipulating intradiol dioxygnenases by C-terminus truncation
Publication date: Available online 19 February 2019Source: Enzyme and Microbial TechnologyAuthor(s): Ali Reza Nazmi, Muralidharan Muthu, Gareth Lloyd-JonesAbstractIntradiol dioxygenases (EC 1.13.11.1) are bacterial enzymes that catalyze the ring cleavage of catechols which is a central step in the aerobic degradation of aromatic compounds. Some members of this enzyme group have a C-terminus which is 4-5% longer (an additional 13-18 amino acids) compared to the majority of known sequences. The longer C-terminus itself is not highly conserved and appears to be poorly integrated in the protein structural models developed for ...
Source: Enzyme and Microbial Technology - February 20, 2019 Category: Biotechnology Source Type: research

Site-directed mutation to improve the enzymatic activity of 5-carboxy-2-pentenoyl-CoA reductase for enhancing adipic acid biosynthesis
In this study, the pathway producing adipate via a reverse reaction of degradation pathway of adipic acid was ported from Thermobifida fusca to Escherichia coli (E. coli). The pathway contains 6 genes: Tfu_0875, Tfu_2399, Tfu_0067, Tfu_1647, Tfu_2576 and Tfu_2577, which encodes β-ketothiolase, 3-hydroxyacyl-CoA dehydrogenase, 3-hydroxyadipyl-CoA dehydrogenase, 5-carboxy-2-pentenoyl-CoA reductase and adipyl-CoA synthetase, respectively. Of the genes in this pathway, Tfu_1647 is the limited step.Here, we constructed a homology model of 5-carboxy-2-pentenoyl-CoA reductase and found that Lys295 and Glu334 were the active ...
Source: Enzyme and Microbial Technology - February 17, 2019 Category: Biotechnology Source Type: research

Significantly improved catalytic efficiency of caffeic acid O-methyltransferase towards N-acetylserotonin by strengthening its interactions with the unnatural substrate’s terminal structure
Publication date: Available online 14 February 2019Source: Enzyme and Microbial TechnologyAuthor(s): Wenya Wang, Sisi Su, Shizhuo Wang, Lidan Ye, Hongwei YuAbstractO-methylation of N-acetylserotonin (NAS) has been identified as the bottleneck in melatonin biosynthesis pathway. In the present paper, caffeic acid O-methyltransferase from Arabidopsis thaliana (AtCOMT) was engineered by rational design to improve its catalytic efficiency in conversion of NAS to melatonin. Based on the notable difference in the terminal structure of caffeic acid and NAS, mutants were designed to strengthen the interactions between the substrate...
Source: Enzyme and Microbial Technology - February 15, 2019 Category: Biotechnology Source Type: research

Corrigendum to “N-terminal domain of the beta-propeller phytase of Pseudomonas sp. FB15 plays a role for retention of low-temperature activity and catalytic efficiency” [Enzyme Microb. Technol. 117 (October) (2018) 84–90]
Publication date: Available online 10 February 2019Source: Enzyme and Microbial TechnologyAuthor(s): Won Je Jang, Jong Min Lee, Hae Dae Park, Yu Bin Choi, In-Soo Kong (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - February 11, 2019 Category: Biotechnology Source Type: research

Cloning and expression of a β-mannanase gene from Bacillus sp. MK-2 and its directed evolution by random mutagenesis
Publication date: Available online 7 February 2019Source: Enzyme and Microbial TechnologyAuthor(s): Wen Zhang, Zhemin Liu, Sijia Zhou, Haijin Mou, Ruifu ZhangAbstractA β-mannanase gene was cloned from Bacillus sp. MK-2 and expressed in Bacillus subtilis WB800. The ORF of the β-mannanase gene was 1104 bp in length, encoding 367 aa. The deduced amino acid sequence shared high sequence identity with the β-mannanase from Bacillus subtilis, and belongs to glycosyl hydrolase family 26. The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C...
Source: Enzyme and Microbial Technology - February 8, 2019 Category: Biotechnology Source Type: research

Biochemical characterization of peptidylarginine deiminase-like orthologs from thermotolerant Emericella dentata and Aspergillus nidulans
Publication date: Available online 7 February 2019Source: Enzyme and Microbial TechnologyAuthor(s): Ashraf S.A. El-Sayed, Ahmed A. Shindia, Azza A. AbouZaid, Amany M. Yassin, Gul Shad Ali, Mahmoud Z. SitohyAbstractPeptidylarginine deiminases (PADs) are a group of hydrolases, mediating the deimination of peptidylarginine residues into peptidyl-citrulline. Equivocal protein citrullination by PADs of fungal pathogens has a strong relation to the progression of multiple human diseases, however, the biochemical properties of fungal PADs remain ambiguous. Thus, this is the first report exploring the molecular properties of PAD f...
Source: Enzyme and Microbial Technology - February 8, 2019 Category: Biotechnology Source Type: research

