Enhancement of adherence of Helicobacter pylori to host cells by virus: possible mechanism of development of symptoms of gastric disease

In this study, we analyzed whethersimian virus 5 (SV5) enhanced adherence ofH. pylori to adenocarcinoma epithelial cells (AGS).H. pylori in AGS (harboring SV5) and SV5-infected Vero cells, and an agglutination ofH. pylori mixed with SV5 were observed by light microscopy, scanning and transmission electron microscopies. The adherent rate ofH. pylori to SV5-infected Vero cells and treated with an anti-SV5 antibody was determined.H. pylori adhered to the surface of AGS cells near SV5 particles, as shown by scanning and transmission electron microscopies. The adherence ofH. pylori to SV5-infected Vero cells was significantly enhanced compared with that to Vero cells. In contrast, the adherence ofH. pylori to Vero cells was decreased by treatment with the anti-SV5 antibody. Agglutination ofH. pylori mixed with SV5 was observed by scanning and transmission electron microscopies. Agglutination did not occur when SV5 was treated with the anti-SV5 antibody before mixing. These findings demonstrated that SV5 enhanced the adherence ofH. pylori to host cells, suggesting that a persistently infected virus may be a factor enhancing the pathogenicity ofH. pylori in humans.
Source: Medical Molecular Morphology - Category: Molecular Biology Source Type: research