Leptin and Remyelination

Leptin Promotes Proliferation of OPCs Demyelination occurs in many diseases of the Central Nervous System (CNS) including Multiple Sclerosis and Parkinson ' s Disease. Here researchers show that Leptin plays a role in the proliferation of Oligodendrocyte Precursor Cells (OPCs): These cells are critical for keeping the myelin sheath on Neurons of the CNS healthy and happy. Ken Matoba, Rieko Muramatsu& Toshihide Yamashita. Leptin sustains spontaneous remyelination in the adult central nervous system. Scientific Reports 7, Article number: 40397 (2017) doi:10.1038/srep40397.OurLepRB Antibody is used in this study to stain OPCs.Figures: (a) Representative image of cultured OPCs stained with antibodies against LepRb (green) and PDGFR α (red). Scale bar: 25 μm. (b) Relative BrdU incorporation into the OPC obtained from the brain (left graph) and spinal cord (right graph). Cells were treated with recombinant leptin for 48 h (n = 4). (Left graph) P = 0.005993 (control vs 10 ng/mL), 0.045616 (control vs 100 ng/mL), (Right graph) P = 0.004456 (control vs 10 ng/mL), 0.017859 (control vs 100 ng/mL). (c) Relative BrdU incorporation into the OPC after leptin stimulation (10 ng/ml) with U0126 (20 μM), a MEK inhibitor (n = 4 for brain OPCs, n = 3 for spinal cord OPCs). (Left graph) P = 0.019753 (control vs leptin), 0.039433 (lepti n vs leptin + U0126), (Right graph) P = 0.045545 (control vs leptin), 0.04486 (leptin vs leptin + U0126). (d) Representative images of western blotting ...
Source: Neuromics - Category: Neuroscience Tags: LepRb antibody Leptin Multiple Sclerosis Oligodendrocyte Precursor Cells OPCs Parkinson ' s Disease Source Type: news