Engineered thermostable β–fructosidase from Thermotoga maritima with enhanced fructooligosaccharides synthesis
Publication date: Available online 6 February 2019Source: Enzyme and Microbial TechnologyAuthor(s): Menéndez Carmen, Martínez Duniesky, R.Pérez Enrique, Musacchio Alexis, Ramírez Ricardo, López-Munguía Agustín, Hernández LázaroAbstractThe thermostable β–fructosidase (BfrA) from the bacterium Thermotoga maritima converts sucrose into glucose, fructose, and low levels of short–chain fructooligosaccharides (FOS) at high substrate concentration (1.75 M) and elevated temperatures (60–70 °C). In this research, FOS produced by ...
Source: Enzyme and Microbial Technology - February 7, 2019 Category: Biotechnology Source Type: research

Effect of Intense Pulsed Light on the Deactivation of Lipase: Enzyme-deactivation Kinetics and Tertiary Structural Changes by Fragmentation
Publication date: Available online 5 February 2019Source: Enzyme and Microbial TechnologyAuthor(s): Min-Sik Jeon, Kyung-Min Park, Hyunjong Yu, Jun-Young Park, Pahn-Shick ChangAbstractThe effect of intense pulsed light (IPL) irradiation on Chromobacterium viscosum lipase was investigated with a primary focus on catalytic activity and molecular structure. During IPL irradiation, lipase activity decreased significantly with increasing pulse fluence (Fp) and exposure time (te). IPL-induced deactivation kinetics were further elucidated based on a two-step series-type deactivation model (constant deactivation rate k1> k2). Fp...
Source: Enzyme and Microbial Technology - February 6, 2019 Category: Biotechnology Source Type: research

Hydrogen-bond-based protein engineering for the acidic adaptation of Bacillus acidopullulyticus pullulanase
In this study, we conducted protein engineering to improve the acidic adaptation of Bacillus acidopullulyticus pullulanase (BaPul) and used a hydrogen-bond-based approach to identify promising residues that may change the deprotonation constants (pKa) of the catalytic residues. A total of 19 amino acids were selected for mutation according to the crystal structure of BaPul. The pH optimum of the L627R mutant shifted from 5.0 to 4.0, and its relative activity at pH 4.0 was 117% that of the wide-type enzyme. The improved efficacy of the L627R mutant at pH 4.0 was confirmed by kinetic parameters and pKa prediction. Moreover, ...
Source: Enzyme and Microbial Technology - January 31, 2019 Category: Biotechnology Source Type: research

The modular arabinanolytic enzyme Abf43A-Abf43B-Abf43C from Ruminiclostridium josui consists of three GH43 modules classified in different subfamilies
Publication date: Available online 30 January 2019Source: Enzyme and Microbial TechnologyAuthor(s): Makiko Sakka, Kazunobu Yamada, Taichi Kitamura, Emi Kunitake, Tetsuya Kimura, Kazuo SakkaABSTRACTThe abnA gene from Ruminiclostridium josui encodes the large modular arabinanolytic enzyme, Abf43 A-Abf43B-Abf43C, consisting of an N-terminal signal peptide, a Laminin_G_3 module, a GH43_22 module, a Laminin_G_3 module, a Big_4 module, a GH43_26 module, a GH43_34 module and a dockerin module in order with a calculated molecular weight of 204,108. Three truncated enzymes were recombinantly produced in Escherichia coli and ...
Source: Enzyme and Microbial Technology - January 31, 2019 Category: Biotechnology Source Type: research

Molecular engineering of chitinase from Bacillus sp. DAU101 for enzymatic production of chitooligosaccharides
Publication date: Available online 30 January 2019Source: Enzyme and Microbial TechnologyAuthor(s): Mengyan Pan, Jianghua Li, Xueqin Lv, Guocheng Du, Long LiuAbstractEnzymatic production of chitooligosaccharides has high value in medicine and other fields. However, low chitinase activity and yield of chitooligosaccharides limit the production and application. Herein, we used a series of molecular biology strategies to increase the expression of chitinase in Bacillus subtilis WB600. Upon addition of the signal peptide NprB, Chisb was successfully secreted to the outside of the cell and extracellular expression level reached...
Source: Enzyme and Microbial Technology - January 31, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: April 2019Source: Enzyme and Microbial Technology, Volume 123Author(s): (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - January 25, 2019 Category: Biotechnology Source Type: research

