Fermented Citrus reticulata (ponkan) fruit squeezed draff that contains a large amount of 4 ′-demethylnobiletin prevents MK801-induced memory impairment

In this study using PC12D cells and cultured central nervous system neurons, we therefore examined whetherAspergillus kawachii-fermented citrus fruit squeezed draff could affect cAMP response element (CRE)- and choline acetyltransferase gene (ChAT) promoter region-mediated transcriptional activities relevant to memory formation and cholinergic function. Our current fermentation yielded approximately 80% nobiletin bioconversion, and a sample of hot-water extract of the fermented fruit squeezed draff was stronger than that of the unfermented one in facilitating CRE-mediated transcription in cultured hippocampal neurons as well as in PC12D cells. A sample of 0 –80% ethanol-eluted fraction of Diaion HP-20 column-adsorbed components of the preparation obtained by the fermentation concentration-dependently and more strongly facilitated CRE-mediated transcription than did the fraction of the unfermented one in both cell culture systems. In a separate study, this polymethoxyflavone-rich fraction of the fermented fruit squeezed draff showed a potent ability to facilitate CRE-mediated andChAT transcription in a co-culture of hippocampal neurons and basal forebrain neurons. Repeated oral gavage of mice with the fermented fraction sample prevented MK801-impaired memory formation in mice. These findings suggest that the 4 ′-demethylnobiletin-rich fraction prepared from theAspergillus-fermented ponkan squeezed draff has a potential anti-dementia effect.

Source: Journal of Natural Medicines - May 9, 2017 Category: Drugs & Pharmacology Source Type: research