Producing alcohol and salt stress tolerant strain of Saccharomyces cerevisiae by heterologous expression of pprI gene
ConclusionExpression of pprI in different cells increases the tolerance toward various compounds including ethanol, salt and butanol along with boosted yield of biofuel production from ligonocellulosic substrate. Mutant strains showed a higher capability of producing alcohol, and cellular tolerance was raised toward growth restricting compounds released from substrates. (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - January 24, 2019 Category: Biotechnology Source Type: research

Effects of lipopolysaccharide structure on lycopene production in Escherichia coli
In this study, the effects of lipopolysaccharides with different structures on lycopene biosynthesis were investigated. Firstly, the heterogenous crtEBI operon were overexpressed in 10 LPS mutant strains of E. coli W3110 (ΔwaaC, ΔwaaF, ΔwaaY, ΔwaaG, ΔwaaR, ΔwaaO, ΔwaaU, ΔwaaP, ΔwaaY and ΔwaaB), and their ability to produce lycopene were compared. ΔwaaC/pWSK29-crtEBI, ΔwaaF/pWSK29-crtEBI and ΔwaaY/pWSK29-crtEBI produced 4.19, 4.20, and 3.81 mg/g lycopene, respectively, while the control W3110/pWSK29-crtEBI produced 3.71 mg/g lycopene...
Source: Enzyme and Microbial Technology - January 24, 2019 Category: Biotechnology Source Type: research

Xerogel based catalyst for improved cathode performance in microbial fuel cells
Publication date: Available online 17 January 2019Source: Enzyme and Microbial TechnologyAuthor(s): Bhim Sen Thapa, S. Seetharaman, Raghuram Chetty, T.S ChandraAbstractIn a microbial fuel cell (MFC) the reduction reaction at cathode has been a limiting factor for achieving maximum power density, and numerous strategies were attempted to overcome this. Herein, we demonstrate carbon xerogel (CX) doped with iron (Fe) and nitrogen (N) followed by modification with graphene oxide (GO) as an efficient catalyst for MFC. The CXFeNGO catalyst was characterized by scanning electron microscope, X-ray diffraction and the catalytic act...
Source: Enzyme and Microbial Technology - January 18, 2019 Category: Biotechnology Source Type: research

An improved enzyme nanoparticles based amperometric pyruvate biosensor for detection of pyruvate in serum
Publication date: Available online 11 January 2019Source: Enzyme and Microbial TechnologyAuthor(s): Mansi Malik, Reeti Chaudhary, Chandra Shekhar PundirAbstractThe nanoparticles of commercially available pyruvate oxidase (POx) from Aerococcus species were prepared by desolvation method, which were then characterized and covalently immobilized onto gold electrode (AuE) to construct an improved model of amperometric pyruvate biosensor. The POxNPs/Au electrode was analyzed morphologically by scanning electron microscopy (SEM). On the other hand, cyclic voltammetry studies (CV) and electrochemical impedance spectroscopy (EIS) ...
Source: Enzyme and Microbial Technology - January 11, 2019 Category: Biotechnology Source Type: research

Protein engineering of Pseudomonas fluorescens peroxidase Dyp1B for oxidation of phenolic and polymeric lignin substrates
Publication date: Available online 9 January 2019Source: Enzyme and Microbial TechnologyAuthor(s): Rahman Rahman Pour, Austine Ehibhatiomhan, Yuling Huang, Ben Ashley, Goran M. Rashid, Sharon Mendel-Williams, Timothy D.H. BuggAbstractDirected evolution was applied to dye-decolourizing peroxidase Dyp1B from Pseudomonas fluorescens Pf-5, in order to enhance the activity for oxidation of phenolic and lignin substrates. Saturation mutagenesis was used to generate focused libraries at 7 active site residues in the vicinity of the heme cofactor, and the libraries were screened for activity towards 2,6-dichlorophenol. Mutants N19...
Source: Enzyme and Microbial Technology - January 10, 2019 Category: Biotechnology Source Type: research

Editorial Board
Publication date: March 2019Source: Enzyme and Microbial Technology, Volume 122Author(s): (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - January 10, 2019 Category: Biotechnology Source Type: research

Comparison of two models of surface display of xylose reductase in the Saccharomyces cerevisiae cell wall
Publication date: Available online 6 January 2019Source: Enzyme and Microbial TechnologyAuthor(s): Amir Sk. Hossain, Renata Teparić, Vladimir MršaAbstractIn order to display xylose reductase at the surface of S. cerevisiae cells two different gene constructs have been prepared. In the first, xylose reductase gene GRE3 was fused with two parts of the CCW12 gene, the N-terminal one coding for the secretion signal sequence, and the C–terminal coding for the glycosylphosphatidylinositol anchoring signal. Transformed cells synthesized xylose reductase and incorporated it in the cell wall through the remnant of the...
Source: Enzyme and Microbial Technology - January 6, 2019 Category: Biotechnology Source Type: research

Identification of enzyme(s) capable of degrading endosulfan and endosulfan sulfate using in silico techniques
Publication date: Available online 4 January 2019Source: Enzyme and Microbial TechnologyAuthor(s): Ngangbam Sarat Singh, Ranju Sharma, Dileep K. SinghAbstractEndosulfan is one of the most widely used organochlorine cyclodiene insecticides. Microbial oxidation of endosulfan forms endosulfan sulfate, which is more or less equally toxic and persistent as endosulfan. Due to lack of specificity and efficiency of microbial bioremediation technique in the field conditions, enzymatic bioremediation is receiving huge attention to clean-up the environment. In the present study x-ray crystal structures of enzymes from Brookhaven Prot...
Source: Enzyme and Microbial Technology - January 5, 2019 Category: Biotechnology Source Type: research

A simplified method to remove fusion tags from a xylanase of Bacillus sp. HBP8 with HRV 3C protease
Publication date: Available online 4 January 2019Source: Enzyme and Microbial TechnologyAuthor(s): Hu Xu, Qian Wang, Zhiwei Zhang, Li Yi, Lixin Ma, Chao ZhaiAbstractHuman rhinovirus 3C protease (HRV 3C protease) is commonly used as a tool to remove fusion tags from recombinant proteins in gene engineering due to its distinguished specificity and high activity at low temperature. This paper is aimed to simplify the strategy of removing epitope tags from target proteins with HRV 3C protease. Fusion proteins composed of a xylanase from Bacillus sp. HBP8 (xylHB) and double tags (MBP/Nus and 6×His, with an HRV 3C protease...
Source: Enzyme and Microbial Technology - January 5, 2019 Category: Biotechnology Source Type: research

A mechanized urease activity assay
Publication date: Available online 3 January 2019Source: Enzyme and Microbial TechnologyAuthor(s): Justyna Bzura, Robert KonckiAbstractTwo fully mechanized flow analysis systems for urease activity assays have been developed, characterized and compared. Both of them are based on almost the same compact system of solenoid micropumps and microvalves controlled and actuated by highly effective, low-power and economic Arduino microcontroller. For photometric detection of ammonia formed in the course of enzymatic hydrolysis of urea, the Berthelot method and the Nessler reaction have been examined. For both these detection schem...
Source: Enzyme and Microbial Technology - January 4, 2019 Category: Biotechnology Source Type: research

Biosynthesis of (deoxy)guanosine-5′-triphosphate by GMP kinase and acetate kinase fixed on the surface of E. coli
In this study, we investigated the application of whole-cell catalysts constructed by bacterial cell surface display in biosynthetic reactions of 5′-(d)GTP from (deoxy)guanosine-5′-monophosphate (5′-(d)GMP). By N-terminal or N- and C-terminal fusion of the ice nucleation protein, we successfully displayed the GMP kinase of Lactobacillus bulgaricus and the acetate kinase of E. coli on the surface of E. coli cells. A large amount of soluble target protein was obtained upon induction with 0.2 mM IPTG at 25 °C for 30 h. The conversion of dGMP was up to 91% when catalysed by the surfac...
Source: Enzyme and Microbial Technology - January 4, 2019 Category: Biotechnology Source Type: research

Biosynthesis of (deoxy)guanosine-5’-triphosphate by GMP kinase and acetate kinase fixed on the surface of E. coli
In this study, we investigated the application of whole-cell catalysts constructed by bacterial cell surface display in biosynthetic reactions of 5’-(d)GTP from (deoxy)guanosine-5’-monophosphate (5’-(d)GMP). By N-terminal or N- and C-terminal fusion of the ice nucleation protein, we successfully displayed the GMP kinase of Lactobacillus bulgaricus and the acetate kinase of E. coli on the surface of E. coli cells. A large amount of soluble target protein was obtained upon induction with 0.2 mM IPTG at 25 °C for 30 h. The conversion of dGMP was up to 91% when catalysed by the surfac...
Source: Enzyme and Microbial Technology - December 28, 2018 Category: Biotechnology Source Type: research

Development of strategy for simultaneous enhanced production of alkaline xylanase-pectinase enzymes by a bacterial isolate in short submerged fermentation cycle
Publication date: Available online 21 December 2018Source: Enzyme and Microbial TechnologyAuthor(s): Divya Sharma, Geeta Sharma, Ritu MahajanAbstractThe aim of this study is to enhance the production of industrially valuable xylanase and pectinase enzymes in short duration, using agrowaste extracted substrates. Conventional cum statistical multifactor analysis approaches were used in order to evaluate the effect of crude extracted substrates, supplemented for the production of xylanase-pectinase enzymes. Incorporation of crude extracted xylan (1.2 mg/ml of inoculum) and pectin (4.8 mg/ml of inoculum) substrat...
Source: Enzyme and Microbial Technology - December 22, 2018 Category: Biotechnology Source Type: research

The effect of an oligosaccharide reducing-end xylanase, BhRex8A, on the synergistic degradation of xylan backbones by an optimised xylanolytic enzyme cocktail
Publication date: Available online 19 December 2018Source: Enzyme and Microbial TechnologyAuthor(s): Samkelo Malgas, Brett. I. PletschkeAbstractXylan, the most abundant hemicellulose in lignocellulosic biomass, requires a consortium of xylanolytic enzymes to achieve its complete de-polymerisation. As global interest in using xylan containing lignocellulosic feedstocks for biofuel production increases, an accompanying knowledge on how to efficiently depolymerize these feedstocks into fermentable sugars is required. Since it has been observed that the same enzyme [i.e. an enzyme with the same EC (Enzyme Commission) classific...
Source: Enzyme and Microbial Technology - December 20, 2018 Category: Biotechnology Source Type: research

Laccase activity measurement by FTIR spectral fingerprinting
Publication date: Available online 18 December 2018Source: Enzyme and Microbial TechnologyAuthor(s): Valentina Perna, Andreas Baum, Heidi A. Ernst, Jane W. Agger, Anne S. MeyerAbstractLaccases (EC 1.10.3.2) are enzymes known for their ability to catalyze the oxidation of phenolic compounds using molecular oxygen as the final electron acceptor. Laccase activity is commonly determined by monitoring spectrophotometric changes (absorbance) of the product or substrate during the enzymatic reaction. Fourier Transform Infrared Spectroscopy (FTIR) is a fast and versatile technique where spectral evolution profiling, i.e. assessmen...
Source: Enzyme and Microbial Technology - December 18, 2018 Category: Biotechnology Source Type: research

Editorial Board
Publication date: February 2019Source: Enzyme and Microbial Technology, Volume 121Author(s): (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - December 14, 2018 Category: Biotechnology Source Type: research

Identification and characterization of a sterically robust phenylalanine ammonia-lyase among 481 natural isoforms through association of in silico and in vitro studies
In conclusion, finding models with the most reasonable stereo-chemical quality and lowest numbers of steric clashes would result in easier folding. Hence, in silico analyses of bulk data from natural origin will lead one to find an optimal model for in vitro studies and drug design.Graphical abstract (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - December 13, 2018 Category: Biotechnology Source Type: research

Horseradish Peroxidase Immobilized on the Magnetic Composite Microspheres for High Catalytic Ability and Operational Stability
Publication date: Available online 13 December 2018Source: Enzyme and Microbial TechnologyAuthor(s): Xueqiao Xie, Peng Luo, Juan Han, Tong Chen, Yun Wang, Yunfeng Cai, Qian LiuAbstractIn the present study, a novel and efficient immobilization for horseradish peroxidase (HRP) had been developed by using 6-arm magnetic composite microsphere (Fe3O4@PAA-6-arm-PEG-NH2) containing 6-arm polyethylene glycol (6-arm-PEG-NH2) and Fe3O4. The morphology and chemical properties of Fe3O4@PAA-6-arm-PEG-NH2 were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectra (FTIR), X-ray powder diffraction (XR...
Source: Enzyme and Microbial Technology - December 13, 2018 Category: Biotechnology Source Type: research

Functional expression of porcine interferon-α using a combinational strategy in Pichia pastoris GS115
Publication date: Available online 10 December 2018Source: Enzyme and Microbial TechnologyAuthor(s): Huahua He, Chao Zhai, Meng Mei, Yi Rao, Yao Liu, Fei Wang, Lixin Ma, Zhengbing Jiang, Guimin Zhang, Li YiAbstractPorcine interferon-α (pIFN-α) could be used as the vaccine adjuvant to enhance the antiviral ability of porcine in swine industry. In here, a combinational strategy integrating codon optimization, multiple gene insertion, strong AOX1 promoter, and efficient secretion signal sequence was developed to obtain high-level secreted pIFN-α in Pichia pastoris GS115. The codon optimized pIFN-α shar...
Source: Enzyme and Microbial Technology - December 11, 2018 Category: Biotechnology Source Type: research

An efficient thermostabilization strategy based on self-assembling amphipathic peptides for fusion tags
Publication date: February 2019Source: Enzyme and Microbial Technology, Volume 121Author(s): Weixin Zhao, Guocheng Du, Song LiuAbstractSelf-assembling amphipathic peptides (SAPs) have been used as stabilization tags to improve enzyme stability but do not function uniformly well with all target enzymes. Here, the key factors involved in SAPs stabilization were identified as the SAP length and linker length and flexibility, using S1 (AEAEAKAK)2 as an originated SAP and polygalacturonate lyase (PGL) as model protein. Biochemical analysis demonstrated that SAPs could induce loose protein oligomerization via intermolecular hydr...
Source: Enzyme and Microbial Technology - December 11, 2018 Category: Biotechnology Source Type: research

Optimization and modelling of Enzymatic Polymerization of ε-Caprolactone to Polycaprolactone Using Candida Antartica Lipase B with Response Surface Methodology and Artificial Neural Network
Publication date: Available online 5 December 2018Source: Enzyme and Microbial TechnologyAuthor(s): Harshini Pakalapatti, Mohammad Asad Tariq, Senthil Kumar ArumugasamyAbstractRecently enzymatic catalysts have replaced organic and organometallic catalysts in the synthesis of bio-resorbable polymers. Enzymatic polymerization is considered as an alternative to conventional polymerization as they are less toxic, environmental friendly and can operate under mild conditions. In this research, the enzymatic ring-opening polymerization (e-ROP) of e-caprolactone (e-CL) using Candida Antartica Lipase B (CALB) as catalyst to produce...
Source: Enzyme and Microbial Technology - December 6, 2018 Category: Biotechnology Source Type: research

Efficient immobilization of firefly luciferase in a metal organic framework: Fe-MIL-88(NH2) as a mighty support for this purpose
In conclusion, using Fe-MIL-88(NH2) as a support to immobilize luciferase established simple immobilization process of luciferase with and without linker and it improved the remaining activity significantly.Graphical abstract (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - December 6, 2018 Category: Biotechnology Source Type: research

Fructosylation of phenolic compounds by levansucrase from Gluconacetobacter diazotrophicus
Publication date: Available online 4 December 2018Source: Enzyme and Microbial TechnologyAuthor(s): Gema Núñez-López, Azucena Herrera-González, Lázaro Hernández, Lorena Amaya-Delgado, Georgina Sandoval, Anne Gschaedler, Javier Arrizon, Magali Remaud-Simeon, Sandrine MorelAbstractFructosylation can significantly improve the solubility, stability and bioactivity of phenolic compounds, increasing their health benefits. Levansucrase from Gluconacetobacter diazotrophicus (LsdA, EC 2.4.1.10) was found to transfer the fructosyl unit of sucrose to different classes of phenolic compounds. A...
Source: Enzyme and Microbial Technology - December 5, 2018 Category: Biotechnology Source Type: research

The effects of NaCl on enzyme encapsulation by zeolitic imidazolate frameworks-8
Publication date: Available online 3 December 2018Source: Enzyme and Microbial TechnologyAuthor(s): Shujin Pu, Xuan Zhang, Chengli Yang, Sidra Naseer, Xutong Zhang, Jie Ouyang, Dali Li, Junfang YangAbstractMetal-organic framework (MOF) has generated a lot of research interests for it can be employed as immobilization material for enzyme. There are many small molecules in enzyme solution during the extraction process, such as NaCl. It is important to study the effects of the small molecules on MOFs. Here we report a facile and efficient method to encapsulate (R)-1-phenylethanol dehydrogenase ((R)-PEDH) into zeolitic imidazo...
Source: Enzyme and Microbial Technology - December 4, 2018 Category: Biotechnology Source Type: research

Convenient preparation of sagittatoside B, a rare bioactive secondary flavonol glycoside, by recyclable and integrated biphase enzymatic hydrolysis
The objective of this study was to establish a novel catalysis system for convenient preparation of this compound based on recyclable and integrated biphase enzymatic hydrolysis. β-glucanase was selected from five commercial enzymes based on the best catalysis performance. After optimization of the conditions, the biphase system was constructed with propyl acetate and HAc-NaAc buffer (pH 4.5) (1:1, v/v) containing β-glucanase/epimedin B (1:2, w/w), and the hydrolysis was performed at 60 °C for 1 hr. Consequently, epimedin B was completely hydrolyzed to sagittatoside B, and 95.7% of the product w...
Source: Enzyme and Microbial Technology - December 4, 2018 Category: Biotechnology Source Type: research

A General Thermostabilization Strategy Based on Self-assembling Amphipathic Peptides for Fusion Tags
Publication date: Available online 17 November 2018Source: Enzyme and Microbial TechnologyAuthor(s): Weixin Zhao, Liming Liu, Guocheng Du, Song LiuAbstractSelf-assembling amphipathic peptides (SAPs) have been used as stabilization tags to improve enzyme stability but do not function uniformly well with all target enzymes. Here, the key factors that involved in SAPs stabilization were identified as the SAP length and linker length and flexibility, using S1 (AEAEAKAK)2 as an originated SAP and polygalacturonate lyase (PGL) as model protein. Biochemical analysis demonstrated that SAPs could induce the loose protein oligomeriz...
Source: Enzyme and Microbial Technology - November 18, 2018 Category: Biotechnology Source Type: research

Protein engineering to enhance keratinolytic protease activity and excretion in Escherichia coli and its scale-up fermentation for high extracellular yield
Publication date: Available online 10 November 2018Source: Enzyme and Microbial TechnologyAuthor(s): Zhen Fang, Chong Sha, Zheng Peng, Juan Zhang, Guocheng DuAbstractEscherichia coli is one kind of the simple and excellent biosystem to overexpress heterologous enzymes, such as keratinolytic protease, an excellent enzyme to hydrolyze keratin substrate for broad industrial application. However, protein expression in E. coli frequently faces some problems such as inactive and inclusion body formation. This work described a series of protein engineering strategies of N-terminal propeptide replacement and site-directed mutagene...
Source: Enzyme and Microbial Technology - November 11, 2018 Category: Biotechnology Source Type: research

Modeling of an electrochemical nanobiosensor in COMSOL Multiphysics to determine phenol in the presence of horseradish peroxidase enzyme
Publication date: Available online 3 November 2018Source: Enzyme and Microbial TechnologyAuthor(s): Amir Kaffash, Khosrow Rostami, Hamid R. ZareAbstractHorseradish peroxidase enzyme selectively oxidizes phenol to o-quinone that can be reduced electrochemically to catechol and generating a current response which is directly proportional to phenol concentration. In order to investigate the o-quinone enzymatic production and its electrochemical behavior, a 2-D model was developed for a nanochip biosensor in COMSOL Multiphysics. The oxidation rate of phenol to o-quinone was predicted by the developed model based on Michaelis-M...
Source: Enzyme and Microbial Technology - November 4, 2018 Category: Biotechnology Source Type: research

Editorial Board
Publication date: January 2019Source: Enzyme and Microbial Technology, Volume 120Author(s): (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - November 4, 2018 Category: Biotechnology Source Type: research

Deciphering the inhibition effect of thymoquinone on xanthine oxidase activity using differential pulse voltammetry in combination with theoretical studies
In this study, differential pulse voltammetry was used to investigate the inhibitory effect of thymoquinone (TQ) on the XO activity while the major problem was the overlap of the obtained signals. Thus, Parallel Factor Analysis (PARAFAC) was applied to extract the useful information. Also, docking was used to investigate how TQ and the active site of XO fit together. PARAFAC results based on the voltammetry studies revealed that TQ blocks the catalytic centers of XO, which leads to a decrease in the electrochemical signal of Mo center in XO. The results also indicated the dose-dependent inhibition of XO with TQ. Molecular ...
Source: Enzyme and Microbial Technology - November 3, 2018 Category: Biotechnology Source Type: research

Secretory overexpression of the endoglucanase by Saccharomyces cerevisiae via CRISPR-δ-integration and multiple promoter shuffling
In this study, to establish an efficient recombinant protein production technique in S. cerevisiae, the secretory production of recombinant protein endoglucanase II (TrEG) was tested. We developed 2 novel methods for TrEG production via clustered regularly interspaced short palindromic repeat (CRISPR) -δ-integration as well as multiple promoter shuffling, which involved the pre-breakdown of the δ-sequence by the CRISPR system and subsequent δ-integration as well as the conjugation of TrEG with various promoters and subsequent δ-integration, respectively. Moreover, simultaneous use of the CRISPR-&del...
Source: Enzyme and Microbial Technology - October 31, 2018 Category: Biotechnology Source Type: research

Simultaneous synthesis of L-DOPA and oxidation of D-amino acid by specific coupling of a peroxidase to D-amino acid oxidase
Publication date: Available online 29 October 2018Source: Enzyme and Microbial TechnologyAuthor(s): Yao Chen, Hao Chu, Wei Liu, Wei FengAbstract3,4 Dihydroxy phenyl l-alanine (L-DOPA) is the gold standard Parkinson's disease therapy. A heme-dependent peroxidase (HDP) catalyzes the ortho-hydroxylation of L-tyrosine to L-DOPA using H2O2 as the co-substrate. D-amino acid oxidase (DAAO) catalyzes the oxidative deamination of D-amino acids (e.g. D-alanine), and H2O2 is evolved. However, both the enzymes DAAO and HDP can be inactivated by H2O2 during the catalysis. In situ generation and utilization of H2O2 can siginificatly red...
Source: Enzyme and Microbial Technology - October 31, 2018 Category: Biotechnology Source Type: research

Cell entry of BmCPV can be promoted by tyrosine-protein kinase Src64B-like protein
Publication date: Available online 29 October 2018Source: Enzyme and Microbial TechnologyAuthor(s): Yiling Zhang, Liyuan Zhu, Guangli Cao, Mian Sahib Zar, Xiaolong Hu, Yuhong Wei, Renyu Xue, Chengliang GongAbstractBombyx mori cytoplasmic polyhedrosis virus (BmCPV) is a non-enveloped dsRNA virus, which specifically infect the midgut epithelium of B. mori. BmCPV enters permissive cells via clathrin-dependent endocytosis employing β1 integrin mediated internalization. Until now, the cell entry mechanism of BmCPV has not been known clearly. Here, we investigated whether tyrosine-protein kinase Src64B-like is involved in t...
Source: Enzyme and Microbial Technology - October 31, 2018 Category: Biotechnology Source Type: research

Efficient immobilization of firefly luciferase in Metal organic frameworks (MOFs): Fe-MIL-88(NH2) is a mighty support for this purpose
In conclusion, using Fe-MIL-88(NH2) as a support to immobilize luciferase established simple immobilization process of luciferase with and without linker and it improved the remaining activity significantly.Graphical abstract (Source: Enzyme and Microbial Technology)
Source: Enzyme and Microbial Technology - October 31, 2018 Category: Biotechnology Source Type: research

Evaluation of Angiotensin Converting Enzyme inhibitors by SPR biosensor and Theoretical Studies
Publication date: Available online 23 October 2018Source: Enzyme and Microbial TechnologyAuthor(s): Hafezeh Salehabadi, Khosro Khajeh, Bahareh Dabirmanesh, Mahmood Biglar, Massoud AmanlouAbstractSurface plasmon resonance (SPR) biosensor has been utilized for monitoring analyte-ligand interactions in modern drug discovery processes. SPR biosensors measure the change in refractive indexes over the course of analyte molecules' binding to a specific immobilized ligand on sensor chip. This effort highlights a comprehensive SPR study besides enzymatic assay for discovery of new Angiotensin Converting Enzyme (ACE) inhibitors via ...
Source: Enzyme and Microbial Technology - October 24, 2018 Category: Biotechnology Source Type: research

Effect of glucose depletion during the synthesis of galactooligosaccharides using a trienzymatic system
In this study the effect of glucose depletion using glucose oxidase and catalase, simultaneously to the synthesis of prebiotic galactooligosaccharides (GOS) by β-galactosidase was studied. Considering total GOS yield, a strong dependency on the source of β-galactosidase was found. Using an Aspergillus oryzae lactase, a small increase in GOS yield (from 50.0 ± 1.3 g/L to 54.1 ± 1.9 g/L) was noted. Due to the decreased rate of GOS disaccharide formation by the Kluyveromyces lactis enzyme, the total GOS yield was diminished (from 47.4 ± 0.1...
Source: Enzyme and Microbial Technology - October 23, 2018 Category: Biotechnology Source Type: research

The role of enzyme adsorption in the enzymatic degradation of an aliphatic polyester
Publication date: Available online 21 October 2018Source: Enzyme and Microbial TechnologyAuthor(s): Péter Polyák, Eszter Urbán, Gergely N. Nagy, Beáta G. Vértessy, Béla PukánszkyAbstractThe enzyme catalyzed degradation of poly(3-hydroxybutyrate) (PHB) is a two-step process consisting of the adsorption of the enzyme on the surface of a PHB substrate and the cleavage of ester bonds. A deactivated enzyme was prepared by point mutagenesis to separate the two steps from each other. Measurements carried out with active and inactive enzymes on PHB particles proved that mutagenesis ...
Source: Enzyme and Microbial Technology - October 22, 2018 Category: Biotechnology Source Type: research

Bioprocess optimization for the overproduction of catalytic domain of Ubiquitin-like protease 1 (Ulp1) from S. cerevisiae in E. coli Fed-batch culture
Publication date: Available online 21 October 2018Source: Enzyme and Microbial TechnologyAuthor(s): Babbal, Adivitiya, Shilpa Mohanty, Yogender Pal KhasaAbstractThe exploitation of SUMO (small ubiquitin-related modifier) fusion technology at a large scale for the production of therapeutic proteins with an authentic N-terminus is majorly limited due to the higher cost of ScUlp1 protease. Therefore, the cost-effective production of Saccharomyces cerevisiae Ulp1 protease catalytic domain (402-621 aa) was targeted via its cloning under strong T7 promoter with and without histidine tag. The optimization of cultivation condition...
Source: Enzyme and Microbial Technology - October 21, 2018 Category: Biotechnology Source Type: research